<p> ISOLATION OF PHYTOCHEMICALS AND BIOLOGICAL ACTIVITY STUDIES ON The Bark of Thespesia populnea Linn.</p><p>SYNOPSIS FOR</p><p>M.PHARM DISSERTATION</p><p>SUBMITTED TO</p><p>RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES</p><p>BY</p><p>Prasoon Kumar Saxena</p><p>Department of Pharmacognosy</p><p>THE OXFORD COLLEGE OF PHARMACY</p><p>BANGALORE-78 RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES, KARNATAKA, BANGALORE ANNEXURE-II</p><p>PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION</p><p>1. Name of the candidate and Prasoon kumar saxena Address The Oxford College of Pharmacy, J.P. Nagar, Ist Phase, Bangalore-560078.</p><p>PERMANENT ADDRESS: S/o Shri Naresh kumar saxena, Shiv puram Lane no-4, Dist- Budaun, U.P. Pincode-243601.</p><p>2. Name of the Institution The Oxford College of Pharmacy, J.P.Nagar, Ist Phase, Bangalore-560078.</p><p>3. Course of study and subject M.Pharma, Pharmacognosy</p><p>4. Date of Admission 11th May 2007</p><p>5. Title of the Topic ISOLATION OF PHYTOCHEMICALS AND BIOLOGICAL ACTIVITY STUDIES ON The Bark of Thespesia populnea Linn. 6. Brief resume of the intended work:</p><p>6.1- Need for the study:</p><p>Thespesia populnea is also called as Portia tree, belonging to family</p><p>Malvaceae. It is widely distributed in India and Burma.</p><p>The plant contains glycosides such as quercetin, gossypol, and β-sitosterol1.</p><p> sesquiterpene ie. Thespesenone and dehydrooxoperezinone-6-methyl ether were isolated</p><p> from the red hard wood of Thespesia populnea2. Alanine, arginine, methionine and</p><p> tryptophan were isolated from seed of thespesia populnea3. Thespesia populnea also contain</p><p> lupenone, lupeol4. </p><p>The ethanolic extract of Thespesia populnea bark reported for anti-</p><p> inflammatory and analgesic activity5. Aqueous and methanolic extract of the Thespesia</p><p> populnea showed antioxidant activity against carbon tetrachloride induced liver injury in</p><p> rats6. Thespesia populnea also recommended for anti-fertility activity7, wound healing8,</p><p> dermatitis9, anti-bacterial and anti-fungal10, and hepatoprotective11 activity. </p><p>The more emphasis has been given to the pharmacological aspects of the</p><p> plant but a very less work has been done on the phytochemical investigation.</p><p>The aim of the present study is to carry out the isolation of</p><p> phytoconstituents and biological activity of the extract / isolated compounds. 6.2 – Review of Literature:</p><p>1. Glycoside was reported from the Thespesia populnea flower1.</p><p>2. Sesquiterpene was isolated from the red hard wood of Thespesia populnea2. </p><p>3. Amino acids were isolated from the Thespesia populnea seed3. </p><p>4. Lupenone and lupeol were isolated from the plant4.</p><p>5. The ethanolic extract of Thespesia populnea bark showed anti-inflammatory and</p><p> analgesic activity5.</p><p>6. Antioxident activity was reported from the aqueous and methanolic extract of</p><p>Thespesia populnea bark6.</p><p>7. The antifertility activity of gossypol, a phytocostituent was isolated from Thespesia</p><p> populnea bark7.</p><p>8. The aqueous extract of Thespesia populnea fruit showed wound healing activity8. </p><p>9. The plant extract was reported as dermatitis9.</p><p>10. Antibacterial and antifungal activity was reported in alcoholic and chloroform extract of</p><p>Thespesia populnea10.</p><p>11 .Methanolic and aqueous extract of Thespesia populnea bark showed hepatoprotective</p><p> activity11. 6.3 - Objective of the Study: </p><p> Collection of the authenticated plant material (i.e. bark of Thespesia populnea) from Dr. Harish Botanist. Alva’s education foundation ( R ). Alva’s Health centre complex Moodbidri – 574227. D.K.</p><p> Preparation of the extract of the drug by maceration / soxhlet method.</p><p> Isolation of chemical constituents from the extract by column chromatography / separation method.</p><p> Identification of isolated constituents by UV, IR, C13 and NMR spectroscopy.</p><p> Biological activity of the isolated compounds / extract by agar diffusion method and anthelmintic activity is studied by assay method using earth</p><p> worm.</p><p>Materials and Methods: 7. 7.1 a) Source of Data:</p><p>Search on Medline and other Journals from The Oxford College of Pharmacy, Govt. College of Pharmacy, RGUHS-Digital library.</p><p> b) Place of work: The Oxford College of Pharmacy, Bangalore. 7.2 Method of Collection of Data: </p><p> o Collection - Collection of the authenticated plant material (i.e. bark of Thespesia populnea) from Dr. Harish Botanist. Alva’s education foundation (R). Alva’s Health centre complex Moodbidri 574227. D.K. o Extraction - Extracts of the drug will be prepared using different solvents by maceration / soxhlet. o Isolation – Isolation of extracted phyto-constituents will be done using column chromatography \ Separation method. o Identification and characterization - The isolated compounds will be identified using analytical methods like UV, IR, C13 and NMR spectroscopy. o Biological activity - The isolated compounds/extract will be studied for antimicrobial activity by agar diffusion method and anthelmintic activity is studied by assay method using earth worm.</p><p>7.3 - Does the study require any investigations or inventions to be conducted on patients or other humans or animals? If so, please describe briefly.</p><p>- Not applicable -</p><p>7.4 - Has ethical clearance been obtained from your institution in case of 7.3? - Not applicable -</p><p>8. Bibliography: 1. Khare CP. Indian Medicinal plant 1st ed. Ny:Springer Internatinol Publication; 2007. 2. Puckhaber LS, Stipanovic RD. Thespesenone and dehydrooxoperezinone-6- methyl ether, new sesquterpene quinones from Thespesia populnea. J Nat Prod. 2004 Sep;67(9):1571-3. 3. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol-4 New Delhi: National Institute of Science Communication; 1995. 4. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol-5 New Delhi: National Institute of Science Communication; 1998. 5. Vasudevan M, Gunnam KK, Parle M. Antinociceptive and anti-inflammatory effect of Thespesia populnea bark extract. J Ethanopharmacol. 2007 Jan 19;109(2):264-70. 6. Havarasan R, Vasudevan M, Anbazhagan S, Venkataraman S. Antioxident activity of Thespesia populnea bark extract against carbon tetrachloride- induced liver injury in rats. J Ethanopharmacol. 2003 Aug;87(2l.-3):227-30. 7. Waller DP, Bunvapraphatsara N, Martin A, Vournazos CJ, Ahmed MS, Soejarto DD, Cordell GA, Fong HH, Russell LD, Malone JP. Effect of (+)- gossypol on fertility in male hamsters. Androl. 1983 Jul-Aug;4(4):276-9. 8. Nagappa AN, Cheriyan B. Wound healing activity of the aqueous extract of Thespesia populnea fruit. Fitoterapia. 2001 Jun;72(5):503-6. 9. Hausen BM, Knight TE, Milbrodt M. Thespesia populnea dermatitis. Am J Contact dermat. 1997 Dec;8(4):225-8. 10. Shastry CS, Aravind MB, Joshi SD, Ashok K, Bheemachari. Antibacterial and Antifungal activity of Thespesia populnea. Indian drug. 2005:42(2):81-83. 11. Havarasan R, vasuldevan M, Anbazhagan S, Venkataraman S, Sridhar SK. Hepatoprotective activity of Thespesia populnea bark extract against carbon tetrachloride-induced liver toxicity in rats. Natural product science. 2003 Jun;9(2):83-86.</p><p>9. Signature of Candidate </p><p>10. Remarks of the Guide Recommended</p><p>11. 11.1 Name and Designation of Guide S Kambhoja Professor Department of Pharmacognosy</p><p>11.2 Signature</p><p>11.3 Co-Guide _</p><p>11.4 Signature _</p><p>11.5 Head of the Department Dr. Padmaa M. Paarakh Professor and Head Department of Pharmacognosy</p><p>11.6 Signature</p><p>12. 12.1 Remarks of the Chairman and Principal Forwarded to the University for scrutiny</p><p>12.2 Signature</p><p>Dr. Padmaa M. Paarakh Principal The Oxford College of Pharmacy, J.P.Nagar, Ist Phase, Bangalore – 78.</p>