<p> Additional file 5</p><p>Table S1: Information on ISH probes</p><p>Gene Remarks Gene Name Forward primer Vector Restriction RNA symbol (Genbank accession Synonyms Reverse primer enzyme for polymerase number) AS probe for AS probe Arc (AF162777) activity regulated 5’-CGCAGCACCGACGACCAGAT-3’ p123T BamHI T7 cytoskeletal-associated 5’-GACCAGGGCAGACAGATGAG-3’ protein Arc3.1 c-fos EST clone FBJ osteosarcoma None pCMV-SPORT6 EcoRI T7 (BC029814) oncogene Fos, cFos c-jun EST clone Jun oncogene None pCMV-SPORT6 EcoRV T7 (BC094032) Jun, Junc Egr1 EST clone early growth response 1 None pCMV-SPORT6 EcoRI T7 (NM_007913) Egr-1, Krox-1, Krox-24, Zif268 Egr3 (NM_018781) early growth response 3 5’-CAATCTGTACCCCGAGGAGA-3’ p123T BamHI T7 Pilot 5’-CCACAGAACTCACAGGCAAA-3’ Fosb (NM_008036) FBJ osteosarcoma 5’-CGGTCTCGGGGAAATGCCCG-3’ pCR II EcoRV Sp6 oncogene B 5’-GCGAGTTCAGCGGGTCGGAC-3’ Jun-B EST clone Jun-B oncogene None pCMV-SPORT6 EcoRV T7 (BC003790) Nor1 A kind gift from Dr nuclear receptor See ref. pBluescript SK+ HindIII T3 Levesque, Université de subfamily 4, group A, Montréal member 3 Ref. J Pharmacol Exp Ther Nr4a3, NOR-1 313: 460-473, 2005. (NM_015743) Npas4 (NM_153553) neuronal PAS domain 5’-TCTCACTGTGCGCCAGCAGC-3’ pCR II BamHI T7 protein 4 5’-CCACGCCCTGAGCCAACTGG-3’ Nxf Nr4a1 (NM_010444) nuclear receptor 5’-GCGGAACCGCTGCCAGTTCT-3’ pCR II BamHI T7 subfamily 4, group A, 5’-GTTGGAGGCTCGCCCAGCTG-3’ member 1 NGFI-B, Nur77 Pde2 A kind gift from Dr. phosphodiesterase 2A, See ref. pcDNA3 HindIII Sp6 (Pde2a) Joseph Beavo, cGMP-stimulated University of Washington CGS-PDE; cGSPDE Ref. PNAS 94: 3388-3395, 1997 (NM_001008548) Th (NM_009377.1) tyrosine hydroxylase 5’-TGTCACGTCCCCAAGGTTCA-3’ p123T BamHI T7 5’-CCAAGAGCAGCCCATCAAAG-3’</p>