<p>Titles and legends to supplementary figures</p><p>Supplemental Figure S1. Apoptotic assays in LN18 and LN428 cells after various doses of IR exposure. (A) Caspase 3/7 activity was measured at indicated days after various doses of IR exposure. (B) Western blot analyses of levels of pro-caspase 3 and cleaved PARP. The proteins were detected using each specific antibody at 2 and 3 days after various doses of IR exposure. MCF7 cells treated with 10 g/ml doxorubicin for 2 days were used as a positive control (PC) for cleaved PARP. Quantitative results are presented as mean  standard deviation (SD) of three independent experiments. Significantly different: * P < 0.01 versus C at indicated days.</p><p>Supplemental Figure S2. PTEN status also influences on cellular responses to IR in human prostate cancer cells. PTEN-deficient PC-3 or PTEN-proficient DU 145 cell lines were exposed to IR at 8 or 10 Gy. (A) Morphologic changes were observed at 2 or 4 days after IR exposure. (B) Trypan blue exclusion assay at 2 days after irradiation. (C) Western blot analyses were performed using specific antibodies indicated at 2 days after irradiation.</p><p>Actin was detected as a loading control. Each bar in the graphs indicates mean  SD of three independent experiments. Significantly different: * P < 0.01 versus C.</p><p>Supplemental Figure S3. Apoptotic assays in glioma cells after treatment of doxorubicin. (A) Caspase 3/7 activity was assessed after exposure to doxorubicin (10 or 20</p><p>g/ml) for 10 and 24 hours. (B) Western blot analyses of levels of pro-caspase 3 and cleaved</p><p>PARP. The proteins were detected using each specific antibody at 10 and 24 hours after treatment of doxorubicin (10 or 20 g/ml). Each bar in the graphs indicates mean  SD of three independent experiments. Significantly different: * P < 0.01 versus C. </p><p>1 Supplemental Figure S4. Cellular responses in U87 and LN18 cells treated with H2O2.</p><p>Cellular morphology was observed under phase contrast microscopy (A), dead cells were quantified using trypan blue exclusion assay (B), and Western blot analyses were performed</p><p>(C) in U87 and LN18 cells which were treated with 200 or 400 μM of H2O2 for 2 days. Actin was detected as a loading control. Each bar in the graphs indicates mean  SD of three independent experiments. Significantly different: * P < 0.01 versus C.</p><p>2</p>