Thermo Scientific Chromatography Columns and Consumables Thermo Scientific Chromatography Columns and Consumables 2014-2015 2014-2015 Your world of chromatography predictability LC Columns and Accessories As a leader in LC column technology including silica, polymer and porous graphitic carbon manufacturing, bonded phase production and column packing for over 35 years, you can rely on the quality of Thermo Scientific HPLC products. Here we 2014-2015 showcase our latest and most comprehensive range of innovative columns, accessories and equipment for fast LC, analytical HPLC and biomolecule separations. Chromatography Columns and Consumables Thermo Scienti c Thermo Scienti 4 LC Columns and Accessories Section Contents Introduction . 4-001 LC Accessories. 4-158 Column Selection. 4-002 LC Equipment . 4-186 Column Protection . 4-016 LC Reagents . 4-193 Columns for Fast and Analytical LC . 4-022 LC and LC-MS Instrument Parts . 4-209 Columns for Biomolecules . 4-110 Technical Information. 4-231 Featured Products Accucore Columns Syncronis Columns Hypersil GOLD Columns Ultimate core performance - Consistent, reproducible separations, Outstanding peak shape for speed and selectivity combined column after column, time after time your separations PAGE 4-042 PAGE 4-074 PAGE 4-024 MAbPac Columns Acclaim PepMap Columns Viper Connectors Unrivalled resolution and ef ciency The standard for peptide Simple, dead volume free plumbing in the analysis of protein variants separations in proteomics of HPLC and UHPLC systems PAGE 4-126 PAGE 4-149 PAGE 4-158 www.thermoscienti c.com/chromatography 4-001 LC Column Selection Information in the following section will help you make an informed decision on the appropriate HPLC column for your application, based on stationary phase use, analyte properties, LC-MS requirements or USP specifications. You will also find a useful table of Thermo Scientific phases with specifications, as well as recommended Thermo Scientific alternatives for other popular columns. Refer to the Advanced User Graphic (AUG) on the The Pore Size is shown, with larger pore size columns Hydrophobicity corresponding product page (illustrated to the right) being more applicable to larger analytes such as Low High for more help and information on general purpose proteins or peptides. The percentage Carbon Load pH Range column selection. is related to the hydrophobicity. Below the icon, you 012 to 8 4 The AUG will show you Hydrophobicity which gives will see the particle sizes available, as well as the Pore Size the relative retention on the column. Generally, the USP code. These graphics are designed to allow you to quickly compare the main characteristics of multiple 0 80Å 300 higher the hydrophobicity, the greater the retention Carbon Load (%) stationary phases, allowing you to choose quickly the LC Columns and Accessories of neutral compounds and the higher the organic 025 5 content in the mobile phase. A lower value indicates most appropriate stationary phase for your analysis. Particle Size 2.6µm USP L11 a need for higher aqueous mobile phases to achieve For additional help in column selection, please comparable retention and resolution. The see the back cover to contact our expert Technical Advanced User Graphic (AUG) recommended pH Range for the column is illustrated, Support and tap into our expertise to help make outside of which column lifetimes will diminish. the best choice for your application. Common HPLC Phases and Their Uses Common Alternative Functional Normal Reverse Ion HILIC Application Name Name Group Phase Phase Exchange Silica Silica -OH • • Non-polar and moderately polar organic compounds. C1 SAS -(CH ) Least retentive of all alkyl group bonded phases for non-polar solutes. 3 3 • Typically used for moderately polar and multi-functional compounds. C4 Butyl -C4H9 • Shorter retention than C8, C18. Separation of peptides and proteins. C8 MOS -C H Less retentive than C18; normally used for small peptides and 8 17 • proteins, pharmaceuticals, steroids, environmental samples. C18 ODS -C H Most retentive of the alkyl-bonded phases. Used widely for 18 37 • pharmaceuticals, steroids, fatty acids, phthalates, environmental etc. Cyano CPS, CN -(CH2)3CN Unique selectivity for polar compounds, more suitable than base silica for normal phase gradient separations. When used in reversed •• phase, the selectivity is different to that of the C8 and C18 phases. Useful for a wide range of pharmaceutical applications and for mixtures of very different solutes. Amino APS -(CH2)3NH2 HILIC: Carbohydrate analysis and other polar compounds. 2014-2015 •••• Weak anion exchange: anions and organic acids. Normal Phase: Alternative selectivity to silica. Good for aromatics. Phenyl -(CH3)C6H5 • Aromatic compounds and moderately polar compounds. Penta uo- PFP -C F Extra selectivity and retention for halogenated, polar compounds 6 5 • rophenyl and isomers. Diol -(CH ) Reversed Phase: Proteins, peptides. 2 2O •• • CH2(CH2OH)2 Normal Phase: Similar selectivity to silica, but less polar. SCX Strong Cation -RSO H- Organic bases. 3 • Exchanger SAX Strong Anion -RN+(CH ) Organic acids, nucleotides and nucleosides. 3 3 • Exchanger AX Anion Exchanger -(CH2CH2NH-)n Organic acids, nucleotides and oligonucleotides. Polyethyleneimine • Chromatography Columns and Consumables (PEI) Porous PGC 100% carbon Particularly useful for the separation of highly polar compounds that graphitic are dif cult to retain using conventional silica based columns; •• carbon separation of structurally similar compounds (e.g., isomers, diastereoisomers). Thermo Scienti c Thermo Scienti 4-002 HPLC Stationary Phase Column Selection LC Columns and Accessories Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis and how the analyte(s) will interact with the surface of the chromatographic phase. To aid column selection, the following guide may be useful. Non-polar Analyte Solubilty Analyte Chemical Properties Mode of Separation Recommended Phase Acclaim Mixed-Mode WAX-1 4-069 Acidic pK > 2 Anion exchange / Reversed-phase mixed mode a Acclaim Trinity P1 4-091 Accucore C18 4-045 Accucore RP-MS 4-044 Accucore C8 4-046 Accucore C30 4-052 Accucore Phenyl-Hexyl 4-049 Acclaim 120 C18 4-061 Acclaim 120 C8 4-062 Non-polar Reversed phase >> Acclaim C30 4-064 Column Selection Acclaim Phenyl-1 4-063 Hypersil GOLD 4-026 Hypersil GOLD C8 4-027 Hypersil GOLD C4 4-028 Neutral Syncronis C18 4-075 Syncronis C8 4-076 Accucore aQ 4-047 Accucore Polar Premium 4-048 Accucore PFP 4-051 Accucore Phenyl-X 4-050 Acclaim PolarAdvantage 4-065 Polar Moderately-polar Reversed phase solvents Acclaim PolarAdvantage II 4-066 Hypersil GOLD aQ 4-029 Hypersil GOLD PFP 4-030 Hypersil GOLD Phenyl 4-031 Syncronis aQ 4-077 Syncronis Phenyl 4-078 Accucore C18 4-045 Accucore RP-MS 4-044 Accucore C8 4-046 Accucore C30 4-052 Accucore Phenyl-Hexyl 4-049 Acclaim 120 C18 4-061 Acclaim 120 C8 4-062 Reversed phase Acclaim C30 4-064 Basic pK < 10 a Acclaim Phenyl-1 4-063 Hypersil GOLD 4-026 Hypersil GOLD C8 4-027 Hypersil GOLD C4 4-028 Syncronis C18 4-075 Syncronis C8 4-076 Acclaim Mixed-Mode WCX-1 4-070 Cation exchange / Reversed-phase mixed mode Acclaim Trinity P1 4-091 www.thermoscienti c.com/chromatography 4-003 HPLC Stationary Phase Column Selection continued Polar Analyte Solubilty Analyte Chemical Properties Mode of Separation Recommended Phase Polar retention effect on graphite Hypercarb 4-084 Hypersil GOLD AX 4-034 Anion exchange Hypersil GOLD SAX 4-035 Acidic pKa < 2 Accucore Urea-HILIC 4-054 Accucore 150-Amide-HILIC 4-146 HILIC Acclaim HILIC-10 4-067 Syncronis HILIC 4-081 Polar retention effect on graphite Hypercarb 4-084 Accucore HILIC 4-053 Acclaim Mixed-Mode HILIC-1 4-068 LC Columns and Accessories Acclaim Mixed-Mode WAX-1 4-069 Acclaim Mixed-Mode WCX-1 4-070 Polar Neutral solvents HILIC Acclaim Trinity P1 4-091 Hypersil GOLD Amino 4-033 Hypersil GOLD HILIC 4-037 Syncronis Amino 4-079 Syncronis HILIC 4-081 Polar retention effect on graphite Hypercarb 4-084 Acclaim Mixed-Mode WCX-1 4-070 Cation exchange Acclaim Trinity P1 4-091 Basic pKa > 10 Accucore Urea-HILIC 4-054 Accucore 150-Amide-HILIC 4-146 HILIC Acclaim HILIC-10 4-067 Syncronis HILIC 4-081 Hypersil GOLD Amino 4-033 Hypersil GOLD CN 4-032 Non-polar Normal phase Hypersil GOLD Silica 4-036 solvents Syncronis Amino 4-079 Syncronis Silica 4-080 The above table recommends columns for the separation of small molecule analytes. Column recommendations for the separation of biomolecules can be found in the table on page 4-111 2014-2015 Chromatography Columns and Consumables Thermo Scienti c Thermo Scienti 4-004 Column Format Selection for LC-MS LC Columns and Accessories The Thermo Scientific range offers a broad array of column designs and stationary phases optimized for LC-MS applications. Use the following table to help you choose your column format to best meet your application needs. A variety of HPLC column hardware configurations are available, designed to give superior results for high speed, high sensitivity, high efficiency and convenience. A wide range of particle and monolithic stationary phases allows choices for optimized selectivity. Column Hardware Selection for LC-MS LC-MS Application Column Hardware Design Description High throughput analysis Javelin HTS columns Direct-connection columns Slim design, 20mm length, 1mm to 4.6mm ID High sensitivity analysis Acclaim PepMap nano, capillary Nano, capillary and micro columns 0.075mm to 1mm ID; 50 to 250mm length and micro columns nanoViper format