Anti-CLCA1 (C-terminal half) monoclonal antibody, clone 5E3.3 (CABT-B1341) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Specificity Detects the 90 kDa processed/cleaved 4-transmembrane glycoprotein, but not the cleaved 37-41 kDa C-terminal fragment. Immunogen GST-tagged recombinant protein corresponding to an internal fragment from the C-terminal half of human CLCA1. Isotype IgG1, κ Source/Host Mouse Species Reactivity Human Clone 5E3.3 Purification Protein G Purified Conjugate Unconjugated Applications WB, IHC Epitope Internal (C-terminal half). Molecular Weight ~83 kDa observed. 125 kDa glycosylated precursor form, 90 kDa and 37-41 kDa processed/cleaved fragements reported. Format Liquid Concentration Please refer to lot specific datasheet. Size 100 μg Buffer 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Preservative 0.05% Sodium Azide Storage Stable for 1 year at 2-8°C from date of receipt. BACKGROUND 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Introduction Calcium-activated chloride channel regulator 1 (UniProt A8K7I4; also known as CaCC-1, Calcium-activated chloride channel family member 1, Calcium-activated chloride channel protein 1, hCaCC-1, hCLCA1) is encoded by the CLCA1 (also known as CACC, CACC1, CLCC1, GOB5) gene (Gene ID 1179) in human. CLCA1 belongs to the calcium-activated chloride channel regulator (previously known as chloride channel calcium activated or CLCA) family of self-cleaving, secreted metalloproteases that activate calcium-dependent chloride currents (ICaCC) in mammalian cells. Originally thought to be calcium-activated chloride channels (CaCCs), CLCA proteins were later identified as secreted CaCCs modulators. TMEM16A (a.k.a. Anoctamin1 and DOG1) is a CaCC expressed in airway epithelia and smooth muscle. The secreted CLCA1 is reported to physically interact with TMEM16A and modulate TMEM16A- dependent calcium-activated chloride currents. Adding CLCA1 to cells causes more TMEM16A channels to appear in the cell membrane, which increases the flow of chloride ions. GENE INFORMATION Entrez Gene ID 1179 UniProt ID A8K7I4 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.