Table 4. V. cholerae FLEXGene ORF collection Reference Clone protein PlasmID clone GenBank Locus tag Symbol accession identifier FLEX clone name accession Product name VC0001 NP_062585 VcCD00019918 FLH200476.01F DQ772770 hypothetical protein VC0002 mioC NP_062586 VcCD00019938 FLH200506.01F DQ772771 mioC protein VC0003 thdF NP_062587 VcCD00019958 FLH200531.01F DQ772772 thiophene and furan oxidation protein ThdF VC0004 yidC NP_062588 VcCD00019970 FLH200545.01F DQ772773 inner membrane protein, 60 kDa VC0005 NP_062589 VcCD00061243 FLH236482.01F DQ899316 conserved hypothetical protein VC0006 rnpA NP_062590 VcCD00025697 FLH214799.01F DQ772774 ribonuclease P protein component VC0007 rpmH NP_062591 VcCD00061229 FLH236450.01F DQ899317 ribosomal protein L34 VC0008 NP_062592 VcCD00019917 FLH200475.01F DQ772775 amino acid ABC transporter, ATP-binding protein VC0009 NP_062593 VcCD00019966 FLH200540.01F DQ772776 amino acid ABC transproter, permease protein VC0010 NP_062594 VcCD00019152 FLH199275.01F DQ772777 amino acid ABC transporter, periplasmic amino acid-binding portion VC0011 NP_062595 VcCD00019151 FLH199274.01F DQ772778 hypothetical protein VC0012 dnaA NP_062596 VcCD00017363 FLH174286.01F DQ772779 chromosomal DNA replication initiator DnaA VC0013 dnaN NP_062597 VcCD00017316 FLH174063.01F DQ772780 DNA polymerase III, beta chain VC0014 recF NP_062598 VcCD00019182 FLH199319.01F DQ772781 recF protein VC0015 gyrB NP_062599 VcCD00025458 FLH174642.01F DQ772782 DNA gyrase, subunit B VC0016 NP_229675 VcCD00019198 FLH199346.01F DQ772783 hypothetical protein VC0017 NP_229676 VcCD00019145 FLH199266.01F DQ772784 hypothetical protein VC0018 ibpA NP_229677 VcCD00061122 FLH234790.01F DQ899318 16 kDa heat shock protein A VC0019 avtA NP_229678 VcCD00025656 FLH214606.01F DQ772785 valine-pyruvate aminotransferase VC0020 glyS NP_229679 VcCD00017428 FLH174578.01F DQ772786 glycyl-tRNA synthetase, beta chain VC0021 glyQ NP_229680 VcCD00019175 FLH199311.01F DQ772787 glycyl-tRNA synthetase, alpha chain VC0022 NP_229681 VcCD00019146 FLH199267.01F DQ772788 conserved hypothetical protein VC0023 NP_229682 VcCD00019167 FLH199296.01F DQ772789 NADH dehydrogenase subunit II-related protein VC0024 NP_229683 VcCD00019165 FLH199294.01F DQ772790 conserved hypothetical protein VC0025 NP_229684 VcCD00019163 FLH199292.01F DQ772791 hypothetical protein VC0026 NP_229685 VcCD00019170 FLH199305.01F DQ772792 zinc-binding alcohol dehydrogenase VC0027 ilvA NP_229686 VcCD00025657 FLH214610.01F DQ772793 threonine dehydratase VC0028 ilvD NP_229687 VcCD00025658 FLH214615.01F DQ772794 dihydroxy-acid dehydratase VC0029 ilvE NP_229688 VcCD00019168 FLH199297.01F DQ772795 branched-chain amino acid amiotransferase VC0030 ilvM NP_229689 VcCD00061095 FLH234718.01F DQ899319 acetolactate synthase II, small subunit VC0031 ilvG NP_229690 VcCD00019197 FLH199345.01F DQ772796 acetolactate synthase II, large subunit VC0032 NP_229691 VcCD00019193 FLH199339.01F DQ772797 ComM-related protein VC0033 NP_229692 VcCD00025652 FLH214586.01F DQ772798 conserved hypothetical protein VC0034 tpcG NP_229693 VcCD00019144 FLH199265.01F DQ772799 thiol:disulfide interchange protein VC0035 NP_229694 VcCD00019181 FLH199317.01F DQ772800 conserved hypothetical protein VC0036 NP_229695 VcCD00025587 FLH199335.01F DQ772801 FixG-related protein VC0037 NP_229696 VcCD00025777 FLH218584.01F DQ772802 conserved hypothetical protein VC0038 NP_229697 VcCD00061076 FLH234670.01F DQ899320 hypothetical protein VC0039 NP_229698 VcCD00019160 FLH199287.01F DQ772803 SpoOM-related protein VC0040 NP_229699 VcCD00025571 FLH199174.01F DQ772804 hemolysin, putative VC0041 NP_229700 VcCD00025573 FLH199178.01F DQ772805 conserved hypothetical protein VC0042 trkH-1 NP_229701 VcCD00019131 FLH199245.01F DQ772806 potassium uptake protein TrkH VC0043 trkA NP_229702 VcCD00019128 FLH199238.01F DQ772807 potassium uptake protein TrkA VC0044 sun NP_229703 VcCD00019125 FLH199232.01F DQ772808 sun protein VC0045 fmt NP_229704 VcCD00017440 FLH174621.01F DQ772809 methionyl-tRNA formyltransferase VC0046 def-1 NP_229705 VcCD00017425 FLH174560.01F DQ772810 polypeptide deformylase VC0047 NP_229706 VcCD00060971 FLH199227.01F DQ899321 conserved hypothetical protein VC0048 smf NP_229707 VcCD00061101 FLH234736.01F DQ899322 smf protein VC0049 smg NP_229708 VcCD00025838 FLH218733.01F DQ772811 smg protein VC0050 NP_229709 VcCD00025841 FLH218741.01F DQ772812 DNA topoisomerase I-related protein VC0051 purK NP_229710 VcCD00025581 FLH199225.01F DQ772813 phosphoribosylaminoimidazole carboxylase, ATPase subunit VC0052 purE NP_229711 VcCD00061059 FLH234628.01F DQ899323 phosphoribosylaminoimidazole carboxylase, catalytic subunit VC0053 NP_229712 VcCD00061063 FLH234638.01F DQ899324 hypothetical protein VC0054 NP_229713 VcCD00019136 FLH199252.01F DQ772814 Sua5-YciO-YrdC family protein VC0055 hemF NP_229714 VcCD00025578 FLH199202.01F DQ772815 coproporphyrinogen III oxidase, aerobic VC0056 aroE NP_229715 VcCD00019118 FLH199194.01F DQ772816 shikimate 5-dehydrogenase VC0057 NP_229716 VcCD00025826 FLH218701.01F DQ772817 conserved hypothetical protein VC0058 NP_229717 VcCD00061065 FLH234644.01F DQ899325 carbonic anhydrase, family 3 VC0060 crcB NP_229719 VcCD00019124 FLH199220.01F DQ772818 crcB protein VC0061 thiC NP_229720 VcCD00019137 FLH199255.01F DQ772819 thiamin biosynthesis protein ThiC VC0062 thiE NP_229721 VcCD00019126 FLH199234.01F DQ772820 thiamin-phosphate pyrophosphorylase VC0063 thiF NP_229722 VcCD00025576 FLH199192.01F DQ772821 thiF protein VC0064 thiS NP_229723 VcCD00025822 FLH218691.01F DQ772822 thiS protein VC0065 thiG NP_229724 VcCD00061091 FLH234708.01F DQ899326 thiG protein VC0066 thiH NP_229725 VcCD00019123 FLH199219.01F DQ772823 thiH protein VC0067 NP_229726 VcCD00061124 FLH234795.01F DQ899327 aminopeptidase P VC0068 NP_229727 VcCD00019120 FLH199211.01F DQ772824 transcriptional regulator, LysR family VC0069 NP_229728 VcCD00025835 FLH218727.01F DQ772825 multidrug resistance protein, putative VC0070 NP_229729 VcCD00019064 FLH199107.01F DQ772826 hypothetical protein VC0071 asnC NP_229730 VcCD00019085 FLH199133.01F DQ772827 transcriptional regulator AsnC VC0072 NP_229731 VcCD00061275 FLH236883.01F DQ899328 sensory box-GGDEF family protein VC0073 NP_229732 VcCD00019070 FLH199114.01F DQ772828 conserved hypothetical protein VC0074 NP_229733 VcCD00019078 FLH199123.01F DQ772829 hypothetical protein VC0075 madN NP_229734 VcCD00019072 FLH199116.01F DQ772830 MadN protein VC0076 uspA NP_229735 VcCD00019083 FLH199131.01F DQ772831 universal stress protein A VC0077 NP_229736 VcCD00019038 FLH199074.01F DQ772832 hypothetical protein VC0078 ftn NP_229737 VcCD00019098 FLH199149.01F DQ772833 ferritin VC0079 NP_229738 VcCD00019066 FLH199109.01F DQ772834 conserved hypothetical protein VC0080 NP_229739 VcCD00019084 FLH199132.01F DQ772835 conserved hypothetical protein VC0081 NP_229740 VcCD00019074 FLH199118.01F DQ772836 conserved hypothetical protein VC0082 NP_229741 VcCD00019095 FLH199146.01F DQ772837 conserved hypothetical protein VC0083 ubiE NP_229742 VcCD00019056 FLH199098.01F DQ772838 ubiquinone-menaquinone biosynthesis methlytransferase UbiE VC0084 NP_229743 VcCD00019109 FLH199163.01F DQ772839 conserved hypothetical protein VC0085 NP_229744 VcCD00017414 FLH174511.01F DQ772840 ubiquinone biosynthesis protein AarF, putative VC0086 tatA-1 NP_229745 VcCD00019055 FLH199097.01F DQ772841 tatA protein VC0087 NP_229746 VcCD00061117 FLH234778.01F DQ899329 conserved hypothetical protein VC0088 NP_229747 VcCD00019050 FLH199092.01F DQ772842 conserved hypothetical protein VC0089 yhjA NP_229748 VcCD00061097 FLH234727.01F DQ899330 cytochrome c551 peroxidase VC0090 dinF NP_229749 VcCD00025645 FLH214558.01F DQ772843 DNA-damage-inducible protein F VC0091 NP_229750 VcCD00019065 FLH199108.01F DQ772844 O-methyltransferase-related protein VC0092 lexA NP_229751 VcCD00019039 FLH199075.01F DQ772845 LexA repressor VC0093 plsB NP_229752 VcCD00020057 FLH200645.01F DQ772846 glycerol-3-phosphate acyltransferase VC0094 ubiA NP_229753 VcCD00019061 FLH199104.01F DQ772847 4-hydroxybenzoate octaprenyltransferase VC0095 ubiC NP_229754 VcCD00025647 FLH214567.01F DQ772848 chorismate--pyruvate lyase VC0096 NP_229755 VcCD00060989 FLH234246.01F DQ899331 hypothetical protein VC0097 NP_229756 VcCD00019080 FLH199127.01F DQ772849 flagellar protein FliL, putative VC0098 NP_229757 VcCD00019111 FLH199166.01F DQ772850 methyl-accepting chemotaxis protein VC0099 glpG NP_229758 VcCD00019059 FLH199102.01F DQ772851 glpG protein VC0100 glpE NP_229759 VcCD00017732 FLH175972.01F DQ772852 glpE protein VC0101 NP_229760 VcCD00017728 FLH175948.01F DQ772853 hypothetical protein VC0102 NP_229761 VcCD00017718 FLH175892.01F DQ772854 hypothetical protein VC0103 NP_229762 VcCD00017711 FLH175848.01F DQ772855 conserved hypothetical protein VC0104 NP_229763 VcCD00025502 FLH175862.01F DQ772856 hypothetical protein VC0105 hemB NP_229764 VcCD00025461 FLH174670.01F DQ772857 delta-aminolevulinic acid dehydratase VC0106 NP_229765 VcCD00017744 FLH176016.01F DQ772858 conserved hypothetical protein VC0107 NP_229766 VcCD00025504 FLH175871.01F DQ772859 hypothetical protein VC0108 polI NP_229767 VcCD00061284 FLH236919.01F DQ899332 DNA polymerase I VC0109 NP_229768 VcCD00017707 FLH175828.01F DQ772860 hypothetical protein VC0110 NP_229769 VcCD00017712 FLH175852.01F DQ772861 hypothetical protein VC0111 NP_229770 VcCD00017765 FLH176109.01F DQ772862 conserved hypothetical protein VC0112 cycA NP_229771 VcCD00017770 FLH176131.01F DQ772863 cytochrome c4 VC0113 NP_229772 VcCD00017769 FLH176125.01F DQ772864 methyltransferase-related protein VC0114 NP_229773 VcCD00017755 FLH176071.01F