Supplemental Table 1. Primers and Restriction Enzymes Used to Make DNA Constructs

Supplemental Table 1. Primers and restriction enzymes used to make DNA constructs.

Source of insert DNA Restriction DNA construct name Vector Primers or PCR template sites 2xGFP from 3xGFP vector (Kim pSAT6-2xGFP pSAT6-EGFP-N1 NcoI/EcoRI N/A et al. 2005) AK1cDNcF and pSAT6-ADK1-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI ADK1cR-Sma pSAT6-ADK1p:ADK1- ADK1pF-Age and ADK1 WT Arabidopsis genomic DNA pSAT6-ADK1-EGFP AgeI/NcoI EGFP lux lower SAHH1cDNcF and pSAT6-SAHH1-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI SAHH1cR-Sma SAHH1cF-Kpn and pSAT6-EGFP-SAHH1 WT Arabidopsis cDNA pSAT6-EGFP-C1 KpnI/XmaI SAHH1cR-Sma pSAT6- SAHH1pF-Age and WT Arabidopsis genomic DNA pSAT6-SAHH1-EGFP AgeI/NcoI SAHH1p:SAHH1-EGFP SAHH1Nc-R SAHH2for and pSAT6-SAHH2-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI SAHH2cR-Sma pSAT6- SAHH2pF-Age and WT Arabidopsis genomic DNA pSAT6-SAHH2-EGFP AgeI/NcoI SAHH2p:SAHH2-EGFP SAHH2Nc-R AK1cDNcF and pSAT6-ADK1-2xGFP WT Arabidopsis cDNA pSAT6-2xGFP NcoI AK1cDNcR SAHH1cDNcF and pSAT6-SAHH1-2xGFP WT Arabidopsis cDNA pSAT6-2xGFP NcoI SAHH1rev pSAT6-SV40NLS- NLS-DsRed2F and pSAT6-DsRed2-N1 pSAT6-DsRed2-N1 NcoI DsRed2 DsRed2R-Nco pSAT4-SV40NLS- pSAT6-SV40NLS-DsRed2 pSAT4-EGFP-N1 AgeI/NotI N/A DsRed2 pSAT6-SAHH1(Δ1-28)- SAHH1-D29F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI EGFP SAHH1cR-Sma pSAT6-SAHH1(Δ1-62)- SAHH1-D63F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI EGFP SAHH1cR-Sma pSAT6-SAHH1(Δ411- SAHH1cDNcF and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI 485)-EGFP SAHH1-D410R

1/4 pSAT6-SAHH1(Δ1-28, SAHH1-D29F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI Δ411-485)-EGFP SAHH1-D410R pSAT6-SAHH1(Δ1-62, SAHH1-D63F and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/XmaI Δ411-485)-EGFP SAHH1-D410R pSAT6-SAHH(1-149)- SAHH1cDNcF and WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/PstI EGFP SAHH1-D149R pSAT6-SAHH1(Δ150- pSAT6-SAHH(1-149)- SAHH1-D191F and WT Arabidopsis cDNA PstI/XmaI 190)-EGFP EGFP SAHH1cR-Sma SAHH1-149F and pSAT6-IR-2xGFP WT Arabidopsis cDNA pSAT6-2xGFP NcoI SAHH1-190R pJET1.2 (Fermentas, ySAHH-F1 and ySAHH- pJET-ySAHH Yeast genomic DNA N/A Canada) R1 ySAHH-F Xho2 and pSAT6-EGFP-ySAHH pJET-ySAHH pSAT6-EGFP-C1 XhoI ySAHH-R Xho CMT-F Nco and CMT-R pSAT6-CMT-EGFP WT Arabidopsis cDNA pSAT6-EGFP-N1 NcoI/BamHI Bam CMT-F Hin and CMT-R pSAT6-EGFP-CMT WT Arabidopsis cDNA pSAT6-EGFP-C1 HindIII/BamHI Bam pSAT6-nCMT-EGFP pSAT6-CMT-EGFP pSAT6-EGFP-N1 NcoI/PstI N/A pSAT6-cCMT-EGFP pSAT6-CMT-EGFP pSAT6-EGFP-N1 PstI/BamHI N/A pSAT4-ADK1-YN pSAT6-ADK1-EGFP pSAT4-YN-N1 NcoI/XmaI N/A pSAT6-YC-N1 pSAT1-YC-N1 pSAT6-EGFP-N1 AgeI/NotI N/A pSAT6-SAHH1-YC pSAT6-SAHH1-EGFP pSAT6-YC-N1 NcoI/XmaI N/A pSAT4-SAHH1-YN pSAT6-SAHH1-EGFP pSAT4-YN-N1 NcoI/BamHI N/A pSAT6-SAHH1(ΔIR)-YC pSAT6-SAHH(Δ150-190)-EGFP pSAT6-YC-N1 NcoI/XmaI N/A pSAT4-CMT-YN pSAT6-CMT-EGFP pSAT4-YN-N1 NcoI/BamHI N/A pSAT6-CMT-YC pSAT6-CMT-EGFP pSAT6-YC-N1 NcoI/BamHI N/A Impa-F Eco and Impa-R pGST-Impα WT Arabidopsis cDNA pGEX4T-1 EcoRI/XhoI Xho

2/4 CMT-F Bam and CMT-R pGST-CMT WT Arabidopsis cDNA pGEX4T-1 BamHI Bam

Primer name Primer sequence Restriction sites AK1cDNcF 5-CATccatggCTTCCTCTGATTTC-3 NcoI ADK1cR-Sma 5-ATcccgggGTTGAAGTCTGGTTTCTC-3 XmaI ADK1pF-Age 5- TTaccggtCACCTAAATTGTTTGCCTATTCT-3 AgeI ADK1 lux lower 5-GTACGccatggATGATGATGTTAGAAGG-3 NcoI AK1cDNcR 5-GAccatggTGAAGTCTGGTTTCTC-3 NcoI SAHH1cDNcF 5-TCAAccatggCGTTGCTCGTC-3 NcoI SAHH1cR-Sma 5-TTTcccgggGTACCTGTAGTGAGGAGGCTT-3 XmaI SAHH1cF-Kpn 5-TAAggtaccATGGCGTTGCTCGTCGAGAAG-3 KpnI SAHH1pF-Age 5-CATaccggtGATCCACCGACGTTCCTAC-3 AgeI SAHH1Nc-R 5-GCAACGccatggTTGAGCTAGATC-3 NcoI SAHH1rev 5-CTCCATGGACCTGTAGTGAGGAGGCTT-3 NcoI SAHH2for 5-CAAccatggCTTTGCTTGTAGAGAAAACC-3 NcoI SAHH2cR-Sma 5-GTTcccgggGTACCTGTAGTGAACAGGCTTG-3 XmaI SAHH2pF-Age 5-CAaccggtCCTTAAATTGCAGGAAC-3 AgeI SAHH2Nc-R 5-GCAAAGccatggTTGAATAGTTTC-3 NcoI 5-ATccatggTGCCAAAAAAGAAGAGAAAGGTAGAAGACCCCGTG NLS-DsRed2F NcoI ATGGCCTCCTCCGAGAACG-3 DsRed2R-Nco 5-AGCccatggTCTTCTTCTGCATC-3 NcoI SAHH1-D29F 5-GATccatggATCTCGAGCTCGCCGAAGTTG-3 NcoI

3/4 SAHH1-D63F 5-CATccatggATATGACCATCCAAACCGCCGTAC-3 NcoI SAHH1-D410R 5-GTTcccgggAGACATCACGAAACTTGGGTG-3 XmaI SAHH1-D149R 5-CAActgcagCTCATGAATCAAAAGAGTAG-3 PstI SAHH1-D191F 5-GAGctgcagAAGTACCACAAGATGAAGGAG-3 PstI SAHH1-149F 5-AAccatggAGGGTGTTAAAGCTGAG-3 NcoI SAHH1-190R 5-ATccatggTCTTAGGATCAACTTGAAG-3 NcoI ySAHH-F1 5-GAACAATTCCCAATGTCTGCTC-3 N/A ySAHH-R1 5-ATATCTGTAGTGGTCGGCCTTG-3 N/A ySAHH-F Xho2 5-AActcgagACATGTCTGCTCCAGCTC-3 XhoI ySAHH-R Xho 5-AActcgagTCAATATCTGTAGTGGTCGG-3 XhoI CMT-F Nco 5-CTTccatggTTATGAAACGAGGATTCTCCG -3 NcoI CMT-R Bam 5-CTAggatccAGCTATCGATATACAAAACATC-3 BamHI CMT-F Hin 5-CTTaagcttGTATGAAACGAGGATTCTCCG-3 HindIII CMT-F Bam 5-CTTggatccATGAAACGAGGATTCTC-3 BamHI Impa-F Eco 5-AAgaattcATGTCACTGAGACCCAACGC-3 EcoRI Impa-R Xho 5-AActcgagTCAGCTGAAGTTGAATCCTC-3 XhoI