Authors: Pallavi Subhraveti Ron Caspi Peter Midford Peter D Karp An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Ingrid Keseler Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. Gcf_000716605Cyc: Streptomyces sp. NRRL F-525 NRRL F-525 Cellular Overview Connections between pathways are omitted for legibility. Anamika Kothari an amino an amino an amino an amino an amino an amino an amino an amino phosphate phosphate acid acid acid acid acid acid acid acid molybdate an amino an amino H + a dipeptide phosphate phosphate phosphate acid an amino acid an amino H + phosphate phosphate glycine betaine glycine betaine glycine betaine leu pro pro pro acid acid predicted predicted predicted predicted predicted predicted predicted predicted predicted predicted predicted predicted ABC ABC ABC ABC ABC ABC ABC ABC ABC ABC ABC F0F1 ATP ABC RS16420 RS10685 RS06340 RS46495 RS16425 ProP RS28920 RS26410 LeuE RS27350 RS38790 RS13800 transporter transporter transporter RS41005 transporter transporter transporter transporter transporter CoxB transporter transporter transporter synthase transporter of an of an of an of an of an of an of an of an of molybdate of phosphate of phosphate of a dipeptide amino acid amino acid amino acid amino acid amino acid amino acid amino acid amino acid a sugar RS40790 a sugar an amino an amino H + phosphate phosphate phosphate glycine betaine glycine betaine glycine betaine an amino an amino H + phosphate phosphate leu acid acid pro pro pro acid acid molybdate a sugar RS33225 a sugar phosphate phosphate an amino an amino an amino an amino an amino an amino an amino an amino a dipeptide acid acid acid acid acid acid acid acid a sugar RS17620 a sugar Amino Acid Degradation Chlorinated Degradation/ Compound Degradation a sugar RS17775 a sugar 5-methoxy-6- ditrans,octacis- a DNA containing 2-hydroxy-4-oxobutane- a [bis(guanylyl UDP-N-acetyl- a [protein]- (S)-4-hydroxy- (R)-4-hydroxy- 4-hydroxy- a [protein]-L- Utilization/ + serotonin a ribonucleoside 4-hydroxy- Ala mycofactocin biosynthesis Cofactor, Carrier, and Vitamin Biosynthesis Fatty Acid and Lipid Biosynthesis L-valine degradation I NaMN adenosylcobinamide a purine undecaprenyldiphospho- menadione NADH H an apurinic/ molybdopterin) α a glycyl-[tRNA ] 4-methyl-2- 4-methyl-2- cob(II)yrinate a,c-diamide [2Fe-2S] iron-sulfur mycothiol biosynthesis adeninyl adenosylcobamide biosynthesis Fermentation (S)-lactate fermentation to propanoate, acetate and hydrogen L-isoleucine degradation I L-threonine degradation I L-arginine degradation V L-leucine degradation I L-histidine degradation II 1,2-dichloroethane methylbenzimidazole 1,2,4-tricarboxylate cys -D-muramoyl- L-glutamine 2-oxopentanoate diphosphate dGDP methionine cob(II)yrinate a,c- superpathway of coenzyme A biosynthesis I (bacteria) flavin biosynthesis I (bacteria and plants) molybdenum cofactor biosynthesis L-arginine degradation XIII Assimilation - Other ribonucleoside β L-threonine oxoglutarate peptide- (arginine deiminase pathway) degradation N-acetyl-(N-acetyl- -D- apyrimidinic site cofactor L-alanyl-γ-D- ribonucleotide- oxoglutarate a C-terminal diamide biosynthesis I biosynthesis II (late cluster biosynthesis from adenosylcobinamide-GDP (reductive Stickland reaction) acrylonitrile 5'-triphosphate 4-carboxy-4- 4-carboxy-4- ribonucleotide- D-glucopyranose palmitate biosynthesis glucosaminyl)muramoyl- chaperone]- glutamyl-meso-2,6- diphosphate methionine a sugar RS17895 a sugar [mycofactocin cobalt incorporation) 3-methyl-2- a 5,10- fatty acid elongation -- saturated (S)-lactate val degradation I nicotinate-nucleotide-- 4-carboxy-4-hydroxy- transglutaminase 4-hydroxy-2- hydroxy-2- hydroxy-2- diphosphate (early cobalt insertion) 6-phosphate molybdate (type II fatty acid synthase) ile thr leu his 1,2-dichloroethane D-aspartate L-alanyl-γ-D-glutamyl-L- NADH-quinone L-cysteine diaminopimeloyl- (S)-S-oxide oxobutanoate methylenetetrahydrofolate endonuclease oxovalerate reductase: low specificity D-tyrosyl- oxoadipate oxoadipate precursor uroporphyrinogen-III L-cysteine desulfurase GTP arg arg histidine haloalkane dimethylbenzimidazole bifunctional lysyl-D-alanyl-D-alanine oxidoreductase: 2-oxoadipate domain-containing reductase: reductase uroporphyrinogen-III inositol-3- adenine threonine/serine branched-chain III: nth D-alanyl-D-alanine OO69_RS19920 L-threonine tRNA(Tyr) aldolase/ aldolase/ peptide]-glycyl-L-mycofactocin bifunctional predicted branched-chain branched-chain arginine deiminase: ammonia-lyase: acrylonitrile dehalogenase: phosphoribosyltransferase: adenosylcobinamide OO69_RS22360 aldolase/oxaloacetate protein: aldolase: dmpG OO69_RS19920 MsrA: msrA bifunctional 3-methyl-2-oxobutanoate phosphate molybdopterin an acetoacetyl-[acp] dehydratase: amino acid OO69_RS19925 aldolase: deacylase: valyl-L-tyrosine cysteine superpathway of thiamine diphosphate biosynthesis II ABC an acetoacetyl-[acp] amino acid arginine OO69_RS12995 nitrile hydratase cobT kinase/ bifunctional DNA- decarboxylase: cysteine OO69_RS22155 4-hydroxy-2- oxaloacetate oxaloacetate OO69_RS19925 a sugar RS25090 a sugar radical SAM uroporphyrinogen-III uroporphyrinogen-III GTP nicotinate-nucleotide-- amino acid OO69_RS13530 aminotransferase: OO69_RS00800 OO69_RS38555 OO69_RS22370 OO69_RS16130 peptide- a C-terminal desulfurase: hydroxymethyltransferase: synthase: aminotransferase: deiminase: subunit adenosylcobinamide- D-aspartate formamidopyrimidine OO69_RS29765 desulfurase: D-alanyl-D-alanine vitamin B12- OO69_RS01030 decarboxylase: decarboxylase: maturase: mftC C-methyltransferase/ cyclohydrolase II: transporter molybdopterin aminotransferase: arginine deiminase: [+ 2 isozymes] 2-chloroethanol nicotinate-nucleotide-- OO69_RS22375 nuoE transglutaminase oxovalerate vitamin B12- [mycofactocin C-methyltransferase/ OO69_RS23835 panB OO69_RS39400 dimethylbenzimidazole 3-oxoacyl- pyruvate OO69_RS23605 threonine OO69_RS12995 4-methyl-2-OO69_RS23605 ligase: glycosylase/ OO69_RS25690 carboxypeptidase/ dependent low specificity OO69_RS29765 OO69_RS29765 methionine uroporphyrinogen- OO69_RS36240 a [ThiS sulfur- of molybdate: adenylyltransferase: OO69_RS23605 OO69_RS35915 urocanate alpha: nthA dimethylbenzimidazole phosphate nuoF OO69_RS22390 4-carboxy-4-hydroxy- domain-containing aldolase: dmpG precursor peptide]- uroporphyrinogen- cys phosphoribosyltransferase: ACP ammonia-lyase: oxopentanoate acrylamide OO69_RS22450 DNA-(apurinic D-alanyl-D-alanine- ribonucleotide L-threonine dependent (S)-S-oxide III synthase: [+ 3 isozymes] [+ 2 isozymes] carrier protein] modA modB OO69_RS38940 3-methyl-2- arginine L-citrulline phosphoribosyltransferase: guanylyltransferase: nuoH nuoI 2-oxoadipate [+ 3 isozymes] protein: 4-carboxy-4- 4-carboxy-4- glycyl-L-valyl- III synthase: Ins(3)P UDP-N-acetyl- cobT reductase (3S)-3-methyl-2- OO69_RS36720 a tRNA Ala ribonucleotide reductase predicted 2,5-diamino-6- pyruvate carboxylase: oxobutanoate (2Z)-2-aminobut- deiminase: urocanate OO69_RS31010 OO69_RS22405 nuoK or apyrimidinic endopeptidase: dacB reductase: aldolase: gly OO69_RS21795 OO69_RS21795 an S-sulfanyl-[L- 2-dehydropantoate α-D-glucosamine sulfur carrier cysteine molybdopterin FabG: fabG oxopentanoate amidase: cobT aldolase/oxaloacetate OO69_RS32525 hydroxy-2- hydroxy-2- reductase: MsrA: msrA ABC decarboxylated (5-phospho-D- nicotinate-nucleotide-- molybdate 2-enoate OO69_RS35915 ornithine hydratase: ditrans,octacis- site) lyase: mutM a [bis(guanylyl [+ 2 isozymes] acetaldehyde pyruvate OO69_RS41840 OO69_RS14125 a protein-L- a sugar a sugar uroporphyrinogen-III cysteine desulfurase] protein ThiS desulfurase: D-glyceraldehyde adenine dinucleotide OO69_RS07855 adenosyl- OO69_RS22420 nuoN decarboxylase: a 2'- oxoadipate oxoadipate OO69_RS41840 transporter L-tyrosine uroporphyrinogen-III 2-dehydropantoate thioredoxin ribosylamino) dimethylbenzimidazole 3-oxoacyl- a 3-oxoacyl-[acp] 3-oxoacyl- carbamoyltransferase: OO69_RS32145 OO69_RS04570 chloroacetaldehyde undecaprenyldiphospho-N- ala molybdopterin) methionine- mycofactocin D-inositol-3-phosphate adenylyltransferase: OO69_RS23835 3-phosphate 5-methoxy-6- cobinamide NADH-quinone OO69_RS24400 a [protein] N 6 - deoxyribonucleoside aldolase/ aldolase/ of a sugar C-methyltransferase: C-methyltransferase: asp pathway phosphoribosyltransferase: [acyl-carrier- [acyl-carrier- L-citrulline 3-methylbutanoyl- argF [+ 6 isozymes] acetyl-(N-acetylglucosaminyl) GDP radical SAM 2-reductase: pyrimidin- glycosyltransferase: 4-imidazolone- nicotinate phosphate a purine cofactor UDP-N- 5'-diphosphate oxaloacetate oxaloacetate (S)-S-oxide cobA moeZ [+ 3 isozymes] 1-deoxy-D- molybdopterin protein] oxaloacetate formate acetyl-CoA CoA aldehyde methylbenzimidazole γ oxidoreductase a 3'-terminal a 5'-phospho- [(5-hydroxy-1H- glycolaldehyde cobA OO69_RS05705 4(3H)-one cobT protein] (2S)-2- 5-propanoate ribonucleoside muramoyl-L-alanyl- -D- chaperone]-S- D-ala acetylmuramoyl- gly maturase: mftC precorrin-1 aspartate 1- an oxidized mshA a carboxy- an [L-cysteine xylulose-5- molybdotransferase reductase: isobutanoyl-CoA acrylate dehydrogenase ribotide phosphate subunit NuoK: nuoK unsaturated sugar [DNA] indol-3-yl)ethyl]- decarboxylase: decarboxylase: predicted precorrin-1 reductase: methylbutanoyl-