Titles and legends to supplementary figures
Supplemental Figure S1. Apoptotic assays in LN18 and LN428 cells after various doses of IR exposure. (A) Caspase 3/7 activity was measured at indicated days after various doses of IR exposure. (B) Western blot analyses of levels of pro-caspase 3 and cleaved PARP. The proteins were detected using each specific antibody at 2 and 3 days after various doses of IR exposure. MCF7 cells treated with 10 g/ml doxorubicin for 2 days were used as a positive control (PC) for cleaved PARP. Quantitative results are presented as mean standard deviation (SD) of three independent experiments. Significantly different: * P < 0.01 versus C at indicated days.
Supplemental Figure S2. PTEN status also influences on cellular responses to IR in human prostate cancer cells. PTEN-deficient PC-3 or PTEN-proficient DU 145 cell lines were exposed to IR at 8 or 10 Gy. (A) Morphologic changes were observed at 2 or 4 days after IR exposure. (B) Trypan blue exclusion assay at 2 days after irradiation. (C) Western blot analyses were performed using specific antibodies indicated at 2 days after irradiation.
Actin was detected as a loading control. Each bar in the graphs indicates mean SD of three independent experiments. Significantly different: * P < 0.01 versus C.
Supplemental Figure S3. Apoptotic assays in glioma cells after treatment of doxorubicin. (A) Caspase 3/7 activity was assessed after exposure to doxorubicin (10 or 20
g/ml) for 10 and 24 hours. (B) Western blot analyses of levels of pro-caspase 3 and cleaved
PARP. The proteins were detected using each specific antibody at 10 and 24 hours after treatment of doxorubicin (10 or 20 g/ml). Each bar in the graphs indicates mean SD of three independent experiments. Significantly different: * P < 0.01 versus C.
1 Supplemental Figure S4. Cellular responses in U87 and LN18 cells treated with H2O2.
Cellular morphology was observed under phase contrast microscopy (A), dead cells were quantified using trypan blue exclusion assay (B), and Western blot analyses were performed
(C) in U87 and LN18 cells which were treated with 200 or 400 μM of H2O2 for 2 days. Actin was detected as a loading control. Each bar in the graphs indicates mean SD of three independent experiments. Significantly different: * P < 0.01 versus C.
2