Timothy Colston Biological Science Harnessing NGS Technologies to Understand Biological Diversification: From Microbes to Macroevolutionary Patterns [email protected] Biodiversity @maddreptiles Source: International Conference on Biodiversity Motivation & Tools –Molecular [email protected] @maddreptiles (NGS) [email protected] Biodiversity @maddreptiles Source: International Conference on Biodiversity [email protected] Biodiversity @maddreptiles [email protected] Biodiversity –the “microbiome” @maddreptiles NGS Sequencing [email protected] Biodiversity –the “microbiome” @maddreptiles NGS Sequencing [email protected] Biodiversity –the “microbiome” @maddreptiles • Plants and Animals are “metagenomic organisms” – Co‐evolution • Host‐associated microbial cells ~ 10X number of host cells – Fitness/Selection – Heritable by Gaby D'Allesandro / © AMNH [email protected] Biodiversity –the “microbiome” @maddreptiles • Plants and Animals are “metagenomic organisms” – Co‐evolution • Host‐associated microbial genes > 10X number of host cells – Fitness/Selection – Heritable by Gaby D'Allesandro / © AMNH [email protected] Biodiversity –the “microbiome” @maddreptiles Mammals Fish Birds Amphibians Reptiles Colston, T.J. & Jackson, C.R. (2016) Molecular Ecology The Reptile Microbiome C h a m A a g e a A l m e m V o L i a p d n a h a i r H d a n i s e e L t T a n b h S l a r a i A o e A d o a c h X e d n g n a n e i n D e T e o n g r e o n i n t n r r a d i u i L t i s m o o e d o c i a e i d a p p a l s t d e i a y l h o i e a u d a i a l d t C c i B o r u i u e i e e d d o i a l i r r d a p d a a r i i e n y d a h d e a Cadeidae e t i a a d Crotaphytidaeo a e e P p li e e a e h d e e a h a a a id ry e id m n n d al o id e h s a p ht P o e i p o m B no ly a m H ch ti ae y o r d id G p ot ae r lo id eu ce ae in rc h e Ig id R da ua ae eii nid T ae Op lur ida ae Le e cid iosa cin urida S lidae e ordy Liola C dae emida osauri Lept e Gerrh otyphlopidae Xantusiidae Gerrhopilidae Gekkonidae idae Phy Xenotyphlop llodactylidae S phaero dactyli idae Eub dae yphlop leph T S arid ae p ae pidid h D male en iplo Ano ae o dac iliid do D Py tyl An n ib go ida ti a C po e e da m a di da e id rp da hii ae ho e op da pid e ct ro da yl T rii ida ba e e la a a iid C in e nz a a id S n ri a h E C r y c Colubridae B i d *390 o C a [email protected] i d e y a Candoiidae l E i e n A la d L n p X r a o i U o H m d e m L p a r o P n p e o h o o B X m o r y V i p x o c i p t o e a d A i e o p h h P p e l l a n X h l o c i y c e o t a l e l o p i i i e e t r e r i individuals, n r d d i d p o e i s m d r n d i a a a i i d h c a a i o a d e e e i d h a i t d a e d e d i @maddreptiles a d e o e a e i e a i r e r d d m e a i d e a a t i e d a e 22 squamate families, ~150 spp [email protected] Reptile Tree of Life @maddreptiles Tonini et al. 2016 [email protected] Reptile Tree of Life – NGS Solutions @maddreptiles • Museum collections • Next generation phylogenetics • 1000’s of genes tcolston@fsu.