<p> PREPARATION</p><p>Ordering Information for Common Supplies</p><p>OBJECTIVE Acquire many of the supplies necessary to begin phagehunting.</p><p>HELPFUL TIPS  You may find other vendors have similar products that are perfectly acceptable. These are just the typical ones we order at Pitt.  Prices are not printed here because they are subject to change so quickly. Most vendors have pricing available on their websites and reductions for educational institutions.</p><p>VENDOR AND PRODUCT LISTS</p><p>VWR (www.vwr.com, 800.932.5000)</p><p>ThermoFisher Scientific </p><p>Sigma (www.sigma-aldrich.com, 800.325.3010) Product Catalog # Size VWR Superspin Microcentrifuge Tubes, Polypropylene 20170-038 500/pack Middlebrook 7H9 Broth Base BB11417 100g BellCo (www.bellcoglass.com, 800.257.7043) Middlebrook 7H10 Agar DF0627-17 500g Petri dishes, small, 100mm x 15 mm, standard stackable 25384-208 1 case (500 plates) New England Biolabs (www.neb.com, 800.632.5227) Petri dishes, large, 150mm x 15 mm, standard stackable 25384-139 1 case (100 plates) Syringes with BD Luer-Lock Tip, Sterile, 1 ml BD-309628 100/pack Acrodisc Sterile Syringe FiltersProduct with Supor Membrane 28143-310Catalog # 50/packSize Boric(Pall Life Acid Sciences) BP168-500 500g Calcium15ml centrifuge Chloride tube (Anhydrous) with Productplug caps, polypropylene, sterile C614-50093000-022Catalog # 500g500/caseSize 50ml centrifuge tube with plug caps, polypropylene, sterile 93000-032 500/case CarbenicillinCesium Chloride Disodium Salt (CB) C1389BP210-100 5g100g Agar, Bacto DF0140-15 100g CyclohexamideCorning Milk Dilution (CHX) Bottles, ungraduated, narrow mouth C769802-943-5 1g12/pack Beckman Coulter (www.beckmancoulter.com, 1000ml VWR Erlenmeyer flasksProduct w/ reinforced beaded top 89000-368Catalog # 6/pack Size EthidiumDextrose Bromide 10mg/ml E1510D16-500 10ml500g 2000ml50 ml shake VWR flask Erlenmeyer with baffles flasks w/ reinforced beaded top 89000-3702540-00050 4/pack-- 800.742.2345) LeucineEDTA Disodium Salt L8000BP120-500 25g500g Octagonal125 ml shake Magnetic flask with Stirbars, baffles 5/16” by 1½” 58948-1502540-00125 -- PolyethyleneEthanol, 200 proofGlycol (PEG)Product P2139-- Catalog # 500g-- Size Glycerol,250 ml shake anhydrous flask with (JT Baker)baffles JT2136-12540-00250 500ml-- Spectrum (www.spectrumchemical.com, TRIZMABamH1Magnesium restriction base Sulfate enzyme,(Anhydrous) 20,000 units/ml T1503R0136SM65-500 500g10,000 units Cla1MilliporeCostar500 mlrestriction Disposable shake Steriflip flask enzyme, DisposableStorage with baffles 5,000 Bottle, Vacuum units/ml 250ml Filter capacity Units R0197SSCGP0052528199-7582540-00500 1,00025/pack24/case-- units 800.772.8786) EcoR1(.22BD18 mmFalconµm restriction stainlesspore Polypropylene size) steel enzyme, closures Centrifuge 20,000 units/ml Tubes, 225ml R0101S21008-9432005-00018 10,00048/case-- units HaeIIISeakemVWR25 mm Vacuum restriction stainless LE Agarose Filtration steel enzyme, (Cambrex) closures Systems, 10,000 500ml, units/ml .22µm pore size, R0108SBMA5000487006-0622005-02512 3,000500g12/pack-- units Aurical Company (www.auricalcompany.com, HindIIISodiumPES38 mm Membrane, stainless Acetaterestriction Trihydrate Sterilesteel enzyme, closures 10,000 units/ml R0104SS209-32005-03812 10,0003kg-- units SodiumSpectra/Por Chloride 7 Dialysis Membranes , MWCO 1000-50000, BP358-125223-901 1kg-- 800.615.8870) Tween15.9mm 80 diameter T164-500 500ml Hydrochloric Acid (EMD) Product EM-HX0603P-5Catalog # 500ml Size USA Scientific (www.usascientific.com, HygromycinQuick-Seal Polyallomer B UltracentrifugeProduct Tubes, 13.5 ml, 80501-088342413Catalog # 1g50/box Size 800.522.8477) Corning16Albumin,Pourite x 76 mmAnti-Bubble Hoplate/Stirplate, Bovine Serum Additive (BSA), 120V Fraction V 89090-994A3611-100GM8-505 --100g60 ml</p><p>Page 1 of 2 Product Catalog # Size Screw-Cap Microcentrifuge Tubes, 1.5 ml 1415-8799 500/pack</p><p>Page 2 of 2 </p>