<p> OIE Reference Laboratory Reports Activities in 2010</p><p>Name of disease (or topic) for Contagious bovine pleuropneumonia which you are a designated OIE Reference Laboratory:</p><p>Address of laboratory Istituto G. Caporale Teramo, Via Campo Boario, 64100 Teramo, Italy</p><p>Tel.: (+39-0861) 33.22.05</p><p>Fax: (+39-0861) 33.22.51</p><p> e-mail address: [email protected]</p><p> website: www.izs.it</p><p>Name of Head of Laboratory Vincenzo Caporale (Responsible Official):</p><p>Name of OIE Reference Expert: Attilio Pini</p><p>Name of writer of this report (if different from above):</p><p>Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 1 Contagious bovine pleuropneumonia</p><p>Part I: Summary of general activities related to the disease</p><p>1. Test(s) in use/or available for the specified disease/topic at your laboratory</p><p>Antigen for Complement fixation test, ELISA, PCR, Immunoblotting, Immunohistochemistry</p><p>Test For Specificity Total</p><p>CFT Antibody Group 29</p><p>ELISA Antibody Group 3</p><p>PCR-RFLP Antigen Specific 36</p><p>Real Time PCR to be validated Antigen Specific 0</p><p>Immunoblotting Antigen MmmSC 0</p><p>Immunohistochemistry Antigen Group 0</p><p>2. Production and distribution of diagnostic reagents </p><p>Amount supplied nationally Amount supplied to other Type of reagent (including for own use) countries</p><p>Control positive serum 40 ml 5 ml</p><p>Control negative serum 92 ml 2 ml</p><p>Antigen for CFT 12 ml 15 ml</p><p>Part II: Activities specifically related to the mandate of OIE Reference Laboratories</p><p>3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines</p><p>–</p><p>4. Preparation and supply of international reference standards for diagnostic tests or vaccines</p><p>Available: positive reference serum for CFT. Supplied to OIE Members: 5ml </p><p>5. Research and development of new procedures for diagnosis and control</p><p>–</p><p>6. Collection, analysis and dissemination of epizootiological data relevant to international disease control</p><p>–</p><p>2 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 Contagious bovine pleuropneumonia</p><p>7. Provision of consultant expertise to OIE or to OIE Members</p><p>Participation to the Workshop organised by the Department of Veterinary Services, Zambian Ministry of Livestock and Fisheries Development for providing advice in the drawing up of the programme aimed to control and eradicates CBPP. Lusaka, Zambia 26-30 May 2010.</p><p>8. Provision of scientific and technical training to personnel from other OIE Members</p><p>1. OIE Twinning Project between the Teramo Institute and the National Center for Animal and Plant Health (CENSA), Cuba. a. One Veterinarian and one biologist were trained in the Laboratories of G. Caporale Institute on anatomo-pathology of the disease, tissue sampling, microbiological culture, mycoplasma identification by PCR. (March-April 2010) b. A 3 days training course on “Mycoplasmas of Ruminants” was held on 12-14 October 2010 at CENSA, La Habana, Cuba 2. OIE Twinning Project between the Teramo Institute and the Botswana National Veterinary Laboratory (BNVL) Three specialists from the Institute went to the BNVL for final training on diagnostic procedures and production of diagnostic reagents. (27 April – 21 May 2010)</p><p>9. Provision of diagnostic testing facilities to other OIE Members</p><p>–</p><p>10. Organisation of international scientific meetings on behalf of OIE or other international bodies</p><p>Final Meeting on the occasion of the closure of the Twinning Project between the Institute and BNVL: Presentation of the BNVL as OIE Reference Laboratory candidate, Gaborone, Botswana. Co-organiser BNVL, 20 participants, including representatives of Veterinary Services from South Africa, Namibia, Angola and Zambia. Conclusions & recommendations provided with the final report of the Twinning Project.</p><p>11. Participation in international scientific collaborative studies</p><p>1. Collaborative research study in progress: Identification of novel immunogenic proteins of MmmSC for the development of a vaccine able to induce a cell mediated immune response. Objective: development of a recombinant vaccine based on MmmSC target proteins; outcome: recombinant vaccine. In collaboration with the Moredum Research Institute, Bacterial Disease Division, Pentlands Science Park, Scotland, UK, International, the Livestock Research Institute of Nairobi (Kenia), the Central Veterinary Laboratory Windhoek, Namibia and the Central Veterinary Laboratory, Balmoral, Zambia. 2. Assessment of the regulatory T cell immune response in the course of Mycoplasma mycoides subsp mycoides SC experimental infections. The project aimed to: a) Investigate the immune pathogenic mechanisms involved in MmmSC infections; b) Analyse, quantitatively and qualitatively, the distribution of bovine B and T lymphocyte components, antibody isotypes and cytokine profile in order to understand humoral and cell- mediated reactivity during MmmSC infections. The project was run in collaboration with the International Livestock Research Institute of Nairobi, Kenya, VLA of Weybridge (UK), the Central Veterinary Laboratory, Balmoral, Zambia and the Central Veterinary Laboratory of Windhoek, Namibia. The project started in march 2007 and was completed in march 2010. The project highlighted some new aspects of CBPP pathogenic mechanisms. </p><p>Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010 3 Contagious bovine pleuropneumonia</p><p>12. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) </p><p> Presentations at international conferences and meetings</p><p>1. Jorg Jores, Anja Sterner-Kock, Wolfram Haider, Flavio Sacchini, Flora Mbithi, Jan Naessens 2010. Role of Acquired Immunity in Protection of Cattle against Contagious Bovine Pleuropneumonia. Abstract book pag. 115, 18th Congress of the International Organization for Mycoplasmology, July 11-16, Chianciano Terme – Siena.</p><p>2. Flavio Sacchini, Enzo Langella, Mirella Luciani, Antonella Ciarelli, Romolo Salini, Attilio Pini, Jörg Jores & Jan Naessens 2010. Dynamics of TNF-α, IL-4, IFN-γ and IL-10 In MmmSC-Infected Cattle Depleted Or Non-Depleted For CD4+ T Lymphocytes. Abstract book pag. 29, 18th Congress of the International Organization for Mycoplasmology, July 11-16, Chianciano Terme – Siena.</p><p> Scientific publications in peer-reviewed journals</p><p>Anna Rita D’Angelo, Flavio Sacchini,Tiziana Di Febo,Vincenzo Langella, Andrea Di Provvido, Gabriella Di Francesco & Attilio Pini. 2010. Effects of immune serum on macrophage cell cultures infected with MmmSC: morphological scanning by electron microscopy. Veterinaria Italiana 46 (4), 389-396. </p><p>13. Inscription of diagnostic kits on the OIE Register</p><p> i) Did you participate in expert panels for the validation of candidate kits for inscription on the OIE Register? If yes, for which kits? –</p><p> ii) Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for which kits? –</p><p>______</p><p>4 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010</p>