<p> BIOTECHNOLOGY Midyear Exam Review UNIT 1 – The Biotechnology Century and its Workforce</p><p>1. What is Biotechnology? Definition:</p><p> a. List types of biotech:</p><p> b. List some biotech jobs:</p><p>2. Scientific method: review steps (Bio text, if needed)</p><p>UNIT 2 – An Introduction to Genes and Genomes</p><p>3. cells </p><p> a. review functions of organelles (textbook pp30-31)</p><p> b. properties of water: polarity  solvent, osmosis, etc.</p><p> c. review plasma membrane – read through bellwork activity before Hepatitis C research</p><p> d. CREATE: Venn diagram that compares/contrasts eukaryotes versus prokaryotes (text p28)</p><p>PROKARYOTES EUKARYOTES 4. DNA</p><p> a. history of DNA science – review PowerPoint notes and history notes MATCHING:</p><p>1. _____ Miescher </p><p>2. _____ Griffith A. demonstrated transformation</p><p>3. _____ Avery/MacLeod/McCarty B. determined that DNA is genetic material</p><p>C. identified nucleic acids</p><p> b. DNA structure – DRAW a nucleotide (text p33)</p><p> c. LIST DNA nucleotides: ______</p><p>______</p><p> d. LIST RNA nucleotides: ______</p><p> e. What is a gene? definition: ______</p><p>5. DNA replication – explain:</p><p> a. When does this happen? ______</p><p>______</p><p> b. Where? ______</p><p>______</p><p> c. Why? ______</p><p>______</p><p> d. What molecules are involved? ______</p><p>______</p><p> e. phosphodiester bond - ______</p><p>______</p><p>6. What is a mutation? Definition: ______</p><p>______</p><p> a. LIST THREE TYPES OF MUTATIONS (text pp51-53): ______</p><p> b. What is a karyotype? Definition: ______</p><p>______</p><p>7. protein synthesis – from DNA to protein</p><p> a. Central Dogma</p><p> b. see notes from 1/6/15</p><p>8. What is the epigenome? Definition: ______</p><p>______</p><p>UNIT 3 – Recombinant DNA Technology and Genomics</p><p>9. recombinant DNA - </p><p> a. What is a restriction enzyme? Where are they found? How are they named? ______</p><p> b. What makes a good plasmid DNA vector? ______</p><p>______</p><p> c. What is transformation of bacterial cells? ______</p><p>______</p><p>10. What is cloning? ______</p><p> a. DNA libraries? ______</p><p> b. cDNA? ______</p><p>11. recombinant DNA technology</p><p> a. gel electrophoresis – How is it used? ______</p><p>______</p><p> b. DNA sequencing – How is it used? ______</p><p>______</p><p>UNIT 4 – Proteins as Products</p><p>12. LIST some enzymes (p102/text): ______</p><p>______</p><p>13. LIST some protein products (p103/text): ______</p><p>______</p><p>14. Review structures (p105/text)</p><p>15. Production – What is a bioreactor? ______</p><p>______</p><p>16. COMPLETE TABLE 4.3 (p110/text) Advantages and Disadvantages of Recombinant Protein Production in E.coli ADVANTAGES DISADVANTAGES</p><p>17. What is proteomics? Definition: ______</p><p>______</p>