Supplemental Table I. Genes concomitantly modulated by activated Notch1 in mouse and human primary keratinocytes. Genes up-regulated by Notch1 both in human and in mouse keratinocytes M access. # Hu access. # NIC/GFP M NIC/GFP Hu Description X60367 M11433 20.72 4.23 Retinol binding protein 1 AW049647 AF070523 2.28 8.10 X99921 AI541308 3.46 6.32 S100 calcium-binding protein A13 M28383 U05596 6.02 3.38 Solute carrier family 4 (anion exchanger), member 3 U14135 M14648 2.96 5.91 Integrin alpha V (Cd51) X99921 AI541308 2.06 6.32 S100 calcium-binding protein A13 D88533 D82326 3.58 4.48 NBAT M60057 J03802 3.24 4.55 Parathyroid hormone- like peptide AJ237939 U43185 2.84 4.13 partial STAT5B gene, exons 6-9 U61085 X91220 2.11 4.62 thiazide-sensitive Na-Cl cotransporter mRNA AA882416 D87127 2.54 3.88 L22545 AF018081 2.48 3.79 Procollagen, type XVIII, alpha 1 AW121732 L78833 2.78 3.23 AW121732 L78833 2.78 3.16 U90435 AI807620 2.20 3.68 flotillin AB022022 AL031177 2.07 3.61 gankyrin X14943 Z21488 3.57 2.07 Contactin 1 AJ223855 AJ010063 2.14 3.20 telethonin AW060546 X70944 2.10 3.22 U14135 M14648 2.96 2.20 Integrin alpha V (Cd51) U12961 M81600 2.92 2.20 NAD(P)H menadione oxidoreductase 1, dioxin inducible X98207 U50079 2.15 2.91 histone deacetylase X07888 M11058 2.90 2.15 HMGCR gene for 3- hydroxy-3-methylglutaryl coenzyme A reductase L18888 L10284 2.45 2.54 Calnexin X98207 D50405 2.15 2.79 histone deacetylase AW046032 M62424 2.30 2.62 X13460 Y00097 2.67 2.24 Annexin VI, p68 AC002397 U72511 2.16 2.61 U12961 M81600 2.56 2.20 NAD(P)H menadione oxidoreductase 1, dioxin inducible Z68618 M95787 2.26 2.30 Transgelin AA615429 AB020631 2.17 2.38 AF037312 L78207 2.03 2.37 AC002397 U47924 2.16 2.21 U43541 X79683 2.04 2.26 Laminin, beta 2 Genes down-regulated by Notch1 both in human and in mouse keratinocytes M access. # Hu access. # NIC/GFP M NIC/GFP Hu Description L00039 V00568 0.20 0.06 Myelocytomatosis oncogene Y12881 Z14000 0.10 0.18 transcription repressor Ring1A AB010152 Y16961 0.19 0.10 p73H AF064088 S81439 0.30 0.00 transcription factor GIF U17291 M36711 0.25 0.06 transcription factor AP-2 (AP-2) gene AF061260 AB017563 0.28 0.06 immunosuperfamily protein Bl2 AF064088 AF050110 0.30 0.04 transcription factor GIF AF064088 S81439 0.40 0.00 transcription factor GIF J04103 AF017257 0.38 0.03 E26 avian leukemia oncogene 2 Y12881 AL031228 0.10 0.31 transcription repressor Ring1A AI852829 AL022238 0.37 0.04 M20658 M27492 0.37 0.05 Interleukin 1 receptor AI846938 AF055001 0.34 0.08 AW123880 Z93930 0.38 0.04 AF064088 AF050110 0.40 0.04 transcription factor GIF AI132380 AB023187 0.35 0.09 AB006758 AB006755 0.21 0.24 Pcdh7 BH-protocadherin-a AA110657 U42390 0.33 0.12 AW046449 D82351 0.32 0.14 L00039 V00568 0.20 0.26 Myelocytomatosis oncogene M32490 Y11307 0.29 0.17 Insulin-like growth factor binding protein 10 AJ011033 AF054506 0.09 0.38 kcc2 gene M88242 U04636 0.01 0.46 Mouse glucocortoid-regulated inflammatory prostaglandin G U67187 L13463 0.22 0.25 G protein signaling regulator RGS2 (rgs2) U20344 U70663 0.33 0.16 Gut enriched Kruppel-like factor AF083464 AL096744 0.35 0.14 DNA polymerase zeta catalytic subunit AW125858 U14391 0.28 0.22 X96639 S79639 0.39 0.12 Exostoses (multiple) 1 AW120868 AL080156 0.48 0.03 AW046563 M65254 0.40 0.12 AF061260 AB017563 0.46 0.06 immunosuperfamily protein Bl2 AJ010045 AJ010046 0.47 0.05 Rho guanine nucleotide- exchange factor AW045443 U49020 0.38 0.15 U12763 AL022310 0.24 0.30 Tax-transcriptionally activated glycoprotein 1 ligand X54149 N95168 0.11 0.43 Myeloid differentiation primary response gene 118 X01026 J02843 0.13 0.41 Mouse fragment for repetitive element B2 AW123880 Z93930 0.38 0.17 AF100956 Z14000 0.37 0.18 major histocompatibility locus class II region; Fas-binding protein Daxx (DAXX) gene AF100956 AA808961 0.37 0.19 major histocompatibility locus class II region; Fas-binding protein Daxx (DAXX) gene L26479 J04617 0.29 0.30 Eukaryotic translation elongation factor 1 alpha 2 M26071 J02931 0.35 0.25 Coagulation factor III X75427 X17033 0.41 0.20 Integrin alpha 2 (Cd49b) X65138 L36645 0.43 0.18 Eph receptor A4 X60676 D83174 0.46 0.16 HSP47 M73696 L20859 0.45 0.17 Murine Glvr-1 U37501 AB011105 0.39 0.23 Laminin U77615 W29036 0.24 0.40 Cone-rod homeobox containing gene AF005886 D50310 0.31 0.34 cyclin I AF020200 X59841 0.38 0.26 transcription factor PBX3b (PBX3b) AA611766 D14520 0.28 0.37 AF031035 AF098462 0.20 0.45 stanniocalcin (Stc2) Y08701 Y09703 0.33 0.32 Pinin protein AF100956 D42040 0.37 0.28 major histocompatibility locus class II region; Fas-binding protein Daxx (DAXX) gene U13174 U30246 0.21 0.44 Solute carrier family 12 AF020200 X59841 0.39 0.26 transcription factor PBX3b (PBX3b) L26479 W28170 0.29 0.37 Eukaryotic translation elongation factor 1 alpha 2 AB006036 U88666 0.45 0.22 Serine X60664 M26061 0.31 0.36 CGMP phosphodiesterase alpha AF100956 AF006041 0.37 0.31 major histocompatibility locus class II region; Fas-binding protein Daxx (DAXX) gene AI845578 AJ224326 0.42 0.26 AI846890 Z75331 0.40 0.29 AI843466 AI557272 0.39 0.30 D83966 U12128 0.34 0.36 Protein tyrosine phosphatase AF109905 AJ012008 0.35 0.36 major histocompatibility locus class III regions Hsc70t gene AB025966 AB002310 0.39 0.33 KIAA312p D21831 AL031228 0.41 0.31 Retinoid X receptor beta AI843738 U50383 0.39 0.34 AI852829 AL022238 0.37 0.35 AW123479 D30612 0.33 0.40 AW124113 AA135683 0.32 0.42 AI842675 M80254 0.34 0.40 AB021961 X02469 0.34 0.41 mutant p53 AF017085 AF015553 0.42 0.34 BAP-135 homolog (Diws1t) AW121185 AB002323 0.42 0.33 D90374 M80261 0.50 0.27 Mouse APEX nuclease AB021961 X02469 0.36 0.41 mutant p53 AF004108 U40391 0.29 0.48 arylalkylamine N- acetyltransferase AF005886 D50310 0.44 0.34 cyclin I M60474 D10522 0.40 0.38 Myristoylated alanine rich protein kinase C substrate AF007552 AF007551 0.28 0.49 Bet1p homolog (mbet1) AI839918 U09510 0.39 0.40 AI839918 U09510 0.39 0.40 L10385 L10386 0.34 0.46 transglutaminase E (TGASE3) AF017085 U77948 0.42 0.38 BAP-135 homolog (Diws1t) AI182203 AF054506 0.42 0.38 AI197161 U88629 0.39 0.42 AI845935 AL031282 0.37 0.44 AF109905 AJ012008 0.47 0.36 major histocompatibility locus class III regions Hsc70t gene AW125800 AB028986 0.44 0.39 AW047978 AF020760 0.47 0.37 U32107 L02870 0.49 0.36 Procollagen AF085192 AF070616 0.43 0.42 neural visinin-like protein 3 (NVP- 3) AI117848 U15128 0.47 0.39 AF100956 U41068 0.37 0.50 major histocompatibility locus class II region; Fas-binding protein Daxx (DAXX) gene AW122255 AB007931 0.49 0.40 AF056187 X04434 0.48 0.40 insulin-like growth factor I receptor U29055 AL031282 0.46 0.44 Guanine nucleotide binding protein AA867340 D38521 0.48 0.43 AI854811 X55504 0.44 0.47 AI849620 M63180 0.48 0.44 AW050034 D28476 0.42 0.50 AB006036 U88666 0.45 0.47 Serine M31690 X01630 0.46 0.49 Arginosuccinate synthetase 1 AB005132 D45906 0.49 0.46 LIM motif-containing protein kinase 2 U40796 L20046 0.49 0.46 Xeroderma pigmentosum J04627 X16396 0.47 0.50 Mouse NAD-dependent methylenetetrahydrofolate dehydrogenase- methenyltetrahydrofolate cyclohydrolase RNA was prepared from primary mouse and human keratinocytes infected with the Ad- NIC versus Ad-GFP adenoviruses and used for cRNA probe preparation and hybridization to Affymetrix U74A and U95A gene chips, respectively. Only positive flurorescence intensity values were considered. Human and mouse gene average intensities were scaled as described (Tusher et al. 2001) and genes with scaled intensity difference > 500 and changes in gene expression of >2 folds (in NIC vs GFP comparisons) in both duplicates of each hybridization reaction were considered as significant. Shown are the accession numbers for both mouse and human genes as well as gene description whenever known. The relative expression levels of genes in Ad-NIC versus Ad-GFP – infected cells of both mouse (NIC/GFP M) and human (NIC/GFP Hu) origin is indicated. Supplemental Table II. Selective Notch control of interferon responsive genes. Accession Function Gene GFP N1 N1/GFP Interferons and related cytokines NM_002416 chemokine (C-X-C motif) ligand 9 CXCL9 40.2 68.3 1.7 NM_001565 chemokine (C-X-C motif) ligand 10 CXCL10 47.5 52.3 1.1 NM_000600 interleukin 6 (interferon, beta 2) IL6 66.8 80.2 1.2 NM_001562 interleukin 18 (interferon-gamma-inducing factor) IL18 959.3 383.7 0.4 NM_002172 interferon, alpha 14 IFNA14 48.3 62.8 1.3 NM_021057 interferon, alpha 7 IFNA7 27.5 24.8 0.9 NM_002171 interferon, alpha 10 IFNA10 24.7 19.8 0.8 NM_006900 interferon, alpha 13 IFNA13 33.0 29.7 0.9 AF030514 chemokine (C-X-C motif) ligand 11 CXCL11 29.4 8.8 0.3 M29383 interferon, gamma IFNG 23.2 20.9 0.9 M38289 interferon, alpha 17 IFNA17 111.1 133.3 1.2 NM_002169 interferon, alpha 5 IFNA5 8.4 33.6 4.0 NM_021068 interferon, alpha 4 IFNA4 39.9 35.9 0.9 NM_004847 allograft inflammatory factor 1 AIF1 32.6 71.7 2.2 Interferon receptors NM_005534 interferon gamma receptor 2 (interferon gamma transducer 1) IFNGR2 1567.2 1880.6 1.2 NM_000416 interferon gamma receptor 1 IFNGR1 756.1 982.9 1.3 NM_000629 interferon (alpha, beta and omega) receptor 1 IFNAR1 50.3 40.2 0.8 NM_000874 interferon (alpha, beta and omega) receptor 2 IFNAR2 99.7 199.4 2.0 L41944 interferon (alpha, beta and omega) receptor 2 IFNAR2 29.9 44.9 1.5 AF056979 interferon gamma receptor 1 IFNGR1 280.7 561.4 2.0 Interferon regulators NM_002534 2',5'-oligoadenylate synthetase 1, 40/46kDa OAS1 314.8 157.4 0.5 NM_016817 2'-5'-oligoadenylate synthetase 2, 69/71kDa OAS2 765.1 382.6 0.5 NM_006187 2'-5'-oligoadenylate synthetase 3, 100kDa OAS3 514.1 308.5 0.6 AF063612 2'-5'-oligoadenylate synthetase-like OASL 142.4 128.2 0.9 BC002704 signal