<p>Electronic supplementary material</p><p>Table 1 PCR primers and conditions</p><p>Gene Sense Antisense Number of cycles Size of product (bp) Annealing temperature</p><p>(°C) Rat Insulin I CTACAATCATAGACCATC CAGTTGGTAGAGGGAGCAGAT 35 353 56 AGCA Rat Insulin 2 AGCCCTAAGTGACCAGCT GTGCCAAGGTCTGAAGGTCA 35 297 58</p><p>ACA Rat Pdx1 TGCTAATCCCCCTGCGTG CTCCTCCGGTTCTGCTGCGTATGC 40 348 59</p><p>CCTGTA Human PDX1 CAAGGACCCATGCGCGTT GAACTCCTTCTCCAGCTCTAGCA 40 453 62</p><p>CCAGCGA GCTG Rat Pksc1 TCAGGGAATGGGGGTCG CCAAATCGGCTGTTCACCATCAA 35 401 60</p><p>(PC1/3) TCAAG G Rat Slc2a2 (Glut2) TGGGTTCCTTCCAGTTCG AGGCGTCTGGTGTCGTATG 35 183 55</p><p>Rat Gck GTGGTGCTTTTGAGACCC TTCGATGAAGGTGATTTCGCA 35 107 55</p><p>GTT Rat Abcc8 (SUR1) TCACGCCTGTCTATCATC GCTTCATCCATGATGAAGATGC 35 265 58</p><p>CTACAG Rat Kcnj8 and 11 TACCACGTCATCGACTCC TGCGGTCCTCATCAAGCTGGC 35 268 58</p><p>(KIR6.1 and 6.2) AACAG Rat glucagon GACCGTTTACATCGTGGC GGTTCCTCTTGGTGTTCATCA 35 248 58</p><p>TG Rat somatostatin ACCCCAGACTCCGTCAGT CTCCAGCCTCATCTCGTCCT 35 173 58</p><p>TT Rat pancreatic AACAGAGGGCTCAATAC GAGACAGAAGGGAGGCTACA 35 302 56 polypeptide GAA</p><p>Rat amylase CAAAATGGTTCTCCCAAG CAAACACAAGGGCTCTGTCA 35 231 52 GA Rat albumin GCCCTACCCACAAAGCCT AGCCCCTTCATATCACAGAGCA 35 251 58</p><p>CAG Rat Pax4 GGGCACTACAGGAAGAC TTGGAAAACCAGACCCTCAC 40 274 60</p><p>CAG Rat Pax6 ATGCAGAACAGTCACAG TCGCTAGCCAGGTTGCGAAG 40 398 60</p><p>CGG Rat Nkx6-1 ACTTGGCAGGACCAGAG GGGCTTGTTGTAATCGTCGT 40 209 60</p><p>AGA Rat Neurod1 GAGGAGGAAGAGGAGGA GACGAGGTCTGGGCTTTTG 40 297 65</p><p>GGA Rat Mafa AGGCCTTCCGGGGCCAG TGGAGCTGGCACTTCTCGCT 40 408 60</p><p>AG Fig. 1 a Histomorphology of hepatocyte graft. Tissue sections (haematoxylin and eosin stained) of a SCID-beige mouse kidney with rat hepatocytes graft 60 days after transplantation. K, kidney parenchyma; continuous arrow, graft; dashed arrow, blood vessel within the graft. b Detection of insulin within the graft. Insulin was detected with an anti-insulin antibody (brown). The nuclei are stained with haematoxylin. G, graft; K, kidney parenchyma. Original magnifications: 100× (a), 400× (b); enlarged magnification of insulin-positive cells within graft (1000×) </p>