446 Persoonia – Volume 44, 2020 Tuber alcaracense Fungal Planet description sheets 447 Fungal Planet 1107 – 29 June 2020 Tuber alcaracense Ant. Rodr. & Morte, sp. nov. Etymology. Referring to Alcaraz mountain range, where the type speci- Typus. SPAIN, Albacete, Peñascosa, in calcareus soil, in Quercus ilex men was collected. subsp. ballota (Fagaceae) forest, 15 Feb. 2017, A. Rodríguez (holotype MUB Fung-971; ITS and LSU sequences GenBank MN810047 and MN953777, Classification — Tuberaceae, Pezizales, Pezizomycetes. MycoBank MB833685). Ascomata hypogeous, 1–4 cm, subglobose, covered with Additional material examined. SPAIN, Albacete, Vianos, in Quercus ilex brown-black pyramidal warts, 4–6-sided, 2–3(–4) mm across, subsp. ballota forest, 11 Jan. 2015, A. Rodríguez, MUB Fung-928; ITS sequence GenBank MN810046. 1–4 mm high, often depressed at the apex. Peridium 150–250 μm thick, pseudoparenchymatous, composed of subglobose, Notes — Tuber alcaracense is a black truffle of the aestivum angular cells, 10–20 μm diam, pale yellow and thin-walled in clade characterised by its brown-black warty peridium, brown the innermost layers, dark red-brown and with thicker walls in gleba marbled with thin white veins and reticulate-alveolate the outermost layers. Gleba firm, solid, white when immature, spores. It resembles Tuber mesentericum, but in addition to becoming dark brown at maturity, marbled with numerous, genetic differences it differs from T. mesentericum (Vittadini thin, white, meandering veins that do not change colour when 1831) by having a pleasant odour and lacking a basal cavity. exposed to the air. Pleasant odour. Asci inamyloid, 60–90 × 50–75 μm, walls thickened, 1–2 μm, ellipsoid to subglobose, with a short stalk, 10–35 × 5–7 μm, (1–)3–4(–5)-spored. Ascospores 26–47 × 22–37 μm, Q = 1.1–1.4, excluding orna- mentation, yellowish, ellipsoid to subglobose, ornamented with a coarse irregular reticulum, 3–5 µm high, sometimes bending at the top. Meshes variable, usually 3–5 across width of spore and often with incomplete secondary crests inside. Ascospores from 1-spored asci 45–47 × 35–37 μm, 2-spored asci 38–41 × 30–35 μm, 3-spored asci 32–35 × 25–30 μm, 4-spored asci MN962726 99 28–33 × 23–27 μm and 5-spored asci 26–30 × 22–23 μm. Tuber aestivum MN962727 Ecology & Distribution — Tuber alcaracense grows in Medi- 97 terranean Quercus ilex subsp. ballota forest, in limestone moun- KY081688 Tuber sinoaestivum 99 tains of the southeast of the Iberian Peninsula, 1 000–1 400 m JN896355 alt., from December to February. MK113982 99 Tuber pulchrosporum MK113980 MN962725 Tuber panniferum 100 HM485380 Tuber sp. KP686238 100 MN810246 100 Tuber alcaracense sp. nov. 100 MN810247 holotype AF516798 100 94 KY660021 81 AF516794 JQ348414 Tuber mesentericum 85 100 AF516793 MN962728 100 KP686246 MN962723 Tuber malenconii 100 MN962724 FM205629 Tuber magnatum 100 JQ925645 Tuber macrosporum AF106885 0.20 Maximum likelihood (ML) phylogenetic tree inferred from ITS sequences, using RAxML-HPC v. 8 (Stamatakis 2014) on XSEDE in the CIPRES science gateway (Miller et al. 2010). GTR + G selected as model of evolution for analysis. The sequences obtained in the present study are highlighted in Colour illustrations. Spain, Alcaraz mountain range (Albacete), Mediter- bold. Bootstrap support values (≥ 70 %) are indicated at the nodes. Tuber ranean Quercus ilex subsp. ballota forest. Ascocarps; mature ascospores. macrosporum AF106885 was used as outgroup. The scale bar indicates the Scale bar = 20 μm. expected changes per site. Antonio Rodríguez, Alfonso Navarro-Ródenas, Francisco Arenas, Angel Luigi Guarnizo & Asunción Morte, Departamento de Biología Vegetal (Botánica), Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain; e-mail: [email protected], [email protected], [email protected], [email protected] & [email protected] © 2020 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute.