![Supplemental Table 1. Primers and Restriction Enzymes Used to Make DNA Constructs](https://data.docslib.org/img/3a60ab92a6e30910dab9bd827208bcff-1.webp)
<p>Supplemental Table 1. Primers and restriction enzymes used to make DNA constructs.</p><p>Source of insert DNA Restriction DNA construct name Vector Primers or PCR template sites 2xGFP from 3xGFP vector (Kim pSAT6-2xGFP pSAT6-EGFP-N1 NcoI/EcoRI N/A et al. 2005) AK1cDNcF and pSAT6-ADK1-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI ADK1cR-Sma pSAT6-ADK1p:ADK1- ADK1pF-Age and ADK1 WT Arabidopsis genomic DNA pSAT6-ADK1-EGFP AgeI/NcoI EGFP lux lower SAHH1cDNcF and pSAT6-SAHH1-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI SAHH1cR-Sma SAHH1cF-Kpn and pSAT6-EGFP-SAHH1 WT Arabidopsis cDNA pSAT6-EGFP-C1 KpnI/XmaI SAHH1cR-Sma pSAT6- SAHH1pF-Age and WT Arabidopsis genomic DNA pSAT6-SAHH1-EGFP AgeI/NcoI SAHH1p:SAHH1-EGFP SAHH1Nc-R SAHH2for and pSAT6-SAHH2-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI SAHH2cR-Sma pSAT6- SAHH2pF-Age and WT Arabidopsis genomic DNA pSAT6-SAHH2-EGFP AgeI/NcoI SAHH2p:SAHH2-EGFP SAHH2Nc-R AK1cDNcF and pSAT6-ADK1-2xGFP WT Arabidopsis cDNA pSAT6-2xGFP NcoI AK1cDNcR SAHH1cDNcF and pSAT6-SAHH1-2xGFP WT Arabidopsis cDNA pSAT6-2xGFP NcoI SAHH1rev pSAT6-SV40NLS- NLS-DsRed2F and pSAT6-DsRed2-N1 pSAT6-DsRed2-N1 NcoI DsRed2 DsRed2R-Nco pSAT4-SV40NLS- pSAT6-SV40NLS-DsRed2 pSAT4-EGFP-N1 AgeI/NotI N/A DsRed2 pSAT6-SAHH1(Δ1-28)- SAHH1-D29F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI EGFP SAHH1cR-Sma pSAT6-SAHH1(Δ1-62)- SAHH1-D63F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI EGFP SAHH1cR-Sma pSAT6-SAHH1(Δ411- SAHH1cDNcF and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI 485)-EGFP SAHH1-D410R</p><p>1/4 pSAT6-SAHH1(Δ1-28, SAHH1-D29F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI Δ411-485)-EGFP SAHH1-D410R pSAT6-SAHH1(Δ1-62, SAHH1-D63F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI Δ411-485)-EGFP SAHH1-D410R pSAT6-SAHH(1-149)- SAHH1cDNcF and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/PstI EGFP SAHH1-D149R pSAT6-SAHH1(Δ150- pSAT6-SAHH(1-149)- SAHH1-D191F and WT Arabidopsis cDNA PstI/XmaI 190)-EGFP EGFP SAHH1cR-Sma SAHH1-149F and pSAT6-IR-2xGFP WT Arabidopsis cDNA pSAT6-2xGFP NcoI SAHH1-190R pJET1.2 (Fermentas, ySAHH-F1 and ySAHH- pJET-ySAHH Yeast genomic DNA N/A Canada) R1 ySAHH-F Xho2 and pSAT6-EGFP-ySAHH pJET-ySAHH pSAT6-EGFP-C1 XhoI ySAHH-R Xho CMT-F Nco and CMT-R pSAT6-CMT-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/BamHI Bam CMT-F Hin and CMT-R pSAT6-EGFP-CMT WT Arabidopsis cDNA pSAT6-EGFP-C1 HindIII/BamHI Bam pSAT6-nCMT-EGFP pSAT6-CMT-EGFP pSAT6-EGFP-N1 NcoI/PstI N/A pSAT6-cCMT-EGFP pSAT6-CMT-EGFP pSAT6-EGFP-N1 PstI/BamHI N/A pSAT4-ADK1-YN pSAT6-ADK1-EGFP pSAT4-YN-N1 NcoI/XmaI N/A pSAT6-YC-N1 pSAT1-YC-N1 pSAT6-EGFP-N1 AgeI/NotI N/A pSAT6-SAHH1-YC pSAT6-SAHH1-EGFP pSAT6-YC-N1 NcoI/XmaI N/A pSAT4-SAHH1-YN pSAT6-SAHH1-EGFP pSAT4-YN-N1 NcoI/BamHI N/A pSAT6-SAHH1(ΔIR)-YC pSAT6-SAHH(Δ150-190)-EGFP pSAT6-YC-N1 NcoI/XmaI N/A pSAT4-CMT-YN pSAT6-CMT-EGFP pSAT4-YN-N1 NcoI/BamHI N/A pSAT6-CMT-YC pSAT6-CMT-EGFP pSAT6-YC-N1 NcoI/BamHI N/A Impa-F Eco and Impa-R pGST-Impα WT Arabidopsis cDNA pGEX4T-1 EcoRI/XhoI Xho</p><p>2/4 CMT-F Bam and CMT-R pGST-CMT WT Arabidopsis cDNA pGEX4T-1 BamHI Bam</p><p>Primer name Primer sequence Restriction sites AK1cDNcF 5-CATccatggCTTCCTCTGATTTC-3 NcoI ADK1cR-Sma 5-ATcccgggGTTGAAGTCTGGTTTCTC-3 XmaI ADK1pF-Age 5- TTaccggtCACCTAAATTGTTTGCCTATTCT-3 AgeI ADK1 lux lower 5-GTACGccatggATGATGATGTTAGAAGG-3 NcoI AK1cDNcR 5-GAccatggTGAAGTCTGGTTTCTC-3 NcoI SAHH1cDNcF 5-TCAAccatggCGTTGCTCGTC-3 NcoI SAHH1cR-Sma 5-TTTcccgggGTACCTGTAGTGAGGAGGCTT-3 XmaI SAHH1cF-Kpn 5-TAAggtaccATGGCGTTGCTCGTCGAGAAG-3 KpnI SAHH1pF-Age 5-CATaccggtGATCCACCGACGTTCCTAC-3 AgeI SAHH1Nc-R 5-GCAACGccatggTTGAGCTAGATC-3 NcoI SAHH1rev 5-CTCCATGGACCTGTAGTGAGGAGGCTT-3 NcoI SAHH2for 5-CAAccatggCTTTGCTTGTAGAGAAAACC-3 NcoI SAHH2cR-Sma 5-GTTcccgggGTACCTGTAGTGAACAGGCTTG-3 XmaI SAHH2pF-Age 5-CAaccggtCCTTAAATTGCAGGAAC-3 AgeI SAHH2Nc-R 5-GCAAAGccatggTTGAATAGTTTC-3 NcoI 5-ATccatggTGCCAAAAAAGAAGAGAAAGGTAGAAGACCCCGTG NLS-DsRed2F NcoI ATGGCCTCCTCCGAGAACG-3 DsRed2R-Nco 5-AGCccatggTCTTCTTCTGCATC-3 NcoI SAHH1-D29F 5-GATccatggATCTCGAGCTCGCCGAAGTTG-3 NcoI</p><p>3/4 SAHH1-D63F 5-CATccatggATATGACCATCCAAACCGCCGTAC-3 NcoI SAHH1-D410R 5-GTTcccgggAGACATCACGAAACTTGGGTG-3 XmaI SAHH1-D149R 5-CAActgcagCTCATGAATCAAAAGAGTAG-3 PstI SAHH1-D191F 5-GAGctgcagAAGTACCACAAGATGAAGGAG-3 PstI SAHH1-149F 5-AAccatggAGGGTGTTAAAGCTGAG-3 NcoI SAHH1-190R 5-ATccatggTCTTAGGATCAACTTGAAG-3 NcoI ySAHH-F1 5-GAACAATTCCCAATGTCTGCTC-3 N/A ySAHH-R1 5-ATATCTGTAGTGGTCGGCCTTG-3 N/A ySAHH-F Xho2 5-AActcgagACATGTCTGCTCCAGCTC-3 XhoI ySAHH-R Xho 5-AActcgagTCAATATCTGTAGTGGTCGG-3 XhoI CMT-F Nco 5-CTTccatggTTATGAAACGAGGATTCTCCG -3 NcoI CMT-R Bam 5-CTAggatccAGCTATCGATATACAAAACATC-3 BamHI CMT-F Hin 5-CTTaagcttGTATGAAACGAGGATTCTCCG-3 HindIII CMT-F Bam 5-CTTggatccATGAAACGAGGATTCTC-3 BamHI Impa-F Eco 5-AAgaattcATGTCACTGAGACCCAACGC-3 EcoRI Impa-R Xho 5-AActcgagTCAGCTGAAGTTGAATCCTC-3 XhoI</p><p>4/4</p>
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