21.10.2014USP25 antibody (pAb) Rabbit Anti -Human/M ouse Ubiquitin specific peptidase 25 (USP21) Instruction Manual Catalog Number PK-AB718-7765 Synonyms Ubiquitin specific peptidase 25, deubiquitinating enzyme 25, ubiquitin carboxyl-terminal hydrolase 25, ubiquitin thiolesterase 25, USP21, USP on chromosome 21 Description USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4). Quantity 100 µg Source / Host Rabbit Immunogen USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse Specificity USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 µg/ml. Images Available upon request. References Valero R, Marfany G, Gonzalez-Angulo O, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 1999; 62:395-405. Valero R, Bayes M, Francisca Sanchez-Font M, et al. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2001; 2: RESEARCH0043. Zhong B, Liu X, Wang X, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat. Immunol. 2012; 13:1110-7. Zhong B, Liu X, Wang X, et al. Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci. Signal. 2013; 6:ra35. Images Available upon request. Related Products Cat. No. PK-AB718-7765P - USP25 Peptide Cat. No. PK-AB718-1403 - Mouse Brain Tissue Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .