Symmetry ® Columns Applications Notebook Symmetry® SymmetryShield™ Symmetry300™ SymmetryPrep™ Intelligent Speed (IS™) www.waters.com Symmetry® Columns Setting the Standard for Reproducibility As today’s chemists establish new Unmatched Year-to-year Reproducibility analytical methods for the latest pharmaceu- ® ® tical and biopharmaceutical products, the Column: Symmetry C18, Instrumentation: Waters Alliance HT 2795 selection of a reproducible HPLC column is 4.6 x 150 mm, 5 µm Waters 996 PDA detector Mobile Phase A: H2O Sampling Rate: 5 pts./sec essential. The selected column needs to Mobile Phase B: MeCN Mobile Phase C: pH 3.75; 100 mM provide the same chromatographic results NH4COOH in H2O Flow Rate: 1.4 mL/min over the life of the new drug product. Isocratic: 30% A; 60% B; 10% C RSD’s for Retention Times Injection Volume: 5.0 µL ® 1. Terbinafine HCl 0.7% Symmetry columns demonstrate superior Temperature: 30 °C reproducibility over many years. Batches Detection: UV @ 233 nm 2. Ibuprofen 0.8% 3. Lovastatin 0.6% randomly selected over the past 5 years 4. Simvastatin 0.7% demonstrate excellent reproducibility in the example shown. Reproducibility is our number one priority in supplying Symmetry® columns. This excellent reproducibility is a result of our commitment Batch 136 (1998) to maintaining the tightest specifications in the HPLC column industry. Symmetry® columns Batch 142 (1998) are engineered with high purity raw materials, tightly controlled manufacturing Batch 149 (1999) processes and column packing procedures that provide today’s scientists with the best, Batch 151 (2000) most reproducible HPLC column available. Batch 156 (2001) Our goal is to supply a family of HPLC columns that you can rely on for rugged and Minutes robust methods. Symmetry® columns let you increase your laboratorys' productivity, and allow easier method transfer between labs and around the globe. Comprehensive Certificate of Analysis A three-page Certificate of Analysis is included with every Symmetry® column. We report both our specifications, which are the tightest in the industry, and the results of the 22 critical tests each column must pass before it carries the Symmetry® brand. Waters, Symmetry, SymmetryShield, Sentry, SymmetryPrep, Platform LC, Quattro Ultima, IS, Oasis and Alliance are trademarks of Waters Corporation. ©2003 Waters Corporation. 1 Table of Contents Introduction. 1 Compound Index . 3-4 List of Applications . 5-8 Applications . 9-182 Ordering Information . .183-186 Notes . 187-192 2 Waters, Symmetry, SymmetryShield, Sentry, SymmetryPrep, Platform LC, Quattro Ultima, IS, Oasis and Alliance are trademarks of Waters Corporation. ©2003 Waters Corporation. Compound Index Click in the box, Type desired page number, Then click OK Acephate . 5, 9 Butyl paraben . 79, 135, 150 Dichlorprop . 14 Acetaminophen . 5, 11-13 Butyrophenone . 147-148 Diclofenac Sodium . 6, 73 Acetanilide . 122-123, 147-148 Caffeine . 5, 45, 48, 140, 170 2,6-Diethylaniline . 10 Acetophenone . 147-148 Cafteric acid . 125 10,11-Dihydrocarbamezapine . 30 2-Acetylfuran . 147-148 Calcium Pantothenate . 180 Dihydroergotamine mesylate . 6, 74 Acifluorfen . 14 Captopril . 5, 46 Dilitiazem HCL . 75-76 Acyclovir . 5, 15-17 Captopril Disulfide . 46 Dinoseb . 14 Alachlor . 10 Carbadox. 5, 47 Diphenoxylate HCL . 6, 77 Albuterol . 5, 18-19 Catechins. 48 Diquat . 7, 120 Allobarbital . 33 Carbamezapine . 30 Disopyramide . 6, 78 Alpha-Tocopherol (Vitamin E) . 179 Carbamezapine-10,11-epoxide . 30 Doxepin . 6, 29, 79, 173-175 6a-Methyl-17l-hydroxyprogesterone . 122-123 Carbaryl . 36, 81 Doxycycline . 167 2-Amino-7-chloro-5-oxo-5H-[1] Cefixime . 5, 49 EDDP (Methadone Metabolite) . 99 Benzopyrano[2,3-b]pyridine-3-carbonitrile . 122-123 Cefuroxime . 5, 50 Enalapril . 80 7-Amino Clonazepam . 86 Cephalexin . 5, 51 Ephedrine . 26-27 3-Aminofluoranthene . 122-123 Chloramben . 14 Epicatechin . 48 7-Amino Flunitrazepam . 86 Chloramphenicol . 5, 52 Epigallocatechin Gallate . 48 7-Amino Nitrazepam . 86 Chlorcyclizine HCL . 65 Estazolam . 99 Amitriptyline . 173-175, 29 Chlordiazepoxide . 6, 53-54 Estradiol . 151-152 Amobarbital . 33-34 2-Chloro-2,6-diethylacetanilide . 10 Estrone . 151-152 Amoxapine. 5, 21 Chlorogenic acid . 125 Ethosuximide . 153 Amoxicillin . 5, 22-24 Chlorpheniramine . 6-7, 55, 137, 160 Etodolac . 6, 83 Amphetamine . 5, 26-27 1-(3-Chlorophenyl)peperazine . 171 Fenoprofen . 131-132 Ampicillin . 5, 25 Cholecalciferol (Vitamin D3) . 179 Flunitrazepam . 6, 85-86 Angiotensin I . 115 Cichoric acid . 125 Flurbiprofen . 131-132 Angiotensin II . 115 Cimetidine . 6, 56-58 Folic Acid . 8, 181 Antipyrine . 5, 31 Clavulanate . 23 Furazolidone . 161 Atrazine . 5, 32, 36, 81, 172 Clobetasol . 6, 59 Furosemide . 6, 88 AZT . 182 Clonazepam . 6, 60, 86 Guanosine hydrate . 17 β-hydroxyethyltheophylline (I.S.) . 45, 140 Cocaethylene . 62 Heptanophenone . 147-148 Bamethan (I.S.) . 19 Cocaine . 6, 61-62 Hexanophenone . 147-148 Barbital . 33 Codeine . 6, 11, 63-64, 139 Hydrastine . 20 Beclomethasone . 5, 35, 59 Codeine-6-Glucuronide (C-6-G) . 63-64 Hydrocodone Bitartrate . 5, 12 Benomyl . 5, 36, 81 Corticosterone . 151-152 Hydrocortisone . 6, 89, 122-123 Bentazon . 14 Cortisone . ..