PREPARATION Ordering Information for Common Supplies Objective Acquire Many of the Supplies

PREPARATION

Ordering Information for Common Supplies

OBJECTIVE Acquire many of the supplies necessary to begin phagehunting.

HELPFUL TIPS  You may find other vendors have similar products that are perfectly acceptable. These are just the typical ones we order at Pitt.  Prices are not printed here because they are subject to change so quickly. Most vendors have pricing available on their websites and reductions for educational institutions.

VENDOR AND PRODUCT LISTS

VWR (www.vwr.com, 800.932.5000)

ThermoFisher Scientific

Sigma (www.sigma-aldrich.com, 800.325.3010) Product Catalog # Size VWR Superspin Microcentrifuge Tubes, Polypropylene 20170-038 500/pack Middlebrook 7H9 Broth Base BB11417 100g BellCo (www.bellcoglass.com, 800.257.7043) Middlebrook 7H10 Agar DF0627-17 500g Petri dishes, small, 100mm x 15 mm, standard stackable 25384-208 1 case (500 plates) New England Biolabs (www.neb.com, 800.632.5227) Petri dishes, large, 150mm x 15 mm, standard stackable 25384-139 1 case (100 plates) Syringes with BD Luer-Lock Tip, Sterile, 1 ml BD-309628 100/pack Acrodisc Sterile Syringe FiltersProduct with Supor Membrane 28143-310Catalog # 50/packSize Boric(Pall Life Acid Sciences) BP168-500 500g Calcium15ml centrifuge Chloride tube (Anhydrous) with Productplug caps, polypropylene, sterile C614-50093000-022Catalog # 500g500/caseSize 50ml centrifuge tube with plug caps, polypropylene, sterile 93000-032 500/case CarbenicillinCesium Chloride Disodium Salt (CB) C1389BP210-100 5g100g Agar, Bacto DF0140-15 100g CyclohexamideCorning Milk Dilution (CHX) Bottles, ungraduated, narrow mouth C769802-943-5 1g12/pack Beckman Coulter (www.beckmancoulter.com, 1000ml VWR Erlenmeyer flasksProduct w/ reinforced beaded top 89000-368Catalog # 6/pack Size EthidiumDextrose Bromide 10mg/ml E1510D16-500 10ml500g 2000ml50 ml shake VWR flask Erlenmeyer with baffles flasks w/ reinforced beaded top 89000-3702540-00050 4/pack-- 800.742.2345) LeucineEDTA Disodium Salt L8000BP120-500 25g500g Octagonal125 ml shake Magnetic flask with Stirbars, baffles 5/16” by 1½” 58948-1502540-00125 -- PolyethyleneEthanol, 200 proofGlycol (PEG)Product P2139-- Catalog # 500g-- Size Glycerol,250 ml shake anhydrous flask with (JT Baker)baffles JT2136-12540-00250 500ml-- Spectrum (www.spectrumchemical.com, TRIZMABamH1Magnesium restriction base Sulfate enzyme,(Anhydrous) 20,000 units/ml T1503R0136SM65-500 500g10,000 units Cla1MilliporeCostar500 mlrestriction Disposable shake Steriflip flask enzyme, DisposableStorage with baffles 5,000 Bottle, Vacuum units/ml 250ml Filter capacity Units R0197SSCGP0052528199-7582540-00500 1,00025/pack24/case-- units 800.772.8786) EcoR1(.22BD18 mmFalconµm restriction stainlesspore Polypropylene size) steel enzyme, closures Centrifuge 20,000 units/ml Tubes, 225ml R0101S21008-9432005-00018 10,00048/case-- units HaeIIISeakemVWR25 mm Vacuum restriction stainless LE Agarose Filtration steel enzyme, (Cambrex) closures Systems, 10,000 500ml, units/ml .22µm pore size, R0108SBMA5000487006-0622005-02512 3,000500g12/pack-- units Aurical Company (www.auricalcompany.com, HindIIISodiumPES38 mm Membrane, stainless Acetaterestriction Trihydrate Sterilesteel enzyme, closures 10,000 units/ml R0104SS209-32005-03812 10,0003kg-- units SodiumSpectra/Por Chloride 7 Dialysis Membranes , MWCO 1000-50000, BP358-125223-901 1kg-- 800.615.8870) Tween15.9mm 80 diameter T164-500 500ml Hydrochloric Acid (EMD) Product EM-HX0603P-5Catalog # 500ml Size USA Scientific (www.usascientific.com, HygromycinQuick-Seal Polyallomer B UltracentrifugeProduct Tubes, 13.5 ml, 80501-088342413Catalog # 1g50/box Size 800.522.8477) Corning16Albumin,Pourite x 76 mmAnti-Bubble Hoplate/Stirplate, Bovine Serum Additive (BSA), 120V Fraction V 89090-994A3611-100GM8-505 --100g60 ml

of 2 Product Catalog # Size Screw-Cap Microcentrifuge Tubes, 1.5 ml 1415-8799 500/pack