Regulation of Host Translational Machinery by African Swine Fever Virus Alfredo Castello´ ¤, Ana Quintas, Elena G. Sa´nchez, Prado Sabina, Marisa Nogal, Luis Carrasco, Yolanda Revilla* Centro de Biologı´a Molecular Severo Ochoa, CSIC-UAM, Universidad Auto´noma de Madrid, Madrid, Spain Abstract African swine fever virus (ASFV), like other complex DNA viruses, deploys a variety of strategies to evade the host’s defence systems, such as inflammatory and immune responses and cell death. Here, we analyse the modifications in the translational machinery induced by ASFV. During ASFV infection, eIF4G and eIF4E are phosphorylated (Ser1108 and Ser209, respectively), whereas 4E-BP1 is hyperphosphorylated at early times post infection and hypophosphorylated after 18 h. Indeed, a potent increase in eIF4F assembly is observed in ASFV-infected cells, which is prevented by rapamycin treatment. Phosphorylation of eIF4E, eIF4GI and 4E-BP1 is important to enhance viral protein production, but is not essential for ASFV infection as observed in rapamycin- or CGP57380-treated cells. Nevertheless, eIF4F components are indispensable for ASFV protein synthesis and virus spread, since eIF4E or eIF4G depletion in COS-7 or Vero cells strongly prevents accumulation of viral proteins and decreases virus titre. In addition, eIF4F is not only activated but also redistributed within the viral factories at early times of infection, while eIF4G and eIF4E are surrounding these areas at late times. In fact, other components of translational machinery such as eIF2a, eIF3b, eIF4E, eEF2 and ribosomal P protein are enriched in areas surrounding ASFV factories. Notably, the mitochondrial network is polarized in ASFV-infected cells co-localizing with ribosomes. Thus, translation and ATP synthesis seem to be coupled and compartmentalized at the periphery of viral factories. At later times after ASFV infection, polyadenylated mRNAs disappear from the cytoplasm of Vero cells, except within the viral factories. The distribution of these pools of mRNAs is similar to the localization of viral late mRNAs. Therefore, degradation of cellular polyadenylated mRNAs and recruitment of the translation machinery to viral factories may contribute to the inhibition of host protein synthesis, facilitating ASFV protein production in infected cells. Citation: Castello´ A, Quintas A, Sa´nchez EG, Sabina P, Nogal M, et al. (2009) Regulation of Host Translational Machinery by African Swine Fever Virus. PLoS Pathog 5(8): e1000562. doi:10.1371/journal.ppat.1000562 Editor: Ian Mohr, New York University, United States of America Received March 31, 2009; Accepted July 31, 2009; Published August 28, 2009 Copyright: ß 2009 Castello´ et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by Grants from Laboratorios Esteve, Ministerio de Educacio´n y Ciencia BFU2007-63110 and BFU2006-02182, and by an Institutional grant from the Fundacio´n Ramo´n Areces. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: [email protected] ¤ Current address: European Molecular Biology Laboratory (EMBL), Heidelberg, Germany Introduction eukaryotic cells by means of phosphorylation processes [7,8]. In example, Pak-2 phosphorylate eIF4GI at the eIF4E-binding site, The vast majority of animal cytolytic viruses interfere with inhibiting the interaction between both factors [7]. On the other cellular gene expression after infection of host cells. Cellular hand, eIF4E is phosphorylated by the protein kinase Mnk-1, protein synthesis in particular is usually abrogated at times when although the role of this event is still under investigation [2,9]. In late viral proteins are being synthesized [1–3]. However, the addition, there are several eIF4E-binding proteins (4E-BPs) that molecular mechanisms by which viruses induce this phenomenon have the ability to sequester eIF4E in a phosphorylation- are still under investigation. Eukaryotic initiation factor (eIF) 4F is dependent manner [9,10]. In their hypophosphorylated form, composed of eIF4E, eIF4A and eIF4G (Figure 1A). eIF4E binds 4E-BPs inhibit cap-dependent translation on interacting with the cap structure present at the 59 end of cellular mRNAs; eIF4A is eIF4E. In contrast, hyperphosphorylation of 4E-BPs by mTOR an RNA helicase that unwinds the secondary structure near to the kinase leads to their release from eIF4E and subsequent initiation codon and eIF4G is a scaffolding protein that physically association with eIF4G, thereby assembling an active eIF4F links the mRNA and the small ribosomal subunit by means of complex [9,10]. several protein-protein interactions [4]. In particular, the N- Given the essential role of eIF4F in cellular mRNA translation, terminal domain of eIF4G binds to eIF4E and poly(A)-binding it is not surprising that many animal viruses target eIF4F during protein (PABP), which are involved in mRNA recruitment through the viral cycle [2,3]. This is the case of some picornaviruses, interaction with the cap and the poly(A) tail, respectively (scheme retroviruses and caliciviruses, which encode proteases that cleave in Figure 1A) [5,6]. The C-terminal domain of eIF4G interacts eIF4G, separating its N-terminal and C-terminal domains [11]. with eIF4A, mitogen-activated kinase 1 (Mnk-1) and eIF3, a Other viruses such as encephalomyocarditis virus (EMCV), complex eIF that binds the 40S ribosomal subunit [4]. In addition, adenoviruses (AdV) or vesicular stomatitis virus (VSV) induce eIF4F functions are regulated by extra- and intracellular signals in the dephosphorylation of eIF4E and 4E-BPs, leading to inactiva- PLoS Pathogens | www.plospathogens.org 1 August 2009 | Volume 5 | Issue 8 | e1000562 Control of Protein Synthesis by ASFV Author Summary fully dependent on the cellular translational machinery to synthesize viral proteins. In the present work, a number of eIFs African Swine Fever Virus (ASFV) is a large DNA virus that are analyzed in ASFV-infected cells. We provide evidence that infects different species of swine, causing an acute, highly eIF4E and eIF4G are phosphorylated at Ser209 and Ser1108 contagious and often fatal disease. Infection by ASFV is respectively and these events strongly correlate with a robust viral characterized by the absence of a neutralizing immune protein synthesis and an increase of eIF4F assembly. Inhibition of response, which has so far hampered the development of either eIF4GI or eIF4E phosphorylation partially affects viral a conventional vaccine. While a number of reports have protein synthesis and virus spread, whereas the knock down of been concerned with ASFV genes and mechanisms these factors strongly avoid ASFV infection. On the other hand, regulating programmed cell death and immune evasion, eIF4GI and eIF4E are recruited within ASFV factories at 8 hpi, nothing is known so far regarding how ASFV replicates in and they are later redistributed to the periphery of these particular the infected cells. As intracellular parasites, viruses are foci. Finally, eIF4GI, eIF4E, eIF2a, eIF3b, eEF2 and ribosomes highly dependent on host translation machinery for synthesizing their own proteins. We have observed that are closely distributed to ASFV factories at 16 hpi, being ASFV the cellular protein synthesis is strongly inhibited during late mRNAs and mitochondrial network found at these areas. ASFV infection, while viral proteins are efficiently pro- duced. Furthermore, we here describe the processes by Materials and Methods which ASFV activates and redistributes the cellular machinery to synthesize its own proteins. It has been Cell culture, viruses, and reagents reported that ASFV replicates within discrete cytoplasmic Vero and COS-7 (African green monkey kidney) cells were areas known as factories. In this regard, we have identified obtained from the American Type Culture Collection (ATCC) and the presence of important cellular factors involved in the grown in Dulbecco’s Modified Eagle’s Medium supplemented with control of protein synthesis, located close to viral factories, 5% fetal bovine serum (Invitrogen Life Technologies). Cells were together with ribosomes and the mitochondrial network, grown at 37uC under a 7% CO2 atmosphere saturated with water which represents a sophisticated mechanism of viral vapor in a culture medium supplemented with 2 mM L-glutamine, control. 100 U/ml gentamicin and nonessential amino acids. The Vero- adapted ASFV strain Ba71V was propagated and titrated by plaque tion of cap-dependent translation [12–15]. By contrast, mRNAs assay on Vero cells, as described [26–29]. Infection of Vero and synthesized by some DNA viruses, such as herpes simplex virus COS-7 cells with Ba71V ASFV was carried out at a multiplicity of type 1 (HSV-1), human citomegalovirus (HCMV) or vaccinia virus 1–5 pfu/cell. Silencing was achieved by transfecting COS-7 or (VV), are translated by a cap-dependent mechanism. These viruses Vero cells twice (0 and 24 h) with 25 nM siRNAs (siControl [Gene can in fact stimulate eIF4F assembly to enhance viral protein Link], si4E [TACATTAATCGGTAGCAGGAA] [32], si4GII-2 synthesis [16–19]. In addition to eIF4F, the phosphorylation of [CAAAGACCTGGACTTTGAA] (EW, AC, PM and LC, unpub- eIF2a may play a key
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