SEAN J. ELLIOTT Department of Chemistry - Boston University 590 Commonwealth Ave. - Boston, MA 02215 - (617) 358-2816 - [email protected] Updated 14 November 2013 Research Interests My research is focused on the redox chemistry of bioinorganic proteins and enzymes: multi- electron catalysts, enzymes, and complex metalloproteins such as multi-heme cytochromes c. For the past decade, I have specialized in the use of protein electrochemistry as a means of monitoring biological electron transfer processes coupled to enzymatic chemistry and long- range, extra-cellular ET. My work has spanned the problems of redox reactivity and mechanistic chemistry of peroxidase enzymes, redox regulation and lability of novel [Fe2S2] proteins, and the mechanisms of extra-cellular ET in Shewanella. I have demonstrated the use of protein electrochemistry as a means to probe drug binding at redox proteins, and developing new electrochemical techniques for examining protein-protein interactions. Education 1990-1994 B.A. in Chemistry and English Amherst College summa cum laude, Phi Beta Kappa 1993 Bioinorganic Chemistry University of East Anglia Study Abroad, Norwich, UK 1994-2000 Ph.D. Bioinorganic Chemistry Caltech Division of Chemistry and Chemical Engineering Pasadena, CA Thesis Advisor: Professor Sunney I. Chan 2000 – 2002 EMBO Post-doctoral Fellow University of Oxford Inorganic Chemistry Laboratory Oxford, UK Advisor: Professor Fraser Armstrong, F.R.S. Academic Experience 2002 – 2008 Assistant Professor Boston University Dept. of Chemistry Molecular Biology, Cell Biology & Biochemistry Program 2008 – present Associate Professor with tenure Boston University Dept. of Chemistry, Molecular Biology, Cell Biology & Biochemistry Program Fall 2011 Visiting Associate with Prof. Anthony Sinskey MIT Department of Biology Cambridge, MA Spring 2012 Visiting Associate with Prof. Dianne Newman Caltech Division of Biology Pasadena, CA Sean J. Elliott! Curriculum vitae !Page 1 Honors and Awards 2011, 2013 Research Corporation for Science Collaborative Innovation Awardee 2010 Research Corporation Scialog Awardee 2008 Templeton Award for Excellence in Advising, Boston University 2007 Gitner Award for Distinguished Teaching, Boston University 2006 NSF CAREER Award 2005 – 2007 Smith Family Young Investigator Award 2000 – 2002 EMBO Long-term Postdoctoral Fellow 1997 – 2000 National Research Service Award (NIH) 1998 – 1999 Forris Jewett Moore Fellowship 1994 Doughty Award for Chemistry 1994 Phi Beta Kappa Student Honors Resulting from Laboratory Research ! 2014 Stephanie Maiocco, Selected Attendee for New England American Chemical Society (NESACS) - German Chemical Society (GDCh) Exchange, Jena, Germany ! 2013 Evan T. Judd, Selected Oral Presentation, National Meeting of the Biophysical Society, Philadelphia, PA Evan T. Judd, Selected Oral Presentation, Graduate Research Seminar (GRC) in Bioinorganic Chemistry, Ventura, CA 2012 Stephanie Maiocco, Best Poster Award, Women in Chemistry Symposium @ Harvard University, Cambridge, MA Daniel W. Bak, Selected Oral Presentation, Workshop in Bioinorganic Chemistry at the Pennsylvania State University, State College, PA 2011 Kathryn D. Bewley and Katie Ellis, Selected Attendees for New England American Chemical Society (NESACS) - German Chemical Society (GDCh) Exchange, Erlangen, Germany Katie Ellis, Selected Oral Presentation, Graduate Research Seminar (GRC) in Bioinorganic Chemistry, Ventura, CA 2010 Mackenzie Firer-Sherwood, Selected Oral Presentation, Graduate Research Seminar (GRC) in Bioinorganic Chemistry, Ventura, CA 2006 Sarah Chobot (BU Undergrad) attendee for New England American Chemical Society (NESACS) - German Chemical Society (GDCh) Exchange, Humbolt, Germany Sean J. Elliott! Curriculum vitae !Page 2 Publications (undergraduate authors noted with an asterisk) From Post-Tenure Period @ BU 44. Bak DW and Elliott SJ. “Alternative FeS cluster ligands: tuning redox potentials and chemistry,” Current Opinions in Chemical Biology, 2014, 19: 50-58. http:// dx.doi.org/10.1016/j.cbpa.2013.12.015 43. Duncan GGM, Marritt SJ, Firer-Sherwood MA, Shi L, Richardson DJ, Evans SD, Elliott SJ, Butt JN, Jeuken LJC. “Protein-Protein Interaction Regulates the Direction of Catalysis and Electron Transfer in a Redox Enzyme Complex,” J. Am. Chem. Soc., 2013,135(28): 10550-6 . dx.doi.org/10.1021/ja405072z 42. Bak DW and Elliott SJ, “Conserved hydrogen bond networks tune FeS cluster binding and structural stability,” Biochemistry, 2013, 52(27): 4687-4696. dx.doi.org/10.1021/bi400540m 41. Bewley KD, Ellis KE, Firer-Sherwood MA, Elliott SJ. “Multi-heme proteins: Nature’s electronic multi-purpose tool,” Biochimica et Biophysica Acta – Bioenergetics, 2013, 1827: (8-9), 938-948. dx.doi.org/10.1016/j.bbabio.2013.03.010 40. Judd ET, Youngblut M, Pacecho AA, Elliott SJ. “Direct electrochemistry of Shewanella oneidensis cytochrome c nitrite reductase: evidence for interactions across the dimeric interface,” Biochemistry, 2012, 51(51): 10175-85. dx.doi.org/10.1021/bi3011708 39. Ellis KE, Frato KE, Elliott SJ. “Impact of Quarternary Structure upon Bacterial Cytochrome c Peroxidases: does homodimerization matter?” Biochemistry, 2012, 51(50): 10008-16. dx.doi.org/10.1021/bi301150n 38. Hamill MJ, Jost M, Wong C, Bene NC*, Drennan CL, Elliott SJ. “Electrochemical characterization of Escherichia coli adaptive response protein AidB”, International Journal of Molecular Sciences, 2012, 13(12), 16899-16915. dx.doi.org/10.3390/ijms131216899 37. Bewley KD, Firer-Sherwood MA, Mock JY*, Ando N, Drennan CL, Elliott SJ. “Mind the gap: diversity and reactivity of multiheme cytochromes of the MtrA/DmsE family,” Transactions of the Biochemical Society, 2012, 40(6), 1268-1273. dx.doi.org/10.1042/ BST20120106 36. Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan CL. “Unusual Role for a Mobile Flavin in a StaC-like Indolocarbazole Biosynthetic Enzyme,” Chemistry & Biology, 2012, 19(7), 855-865. dx.doi.org/10.1016/j.chembiol.2012.05.016 35. Seidel J, Hoffmann M, Ellis KE, Seidel A, Spatzal T, Gerhardt S, Elliott SJ, Einsle O. “MacA is a Second Cytochrome c Peroxidase of Geobacter sulfurreducens”, Biochemistry, 2012, 50(21), 4513-20. dx.doi.org/10.1021/bi300249u 34. Youngblut M, Judd EJ, Srajer V, Sayyed B, Groelzer T, Elliott SJ, Schmidt M, Pacheco AA. “Laue crystal structure of Shewanella oneidensis cytochrome c nitrite reductase from a high- yield expression system”, Journal of Biological Inorganic Chemistry, 2012, 17(4), 674-662. dx.doi.org/10.1007/s00775-012-0885-0 33. Pulcu GS, Frato KE, Gupta R, Hsu H-R,* Levin GA,* Hendrich MP, Elliott SJ. “The Cytochrome c Peroxidase from Shewanella oneidensis Requires Reductive Activation”, Biochemistry, 2012, 50(5) 974-985. dx.doi.org/10.1021/bi201135s 32. Hamill MJ, Jost M, Wong CY, Elliott SJ, Drennan CL. “Flavin Induced Oligomerization in Escherichia coli Adaptive Response Protein AidB “, Biochemistry, 2011, 49(46), 10159-10169. dx.doi.org/10.1021/bi201340t Sean J. Elliott! Curriculum vitae !Page 3 31. Levin BD, Can M, Bren KL, Elliott SJ. “Methionine Lability in Bacterial Monoheme Cytochromes c: an electrochemical study”, Journal of Physical Chemistry – B, 2011, 11718-11726. dx.doi.org/10.1021/jp203292h 30. Firer-Sherwood MA, Ando N, Drennan CL, Elliott SJ. “Solution-based Structural Analysis of the Decaheme Cytochrome, MtrA, by Small Angle X-Ray Scattering and Analytical Ultracentrifugation,” Journal of Physical Chemistry – B, 2011, 11208-11214. dx.doi.org/ 10.1021/jp203603r 29. Ellis KE, Seidel J, Einsle O, Elliott SJ. “Geobacter sulfurreducens Cytochrome c Peroxidases: electrochemical classification of catalytic mechanisms, Biochemistry, 2011, 4513-4520. dx.doi.org/10.1021/bi200399h 28. Firer-Sherwood MA, Bewley KD, Mock JY*, Elliott SJ. “ Tools for Resolving Complexity in the Electron Transfer Networks of Multiheme Cytochromes,” Metallomics 3, 2011, 344-348. dx.doi.org/10.1039/C0MT00097C 27. Cong H, Becker CF, Elliott SJ, Grinstaff MW, Porco JA. “Silver Nanoparticle-catalyzed Diels-Alder Cycloadditions of 2’-Hydroxychalcones,” J. Am. Chem. Soc., 2010, 7514-7518. dx.doi.org/10.1021/ja102482b 26. Bak DW, Zuris J, Paddock M, Jennings PA, Elliott SJ. “Redox Characterization of the FeS Protein MitoNEET and Impact of Thiazolidinedione Drug Binding”, Biochemistry, 48, 2009, 10193-95. dx.doi.org/10.1021/bi9016445 25. Becker CF, Watmough NJ, Elliott SJ. “Electrochemical Evidence for Multiple Peroxidatic Heme States of the Diheme Cytochrome c Peroxidase of Pseudomonas aeruginosa”, Biochemistry, 48(1), 2009, 87-95. dx.doi.org/10.1021/bi9016445 24. Mitra S and Elliott SJ. “Oxidative disassembly of the [2Fe-2S] Cluster of human Grx2 and Redox Regulation in the Mitochondria,” Biochemistry, 48, 2009, 3813-15. dx.doi.org/ 10.1021/bi900112m 23. Hamill MJ, Chobot SE, Hernandez HH, Drennan CL, Elliott SJ. “Direct electrochemical analyses of a thermophilic thioredoxin reductase: interplay between conformational change and redox chemistry”, Biochemistry, 47(37), 2008, 9738-46. dx.doi.org/10.1021/bi800676g 22. Hernandez HH, Jaquez OA, Hamill MJ, Elliott SJ, Drennan CL. “Thioredoxin reductase from Thermoplasma acidophilum: a new twist on redox regulation.” Biochemistry, 47(37), 2008, 9728-37. dx.doi.org/10.1021/bi8006753 21. Firer-Sherwood MA, Pulcu GS, Elliott SJ. “Electrochemical interrogations of the Mtr cytochromes from Shewanella: opening a potential window”, J. Biol. Inorg. Chem., 13(6), 2008, 849-54. dx.doi.org/10.1007/s00775-008-0398-z 20.Ye T, Kaur R, Senguen FT, Michel LV, Bren KL, Elliott SJ. “Methionine