<p>Supplementary Data</p><p>Title Diversity and bioprospective potential (cold-active enzymes) of cultivable marine bacteria from the subarctic glacial fjord, Kongsfjorden</p><p>Authors Sathish Prasad, Poorna Manasa, Sailaja Buddhi, Preethi Tirunagiri, Zareena Begum, Sivaraman Rajana, Sisinthy Shivaji*</p><p>Institution Centre for Cellular and Molecular Biology Uppal Road, Hyderabad, INDIA-500 007</p><p> aNational Centre for Antarctic and Ocean Research, Headland Sada, Vasco-da-Gama, Goa INDIA-403 804</p><p>Correspondence *Dr. S. Shivaji Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500007, INDIA Telephone:00-91-40-27192504 Fax: 00-91-40-27160311 Email: [email protected] Table S1. Kongsfjorden water and sediment sample details S.No Sa GPS Co-ordinates TVC±SD (n=3) Number of Temperature Salinity P F(µg/l) T(FTU) Density mpl l-1 water or Morphotypes (ºC) (ppt) r e g-1 sediment isolated e / s Sit s e u Co r de e ( p a ) 1 KJ 78°59'177''N/11°32'5 8.2±0.13x105 17 2.3 33.6 0 0.03 1.4 26.9 F1 96''E . 9 2 KJ 79º1’31”N/11º47’18” 3.5±0.20x105 18 3.0 33.5 1 0.03 0.7 26.7 F2 E . 1 3 KJ 78°55'242''N/12°5'74 1.9±0.39x106 37 2.7 32.6 0 0.1 1.1 26.0 F3 8''E . 8 4 KJ 78°57'428''N/12°09'8 2.9±1.08x106 19 2.3 31.2 1 0.1 1.3 24.9 F4 94''E . 4 5 KJ 78°53'667''N/12°29'4 6.1±0.17x105 15 2.6 32.5 0 0.1 2.2 25.9 F5 34''E . 7 6 KJ 78°53'504''N/12°22'5 5.2±0.04x106 16 2.3 31.5 0 0.2 5.2 25.1 F6 49''E . 5 7 KJ 78°57'42''N/ 1.7±0.61x105 8 3.9 33.0 0 1.2 12.3 26.2 F7 11°54'19''E . 3 8 KJ 78°57'90''N/11°57'09 2.2±1.19x105 22 4.3 28.3 0 0.03 0.6 22.5 F8 ''E . 5 9 KJ 78°58'39''N/12°00'00 1.3±0.12x106 18 4.3 24.3 0 0.04 1.6 19.3 F9 ''E . 1 10 KJ 78°56'94''N/12°01'94 2.1±0.76x105 15 4.0 32.5 1 0.3 1.5 25.8 F1 ''E . 0 1 11 KJ 78°56'29''N/12°09'67 2.2±0.37x105 26 4.3 32.4 0 0.2 1.6 25.7 F1 ''E . 1 4 12 KJ 78°56'77''N/12°11'61 7.2±0.29x105 22 4.5 22.4 0 0.2 1.5 17.8 F1 ''E . 2 6 13 KJ 78°57'10''N/12°13'55 3.4±0.41x106 17 4.2 32.3 0 0.6 4.7 25.6 F1 ''E . 3 9 14 KJ 78°58'23''N/12°18'39 1.4±0.25x106 22 3.7 31.1 0 0.2 3.9 24.7 F1 ''E . 4 3 15 KJ 78°58'39''N/12°25'16 3.5±0.33x105 19 3.3 31.0 1 0.2 1.6 24.6 F1 ''E . 5 1 16 FS 78°55'242''N/12°5'74 3.1±0.61×102 2 0.9 34.0 2 0.1 0.6 27.4 3 8''E 1 . 6 17 FS 78°57'428''N/12°09'8 4.5±0.64×104 4 0.3 34.1 2 0.2 0.9 27.5 4 94''E 0 . 7 18 FS 78°53'667''N/12°29'4 4.8±0.32×103 4 0.5 34.1 2 0.1 1.8 27.4 5 34''E 1 . 5 19 FS 78°53'504''N/12°22'5 3.8±0.58×103 2 0.8 33.9 2 0.1 2.6 27.3 6 49''E 0 . 8 20 FS 78°57'42''N/ 8.2±0.54×103 3 -1.1 34.4 5 0.1 0.5 27.9 7 11°54'19''E 3 . 7 21 FS 78°57'90''N/11°57'09 5.2±0.62×103 3 -1.3 34.3 5 0.3 0.4 27.9 8 ''E 5 . 2 22 FS 78°56'94''N/12°01'94 5.9±0.2×103 4 0.2 34.1 2 0.3 0.6 27.5 10 ''E 2 . 4 23 FS 78°56'29''N/12°09'67 1.1± 0.21×104 3 -0.1 34.2 2 0.4 0.6 27.6 11 ''E 1 . 2 24 FS 78°56'77''N/12°11'61 6.1±0.88×102 4 -0.4 34.2 2 0.1 0.8 27.6 12 ''E 2 . 9 25 FS 78°57'10''N/12°13'55 1.4± 0.33 ×104 6 -0.2 34.1 2 0.1 7.6 27.5 13 ''E 3 . 2 26 FS 78°58'23''N/12°18'39 1.9±0.33 ×104 5 -0.3 34.2 2 0.1 0.8 27.6 14 ''E 1 . 8 27 FS 78°58'39''N/12°25'16 1.1± 0.25 ×103 1 -0.5 34.1 2 0.1 2.1 27.5 15 ''E 3 . 2</p><p>TVC= Total Viable Counts ppt=parts per thousand (g/l), F= Fluorescence, T= Turbidity, FTU= Formazin Turbidity Units, NA=Not Available Table S2. Phylogenetic affiliation of the 291 bacterial isolates from Kongsfjorden water based on 16S rRNA gene sequence similarity </p><p>S.No Strain number (Accession No.) [16S rRNA gene sequence similarity (%)] Closest Type Strain Phylum Actinobacteria Class Actinobacteria 1. KJF1-15, KJF5-12, KJF10-8, [99.4], KJF1-8, KJF8-7 (JQ800123) [99.6], KJF8-25 [99.2], Salinibacterium amurskyense KMM 3673T 2. KJF4-6 (JQ800064) [98.9] Arthrobacter sulfureus DSM 20167T Phylum Proteobacteria Class Alphaproteobacteria 3. KJF12-15, KJF13-9, KJF8-9, KJF12-22, KJF15-13 [99.8], KJF12-23, KJF8-21, KJF14-2 [99.9], Loktanella salsilacus LMG 21507T KJF5-9, KJF6-15, KJF8-23, KJF9-18, KJF10-13, KJF12-14, KJF12-24, KJF14-25(JQ800254), KJF14-29, KJF15-11, KJF15-14, KJF15-21 [100] 4. KJF3-3(JQ800024), KJF3-16, KJF3-18, KJF3-36, KJF4-8 [96.0] Loktanella tamlensis SSW-35T 5. KJF14-12 (JQ800244) [95.7] Thalassobius gelatinovorus IAM 12617T 6. KJF8-4 (JQ800120) [99.2] Pacificibacter maritimus KMM 9031T 7. KJF6-16 (JQ800107) [98.0] Paracoccus seriniphilus MBT-A4T Class Betaproteobacteria 8. KJF12-10 (JQ800205) [97.6] Hydrogenophaga taeniospiralis ATCC 49743T Class Gammaproteobacteria 9. KJF14-10 [99.2], KJF2-19, KJF5-2 [99.3], KJF11-19, KJF14-17 [99.4], KJF2-3, KJF2-9, KJF2-13, Pseudoalteromonas arctica A37-1-2T KJF2-14, KJF2-15 KJF2-18, KJF3-21, KJF6-1, KJF6-12, KJF11-26, KJF15-3 [99.5], KJF6-14, KJF11-18 [99.6], KJF3-10, KJF13-15 (JQ800233) [99.7] KJF10-16, KJF11-24, KJF13-2, KJF14-4 [99.9], KJF1-18, KJF3-4, KJF3-5, KJF3-26, KJF5-6, KJF6-10,KJF6-11, KJF6-17, KJF11-17,KJF13- 13 [100] 10. KJF15-2 (JQ800260) [100] Pseudoalteromonas aliena KMM 3562T 11. KJF6-5 (JQ800097), KJF7-5 [99.8] Pseudoalteromonas elyakovii KMM 162T 12. KJF3-7 [99.8], KJF8-14,KJF9-4 (JQ800142) [99.9],KJF8-1 [100] Pseudoalteromonas nigrifaciens NCIMB 8614T 13. KJF4-1 [99.3], KJF3-24 (JQ800042) [100] Pseudoalteromonas paragorgicola KMM3548T 14. KJF3-6 [99.0], KJF5-11 [99.2], KJF5-7 [99.5], KJF3-31, KJF3-34, [99.6], KJF10-3 (JQ800159) [99.7] Pseudoalteromonas translucida KMM 520T 15. KJF8-12 [98.8],KJF8-5 (JQ800121) [99.2], Glaciecola mesophila KMM 241T 16. KJF8-3 [99.5],KJF8-10 (JQ800125) [99.4], KJF8-24 [92.9], Marinobacter psychrophilus 20041T 17. KJF10-4 (JQ800160) [98.9] Colwellia hornerae ACAM 607T Table S2 (Continued……)</p><p>S.No Strain number (Accession No.) [16S rRNA gene sequence similarity (%)] Closest Type Strain 18. KJF9-8 (JQ800146)[99.9] Colwellia maris ABE-1T 19. KJF11-9 [98.4],KJF11-10, KJF11-25,KJF14-6, KJF15-12 (JQ800268) [98.5], KJF6-3 [98.7], KJF11-2 Psychromonas arctica Pull 5.3T [98.9] 20. KJF10-6 [99.5],KJF11-5 (JQ800175) [99.6] Psychromonas marina 4-22T 21 KJF8-16 [98.8], KJF11-27, KJF13-12 [99.3], KJF8-2, KJF10-1, KJF11-21, KJF11-22 (JQ800191) Shewanella arctica IR12T [99.4] 22 KJF4-3, KJF4-18 [98.7] KJF1-12 [99.4], KJF2-4, KJF3-33 (JQ800051) [100] Shewanella livingstonensis LMG198866T 23. KJF14-11 [98.8], KJF1-13, KJF5-4, KJF5-8, [99.3], KJF1-2, KJF1-7, KJF1-9 (JQ799994), KJF3-15 Shewanella vesiculosa M7T [99.4], KJF12-17 [99.6], KJF10-15 [99.8],KJF11-12 [99.9], KJF9-10, KJF15-1, KJF15-15 [100] 24. KJF11-3 [99.6], KJF6-2 (JQ800094) [99.7] Shewanella gelidimarina ACAM 456T 25. KJF13-1 (JQ800220) [97.3] Shewanella pacifica KMM 3597T 26. KJF11-6 (JQ800176) [99.9] Pseudomonas arsenicoxydans VC-1T 27. KJF3-20,KJF3-35,KJF3-39 (JQ800057) [100] Pseudomonas guineae M8T 28. KJF12-13 (JQ800208),KJF12-16 [99.3], KJF12-5 [99.5] Psychrobacter aquimaris SW-210T 29. KJF1-10 [98.7], KJF1-6 (JQ799991) [99.0], Psychrobacter arenosus R7T 30. KJF2-8 (JQ800011) [99.7], KJF3-9 [99.9], KJF10-14 [100] Psychrobacter cryohalolentis K5T 31. KJF11-15 (JQ800184) [99.4] Psychrobacter okhotskensis MD17T 32. KJF14-27 (JQ800256) [99.5] Psychrobacter piscatorii T-3-2T 33. KJF1-5, KJF3-2 [99.5], KJF1-14, KJF3-25, KJF3-38, KJF4-14, KJF14-26, KJF14-28, KJF15-8 [99.6], Psychrobacter fozii NF23T KJF3-23, KJF3-32, KJF3-37 [99.7], KJF4-17,KJF12-12 [99.8], KJF1-17, KJF5-10, KJF11-28 (JQ800197) [99.9], KJF2-6, KJF3-1, KJF3-19, KJF3-22, KJF3-27, KJF3-30, KJF5-3, KJF6-9, KJF7- 10, KJF7-6, KJF9-2 [100] 34. KJF5-5 [99.0], KJF13-17 [99.1], KJF6-13, KJF9-1, KJF14-24 [99.2], KJF4-4, KJF4-11, KJF9-3, Marinomonas primoryensis KMM 3633T KJF9-14, KJF9-16, KJF9-17 [99.3], KJF3-28, KJF4-2, KJF12-7, KJF13-5, KJF13-14, KJF14-14 (JQ800246), KJF15-6 [99.4], KJF2-11 [99.6], KJF11-7 [99.8], KJF2-12, KJF4-5, KJF4-9, KJF4-10, KJF9-11, KJF9-15, KJF10-17, KJF11-23, KJF14-13 [99.9] Phylum Bacteroidetes Class Sphingobacteriia 35. KJF13-18 (JQ800236) [95.9],KJF12-19 [96.2] Pedobacter daechungensis Dae 13T Class Cytophagia 36. KJF5-15 (JQ800092) [99.1] Algoriphagus ratkowskyi LMG 21435T Table S2 (Continued……)</p><p>S.No Strain number (Accession No.) [16S rRNA gene sequence similarity (%)] Closest Type Strain Class Flavobacteriia 37. KJF10-2, KJF12-2 (JQ800199),KJF12-3 [97.5] Bizionia saleffrena HFDT 38. KJF15-18 [98.2], KJF9-12 [98.8], KJF8-20[98.9], KJF14-18,, KJF2-1[99.0], KJF4-12, KJF7-8, Flavobacterium degerlachei LMG 21915T KJF15-19, KJF15-20 [99.1], KJF8-19, KJF10-9, KJF10-12, KJF15-7 [99.2], KJF1-19, KJF2-7, KJF2- 17, KJF6-8, KJF12-18,KJF15-9 [99.4], KJF4-20 [99.5], KJF2-10 [99.6], KJF3-12, KJF3-40 (JQ800058), KJF14-22 [99.7], KJF6-7, KJF1-16 [99.9] 39. KJF12-9 [98.2], KJF1-4 [98.5], KJF3-13 (JQ800033) [99.5],KJF9-9 [99.7] Flavobacterium frigidarium A2iT 40. KJF2-2, KJF4-19, KJF5-13 [98.5], KJF14-19 [98.6], KJF7-11 [98.7],KJF2-5,KJF15-16 [98.8], KJF5- Flavobacterium frigoris LMG 21922T 14, KJF7-7, KJF10-11 [98.9] KJF7-9, KJF8-18, KJF9-13, KJF11-11, KJF11-13, KJF11-16, KJF11- 20, KJF12-11, KJF12-20, KJF12-21, KJF13-10 (JQ800228), KJF13-8, KJF14-20, [99.0], KJF1-1, KJF15-10 [99.1] KJF4-16, KJF10-10 [99.2], KJF14-21 [99.4] 41. KJF8-17 [96.2], KJF11-14 [96.3], KJF13-16, KJF15-17 [99.5], KJF13-11 [99.6], KJF13-7 Flavobacterium gelidilacus LMG 21477T (JQ800228) [100] 42 KJF4-15 (JQ800072) [98.8] Flavobacterium omnivorum JCM 11313T 43. KJF4-7 (JQ800065) [98.5] Flavobacterium psychrolimnae LMG 22018T 44. KJF14-1 (JQ800237) [99.3] Lacinutrix himadriensis E4-9aT 45. KJF3-29 (JQ800047),KJF8-13 [98.6] Maribacter arcticus KOPRI 20941T 46. KJF8-6 [97.7], KJF8-11 [98.1], KJF13-4 (JQ800223) [98.5], KJF6-4 [98.6], KJF12-1 [98.7], KJF7-3, Polaribacter butkevichii KMM 3938T KJF9-5, KJF12-6 [98.8], KJF13-3 [99.0] 47. KJF9-7 (JQ800145) [99.4] Polaribacter filamentus 215T 48. KJF3-11,KJF11-1 [98.2], KJF9-6 [98.6],KJF5-1 (JQ800078) [99.1] Polaribacter irgensii 23-PT 49. KJF14-3 (JQ800239) [99.5] Winogradskyella psychrotolerans RS-3T Table S3. Phylogenetic affiliation of the 43 bacterial isolates from Kongsfjorden sediments based on 16S rRNA gene sequence similarity</p><p>S.No Strain number (Accession No.) [16S rRNA gene sequence similarity (%)] Closest Type Strain Phylum Actinobacteria 1. FS14-4 (JQ799984) [99.2] Leifsonia rubra CMS 76rT 2. FS5-1 (JQ799950) [99.3],FS14-1 [99.4] Salinibacterium amurskyense KMM 3673T Phylum Proteobacteria Class Alphaproteobacteria 3. FS12-4 [97.3], FS3-2 (JQ799944) FS4-5 [97.4] ,FS12-3 [98.8], Sulfitobacter dongichola DSW25T 4. FS5-3 (JQ799952) [98.7] Sulfitobacter guttiformis Ekho Lake-38T 5. FS13-4 (JQ799978) [95.7], Thalassobius gelatinovorus IAM1261T 6 FS14-5 (JQ799985) [95.5] Planktotalea frisia SH6-1T Class Betaproteobacteria 7. FS13-1 (JQ799975) [96.5], FS10-3 [96.9], FS13-2 [98.3] Hydrogenophaga taeniospiralis ATCC 49743T Class Gammaproteobacteria 8. FS15-1 (JQ799986) [98.8] Colwellia aestuarii SMK-10T 9. FS12-2 (JQ799972) [98.9] Colwellia hornerae ACAM 607T 10. FS3-4, FS7-4 [99.7], FS4-2, FS6-2, FS6-4 (JQ799955), FS7-2, FS10-1 [99.9] Colwellia psychrerythraea 34HT 11. FS10-2 (JQ799964) [97.8] Marinobacterium rhizophilum CL-YJ9T 12. FS7-1 [98.5], FS11-1 (JQ799968) [98.6] Moritella abyssi 2693T 13. FS7-3 (JQ799958) [99.0] Moritella profunda 2674T 14. FS8-4 (JQ799962) [100] Photobacterium frigidiphilum SL13T 15. FS14-2 [99.3],FS14-3 (JQ799983) [98.2] Pseudomonas taeanensis MS-3T 16. FS5-4 (JQ799953) [99.1] Psychromonas arctica Pull 5.3T 17. FS11-3 (JQ799970) [98.6] Psychromonas ossibalaenae JAMM 0738T 18. FS10-4 [98.8],FS10-6 (JQ799967) [98.9] Shewanella abyssi C941T 19. FS8-1 [99.7], FS5-2 (JQ799951) [99.8], Shewanella benthica ATCC 43992T 20. FS4-1 (JQ799946), FS4-4, FS11-2 [98.8], FS8-2 [98.6] Shewanella gelidimarina ACAM 456T 21. FS13-5 (JQ799979) [100], FS13-6 [99.9] Shewanella livingstonensis LMG198866T 22. FS13-3 (JQ799977) [100.0], Shewanella vesiculosa M7T Phylum Bacteroidetes Class Flavobacteria 23. FS12-1 (JQ799971) [97.8] Polaribacter glomeratus ATCC 43844T Table S4. Growth characteristics and enzymatic activities of representative phylotypes from Kongsfjorden water and sediment samples </p><p>Isolate Growth Enzymatic activities* Code Characteristics Temp pH NaCl Am Lip Cas U Gel DNase (°C) (M) 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C KJF1-9 4-18 6-10 1.0 - - + + + + + + + + + + KJF2-8 4-30 7-10 1.0 - - + + ------KJF3-3 4-30 6-9 1.0 + + + + + + - - - - + + KJF3-13 4-30 7-10 0.5 - - + + ------KJF3-39 4-25 6 -9 1.0 - - + + ------KJF3-40 4-25 5-9 2.0 + + ------KJF4-6 4-18 6-10 2.5 - - - - + + ------KJF4-15 4-18 6-8 0.3 - - - - + - - - - - + - KJF5-1 4-18 6-9 1.0 - - + + ------KJF5-15 4-25 6-9 1.0 - - - - + + - - - - + + KJF6-2 4-30 5-9 1.5 ------KJF6-16 4-25 6-10 0.5 - - + + + + + + + + + + KJF8-4 4-30 5-9 2.0 - - + + ------KJF8-7 4-30 5-10 0.5 ------+w +w +w +w +w +w KJF8-10 4-25 6 -9 1.5 ------KJF9-8 4-18 7-10 1.0 - - + + ------+w + KJF10-3 4-25 7-9 1.5 - - + + ------KJF11-5 4-18 7-10 1.5 ------KJF11-15 4-30 6 -10 1.5 - - + + ------KJF11-28 4-18 6-10 2.0 - - + + + + + + + + + + KJF12-2 4-18 7-10 1.0 - - - - + + - - - - + + Table S4. Continued.....</p><p>Isolate Growth Enzymatic activities* Code Characteristics Temp pH Am Lip Cas U Gel DNase (°C) 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C KJF12-10 4-25 6-8 +w + ------+ + KJF12-13 4-18 6-9 - - + + ------KJF13-1 4-30 6-9 - - +w + ------KJF13-4 4-18 7-9 ------KJF13-15 4-30 6-10 + + + + + + - - - - + + KJF13-18 4-25 6-8 +w +w + + + + + + + + + + KJF14-3 4-30 5-9 - - + + + +w - - - - + + KJF14-12 4-18 6-9 - - +w +w ------KJF14-14 4-18 7-10 ------KJF14-27 4-25 6 -9 - - + + ------KJF15-12 4-18 7-10 + + ------KJF1-6 4-37 5-9 ------KJF9-4 4-30 5-9 - - + + ------+ +w KJF13-7 4-30 5-9 ------FS3-2 4-30 6-9 - - - + - - - + - - - - FS6-4 4-18 6-8 + + ------FS5-1 4-30 5-8 + + ------w+ w+ - - FS5-3 4-30 5-9 + + - + - + + + - + - - FS8-4 4-18 5-8 + + + + - + - - + + + - FS10-2 4-30 6-8 - - + ------w+ - -</p><p>Table S4. Continued.... Isolate Growth Enzymatic activities* Code Characteristics Temp NaCl Am Lip Cas U Gel DNase (°C) (M) 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C 4°C 18°C FS11-1 4-30 0.5 w+ - + + - - - - - + - - FS11-3 4-30 0.5 - + ------w+ - - - FS12-2 4-18 0.5 - - - + - - - - + + - - FS13-1 4-30 0.5 - - - + ------FS14-4 4-30 1.0 + + - + ------FS4-1 4-18 0.3 - + - + - - - - + + + + FS13-5 4-25 0.3 w+ - - - + - - - + + - - FS13-4 4-30 1.5 + - - - - - + - - - - - FS14-3 4-30 1.0 + - - - - - + + - + - - FS15-1 4-18 0.5 - - + - + + - - + + - - FS5-4 4-18 0.5 - - - + - - + + + + - - Note: (w+) = weak positive, (+) = positive, (-) = negative. Am = amylase, Lip = lipase, Cas =caseinase, U = urease, Gel = gelatinase, (*) = all phylotypes were negative for chitinase activity. Table S5. Pigment characteristics of isolates from water and sediment samples of Kongsfjorden </p><p>S. No. Strain No. Colony colour Absorption maxima (nm)</p><p>1 KJF1-9 Orange 330 2 KJF3-3 Yellow 245 3 KJF3-13 Yellow 230 4 KJF3-16 Yellow 230 5 KJF3-20 Yellow 230 6 KJF4-6 Yellow 240 7 KJF5-1 Yellow 245 8 KJF5-15 Orange 420 9 KJF8-7 Yellow N 10 KJF10-10 Lemon yellow 205 11 KJF10-12 Dark yellow N 12 KJF12-14 Peach 245 13 KJF13-2 Brown 450,480(s),335 14 KJF13-10 Yellow 350 15 KJF14-1 Brown N 16 KJF14-4 Yellow 450,480(s),335 17 KJF14-13 White 245 18 KJF14-15 Yellow 245 19 KJF14-18 Orange 350 20 KJF15-3 Yellow 450,480(s),335 21 KJF15-14 Yellow 215 22 FS4-1 Orange red 295, 415 23 FS5-1 Yellow 285, 445 24 FS6-4 Pale pink-brown 295 25 FS13-2 Pale pink-brown 285 26 FS14-1 Yellow 255 27 FS14-4 Yellow 255, 325, 475 28 FS14-5 Reddish brown 330, 405 29 FS15-4 Yellow 275 30 FS16-1 Cream-brown 415</p><p>Note: (s) =shoulder peak, N= No Peaks</p><p>Fig. S1. A maximum likelihood phylogenetic tree showing the evolutionary history of KJF phylotypes. The alphaproteobacterial and flavobacterial clades have been condensed. </p><p>Fig S2. Expanded tree of the alphaproteobacterial clade from Fig S1 Fig S3. Expanded tree of the flavobacterial clade in Fig S1</p><p>Fig S4. A maximum-likelihood phylogenetic tree showing the evolutionary history of FS phylotypes.</p>