Table 1 PCR Primers and Conditions
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Table 1 PCR primers and conditions
Gene Sense Antisense Number of cycles Size of product (bp) Annealing temperature
(°C) Rat Insulin I CTACAATCATAGACCATC CAGTTGGTAGAGGGAGCAGAT 35 353 56 AGCA Rat Insulin 2 AGCCCTAAGTGACCAGCT GTGCCAAGGTCTGAAGGTCA 35 297 58
ACA Rat Pdx1 TGCTAATCCCCCTGCGTG CTCCTCCGGTTCTGCTGCGTATGC 40 348 59
CCTGTA Human PDX1 CAAGGACCCATGCGCGTT GAACTCCTTCTCCAGCTCTAGCA 40 453 62
CCAGCGA GCTG Rat Pksc1 TCAGGGAATGGGGGTCG CCAAATCGGCTGTTCACCATCAA 35 401 60
(PC1/3) TCAAG G Rat Slc2a2 (Glut2) TGGGTTCCTTCCAGTTCG AGGCGTCTGGTGTCGTATG 35 183 55
Rat Gck GTGGTGCTTTTGAGACCC TTCGATGAAGGTGATTTCGCA 35 107 55
GTT Rat Abcc8 (SUR1) TCACGCCTGTCTATCATC GCTTCATCCATGATGAAGATGC 35 265 58
CTACAG Rat Kcnj8 and 11 TACCACGTCATCGACTCC TGCGGTCCTCATCAAGCTGGC 35 268 58
(KIR6.1 and 6.2) AACAG Rat glucagon GACCGTTTACATCGTGGC GGTTCCTCTTGGTGTTCATCA 35 248 58
TG Rat somatostatin ACCCCAGACTCCGTCAGT CTCCAGCCTCATCTCGTCCT 35 173 58
TT Rat pancreatic AACAGAGGGCTCAATAC GAGACAGAAGGGAGGCTACA 35 302 56 polypeptide GAA
Rat amylase CAAAATGGTTCTCCCAAG CAAACACAAGGGCTCTGTCA 35 231 52 GA Rat albumin GCCCTACCCACAAAGCCT AGCCCCTTCATATCACAGAGCA 35 251 58
CAG Rat Pax4 GGGCACTACAGGAAGAC TTGGAAAACCAGACCCTCAC 40 274 60
CAG Rat Pax6 ATGCAGAACAGTCACAG TCGCTAGCCAGGTTGCGAAG 40 398 60
CGG Rat Nkx6-1 ACTTGGCAGGACCAGAG GGGCTTGTTGTAATCGTCGT 40 209 60
AGA Rat Neurod1 GAGGAGGAAGAGGAGGA GACGAGGTCTGGGCTTTTG 40 297 65
GGA Rat Mafa AGGCCTTCCGGGGCCAG TGGAGCTGGCACTTCTCGCT 40 408 60
AG Fig. 1 a Histomorphology of hepatocyte graft. Tissue sections (haematoxylin and eosin stained) of a SCID-beige mouse kidney with rat hepatocytes graft 60 days after transplantation. K, kidney parenchyma; continuous arrow, graft; dashed arrow, blood vessel within the graft. b Detection of insulin within the graft. Insulin was detected with an anti-insulin antibody (brown). The nuclei are stained with haematoxylin. G, graft; K, kidney parenchyma. Original magnifications: 100× (a), 400× (b); enlarged magnification of insulin-positive cells within graft (1000×)