II Unit: ANALYSIS of OTHER CONFIGURATIONS

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SUB CODE & NAME : BT 2353 / BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: I BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6

I Unit: ANALYSIS OF STR Stirred tank reactor - non-ideality, RTD and stability analysis, tanks in series and dispersion models – application to design of continuous sterilizer.

Session Topics to be covered Time Ref Teaching Page No No (min) Aids (TB/REF) 1. Introduction to bioprocess engineering and the 50 TB2 OHP 533-534 various types of reactors used. 2. Characteristics of Stirred tank reactor, its 50 RB1 BB, 146 configurations 3. Non-ideal behaviour in a reactor , its types and factors 50 TB2 OHP 551-553 causing them , possibilities to eliminate non- ideality 4. Residence time distribution and its role in mixing 50 TB2 BB 553-560

5. Stability analysis of a stirred tank reactor 50 TB2 BB 547-551

6. Tanks in series model –mixing characteristics 50 TB2 BB 563-569

7. Dispersion model –mixing characteristics 50 TB2 BB 569-572

8. Application of Tanks in series model & Dispersion 50 TB2 BB 586-595 model to design of continuous sterilizers 9. Problems related to tanks in series model and 50 -- BB -- dispersion model

SUB CODE & NAME : BT2353 / BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: II BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6

II Unit: ANALYSIS OF OTHER CONFIGURATIONS Packed bed reactor, airlift reactor, fluidized bed reactor bubble column reactors – non-ideality, RTD and stability analysis.

Session Topics to be covered Time Ref Teaching Page No No (min) Aids (TB/REF) 10 Packed bed reactor – configurations, reasons for non- 50 TB2 OHP 609-610 . ideality,Advantages, disadvantages, its applications

11 RTD and stability analysis for a Packed bed reactor 50 TB2 BB 553-547 .

12 Airlift reactor – configurations, reasons for non-ideality, 50 TB2 OHP 641 . Advantages, disadvantages, its applications

13 RTD and stability analysis for a Airlift reactor 50 TB2 BB 553-547 .

14 Fluidized bed reactor –configurations, reasons for non- 50 TB2 OHP 614-617 . ideality,Advantages, disadvantages, its applications

15 RTD and stability analysis for a Fluidized bed reactor 50 TB2 BB 553-547 .

16 Bubble column reactor– configurations, reasons for non- 50 TB2 OHP 610-614 . ideality, Advantages, disadvantages, its applications

17 RTD and stability analysis for a Bubble column reactor 50 TB2 BB 553-547 .

18 Review 50 ------. LESSON PLAN LP- BT 2353 LP Rev. No: 00

SUB CODE & NAME : BT2353 / BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: III BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6

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III Unit: BIOREACTOR SCALE – UP Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors - microbial oxygen demands; methods for the determination of mass transfer coefficients; mass transfer correlations. Scale up criteria for bioreactors based on oxygen transfer, power consumption and impeller tip speed.

Session Time Ref Teaching Page No No Topics to be covered (min) Aids (TB/REF) 19. Regime analysis of bioreactor processes 50 RB 1 BB 240-247

20. Gas liquid mass transfer , oxygen mass transfer 50 RB 1 OHP 241-242 in bioreactors 21. Oxygen demand, oxygen uptake ,dissolved 50 RB 1 BB 261-262 oxygen concentration, 22. methods for the determination of mass transfer 50 RB 1 BB 264-265 coefficient -dynamic gas out-gas in method 23. Sulphite oxidation method for the determination 50 RB 1 BB 263 of mass transfer coefficient 24. Other methods for the determination of mass 50 RB 1 BB 265 transfer coefficient 25. mass transfer correlations 50 RB 1 BB 248

26. Scale up criteria for bioreactor- oxygen transfer, 50 RB 1 BB 274

27. Scale up criteria for bioreactor- power 50 RB 1 BB 272 consumption 28. Scale up criteria for bioreactor- impeller tip 50 RB 1 BB 274 speed 29. Scale up criteria for bioreactor- other 50 RB 1 BB 273 considerations

SUB CODE & NAME : BT2353/ BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: IV BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6

IV Unit: MODELLING AND SIMULATION OF BIOPROCESSES Study of structured models for analysis of various bioprocesses – compartmental models, models of cellular energetics and metabolism, single cell models, plasmid replication and plasmid stability model. Dynamic simulation of batch, fed batch, steady and transient culture metabolism.

Session Time Ref Teaching Page No No Topics to be covered (min Aids (TB/REF) ) 30. Introduction to modeling and simulation 50 RB1 BB 174

31. compartmental models 50 RB1 & OHP 176, RB 3 227 32. models of cellular energetics 50 RB 3 BB 230-244

33. models of cellular metabolism 50 RB 3 OHP 230-244

34. single cell models 50 RB3 OHP 244-246

35. plasmid replication 50 RB3 BB 251-257

36. plasmid stability model 50 RB3 BB 251-257

37. Dynamic simulation of batch culture metabolism 50 RB3 BB 277-280

38. Dynamic simulation of fed batch culture 50 RB3 BB 305-308 metabolism 39. Dynamic simulation of steady state culture 50 RB3 BB 280-296 metabolism LP- BT 2353 40. Dynamic simulation of transient cultureLESSON PLAN50 RB3 BB 309-322 metabolism LP Rev. No: 00 SUB CODE & NAME : BT2353/ BIOPROCESS ENGINEERING Date: 16-12-11 UNIT: V BRANCH: BIOTECHNOLOGY SEMESTER : VI Page 1 of 6

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V Unit: BIOREACTOR CONSIDERATION IN ENZYME SYSTEMS Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions; formulation of dimensionless groups and calculation of effectiveness factors. Design of immobilized enzyme reactors – packed bed, fluidized bed and membrane reactors.

Session Topics to be covered Time Ref Teaching Page No No (min) Aids (TB/REF) 41. Kinetics of immobilized enzyme 50 RB1& OHP 202, reactions RB 3 116-137 42. Film diffusion effects 50 RB 3 BB 216

43. Pore diffusion effects 50 RB 3 BB 208

44. Formulation of dimensionless 50 RB 3 BB 128 groups 45. Calculation of effectiveness factors 50 RB 3 BB 122

46. Design of immobilized enzyme 50 TB2 OHP 609 reactors – packed bed reactor 47. Design of immobilized enzyme 50 TB2 OHP 614 reactors – fluidized bed reactor 48. Design of immobilized enzyme 50 TB2 OHP 610 reactors – membrane reactors Course Delivery Plan

Weeks 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Units I II III IV V

TEXT BOOKS 1. Anton Moser, “Bioprocess Technology”, Kinetics and Reactors”, Springer Verlag. 2. James E. Bailey & David F. Ollis, “Biochemical Engineering Fundamentals”, McGraw-Hill.

REFERENCES 1. James M. Lee, “Biochemical Engineering”, PHI, USA. 2. Atkinson, “Handbook of Bioreactors”, 3. Harvey W. Blanch, Douglas S. Clark, “Biochemical Engineering”, Marcel Decker Inc.

Prepared by Approved by Signature

Name V.Sumitha Prof. M. Sivanandham Designation Asst.Professor HOD of Biotechnology

Date 16-12-11 16-12-11