<p> DOC/LP/01/28.02.02 LESSON PLAN LP- BT 2353 LP Rev. No: 00</p><p>SUB CODE & NAME : BT 2353 / BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: I BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6</p><p>I Unit: ANALYSIS OF STR Stirred tank reactor - non-ideality, RTD and stability analysis, tanks in series and dispersion models – application to design of continuous sterilizer.</p><p>Session Topics to be covered Time Ref Teaching Page No No (min) Aids (TB/REF) 1. Introduction to bioprocess engineering and the 50 TB2 OHP 533-534 various types of reactors used. 2. Characteristics of Stirred tank reactor, its 50 RB1 BB, 146 configurations 3. Non-ideal behaviour in a reactor , its types and factors 50 TB2 OHP 551-553 causing them , possibilities to eliminate non- ideality 4. Residence time distribution and its role in mixing 50 TB2 BB 553-560</p><p>5. Stability analysis of a stirred tank reactor 50 TB2 BB 547-551</p><p>6. Tanks in series model –mixing characteristics 50 TB2 BB 563-569</p><p>7. Dispersion model –mixing characteristics 50 TB2 BB 569-572</p><p>8. Application of Tanks in series model & Dispersion 50 TB2 BB 586-595 model to design of continuous sterilizers 9. Problems related to tanks in series model and 50 -- BB -- dispersion model</p><p>DOC/LP/01/28.02.02 LESSON PLAN LP- BT 2353 LP Rev. No: 00</p><p>SUB CODE & NAME : BT2353 / BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: II BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6</p><p>II Unit: ANALYSIS OF OTHER CONFIGURATIONS Packed bed reactor, airlift reactor, fluidized bed reactor bubble column reactors – non-ideality, RTD and stability analysis.</p><p>Session Topics to be covered Time Ref Teaching Page No No (min) Aids (TB/REF) 10 Packed bed reactor – configurations, reasons for non- 50 TB2 OHP 609-610 . ideality,Advantages, disadvantages, its applications</p><p>11 RTD and stability analysis for a Packed bed reactor 50 TB2 BB 553-547 .</p><p>12 Airlift reactor – configurations, reasons for non-ideality, 50 TB2 OHP 641 . Advantages, disadvantages, its applications</p><p>13 RTD and stability analysis for a Airlift reactor 50 TB2 BB 553-547 .</p><p>14 Fluidized bed reactor –configurations, reasons for non- 50 TB2 OHP 614-617 . ideality,Advantages, disadvantages, its applications</p><p>15 RTD and stability analysis for a Fluidized bed reactor 50 TB2 BB 553-547 .</p><p>16 Bubble column reactor– configurations, reasons for non- 50 TB2 OHP 610-614 . ideality, Advantages, disadvantages, its applications</p><p>17 RTD and stability analysis for a Bubble column reactor 50 TB2 BB 553-547 .</p><p>18 Review 50 ------. LESSON PLAN LP- BT 2353 LP Rev. No: 00</p><p>SUB CODE & NAME : BT2353 / BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: III BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6</p><p>DOC/LP/01/28.02.02</p><p>III Unit: BIOREACTOR SCALE – UP Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors - microbial oxygen demands; methods for the determination of mass transfer coefficients; mass transfer correlations. Scale up criteria for bioreactors based on oxygen transfer, power consumption and impeller tip speed.</p><p>Session Time Ref Teaching Page No No Topics to be covered (min) Aids (TB/REF) 19. Regime analysis of bioreactor processes 50 RB 1 BB 240-247</p><p>20. Gas liquid mass transfer , oxygen mass transfer 50 RB 1 OHP 241-242 in bioreactors 21. Oxygen demand, oxygen uptake ,dissolved 50 RB 1 BB 261-262 oxygen concentration, 22. methods for the determination of mass transfer 50 RB 1 BB 264-265 coefficient -dynamic gas out-gas in method 23. Sulphite oxidation method for the determination 50 RB 1 BB 263 of mass transfer coefficient 24. Other methods for the determination of mass 50 RB 1 BB 265 transfer coefficient 25. mass transfer correlations 50 RB 1 BB 248</p><p>26. Scale up criteria for bioreactor- oxygen transfer, 50 RB 1 BB 274</p><p>27. Scale up criteria for bioreactor- power 50 RB 1 BB 272 consumption 28. Scale up criteria for bioreactor- impeller tip 50 RB 1 BB 274 speed 29. Scale up criteria for bioreactor- other 50 RB 1 BB 273 considerations</p><p>DOC/LP/01/28.02.02 LESSON PLAN LP- BT 2353 LP Rev. No: 00</p><p>SUB CODE & NAME : BT2353/ BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: IV BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6</p><p>IV Unit: MODELLING AND SIMULATION OF BIOPROCESSES Study of structured models for analysis of various bioprocesses – compartmental models, models of cellular energetics and metabolism, single cell models, plasmid replication and plasmid stability model. Dynamic simulation of batch, fed batch, steady and transient culture metabolism.</p><p>Session Time Ref Teaching Page No No Topics to be covered (min Aids (TB/REF) ) 30. Introduction to modeling and simulation 50 RB1 BB 174</p><p>31. compartmental models 50 RB1 & OHP 176, RB 3 227 32. models of cellular energetics 50 RB 3 BB 230-244</p><p>33. models of cellular metabolism 50 RB 3 OHP 230-244</p><p>34. single cell models 50 RB3 OHP 244-246</p><p>35. plasmid replication 50 RB3 BB 251-257</p><p>36. plasmid stability model 50 RB3 BB 251-257</p><p>37. Dynamic simulation of batch culture metabolism 50 RB3 BB 277-280</p><p>38. Dynamic simulation of fed batch culture 50 RB3 BB 305-308 metabolism 39. Dynamic simulation of steady state culture 50 RB3 BB 280-296 metabolism LP- BT 2353 40. Dynamic simulation of transient cultureLESSON PLAN50 RB3 BB 309-322 metabolism LP Rev. No: 00 SUB CODE & NAME : BT2353/ BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: V BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6</p><p>DOC/LP/01/28.02.02</p><p>V Unit: BIOREACTOR CONSIDERATION IN ENZYME SYSTEMS Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions; formulation of dimensionless groups and calculation of effectiveness factors. Design of immobilized enzyme reactors – packed bed, fluidized bed and membrane reactors.</p><p>Session Topics to be covered Time Ref Teaching Page No No (min) Aids (TB/REF) 41. Kinetics of immobilized enzyme 50 RB1& OHP 202, reactions RB 3 116-137 42. Film diffusion effects 50 RB 3 BB 216</p><p>43. Pore diffusion effects 50 RB 3 BB 208</p><p>44. Formulation of dimensionless 50 RB 3 BB 128 groups 45. Calculation of effectiveness factors 50 RB 3 BB 122</p><p>46. Design of immobilized enzyme 50 TB2 OHP 609 reactors – packed bed reactor 47. Design of immobilized enzyme 50 TB2 OHP 614 reactors – fluidized bed reactor 48. Design of immobilized enzyme 50 TB2 OHP 610 reactors – membrane reactors Course Delivery Plan</p><p>Weeks 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Units I II III IV V</p><p>TEXT BOOKS 1. Anton Moser, “Bioprocess Technology”, Kinetics and Reactors”, Springer Verlag. 2. James E. Bailey & David F. Ollis, “Biochemical Engineering Fundamentals”, McGraw-Hill.</p><p>REFERENCES 1. James M. Lee, “Biochemical Engineering”, PHI, USA. 2. Atkinson, “Handbook of Bioreactors”, 3. Harvey W. Blanch, Douglas S. Clark, “Biochemical Engineering”, Marcel Decker Inc.</p><p>Prepared by Approved by Signature</p><p>Name V.Sumitha Prof. M. Sivanandham Designation Asst.Professor HOD of Biotechnology</p><p>Date 16-12-11 16-12-11 </p>