<p>Toxicological impact of Amaranth, Sunset Yellow and Curcumin as Food Coloring Agents in Albino Rats.</p><p>Mohamed M.Hashem1, Attia H.Atta1*, Mahmoud S. Arbid2, Somaia A. Nada2, Samar M. Mouneir1 and Gihan Farag Asaad2 </p><p>.Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt 1 .Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Qassim University, Buraydah, KSA *1 ..Department of Pharmacology, National Research Center, Dokki, Giza, Egypt 2 Abstract</p><p>© J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 1 © J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 2 © J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 3 Groups Glutathione Lipid Peroxide Liver Kidney Liver Control 8.6±0.27 5.44±0.16 132 ±9.82 Amaranth (ADI) 7.13±0.31 5.51±0.09 141.89±8.98 Amaranth (10 xADI) 7.42±0.49 5.84±0.07 115.17±2.99 Sunset Yellow ADI 7.48±0.13 5.6±0.07 113.57±2.94 Sunset Yellow (10 xADI) 7.63±0.23 5.85±0.18 137±9.81 Curcumin ADI 8.5±0.84 5.7±0.17 107±3.66* Curcumin (10xADI) 9.23±0.31 5.84±0.23 73.5±3.61* Table 1: Effect of administration of amaranth, sunset yellow and curcumin in doses of ADI and 10 times ADI daily for 2 months on liver and kidney glutathione (umol/gram tissue) and lipid peroxide (</p><p>Groups ALT AST Alkaline Phosphatase Control 33.24±1.07 5 9 . 9 ± 1 . 2 110.8±2.33</p><p>Amaranth (ADI) 36.51±2.14 6 0 . 9 8 ± 1 . 4 1 116.82±3.03 Amaranth (10 xADI) 37.65±2.16 6 4 . 4 8 ± 7 . 0 3 126.73±2.74* Sunset Yellow ADI 36.86±2.59 5 9 . 5 6 ± 1 . 6 4 117.31±1.58 Sunset Yellow (10 xADI) 40.74±1.64* 6 5 . 5 2 ± 1 . 6 4 * 117.05±2.33 Curcumin ADI 38.25±1.62 5 9 . 7 3 ± 0 . 6 9 110.84±2.13 Curcumin (10 xADI) 37.78±2.33 6 1 . 7 4 ± 2 . 5 2 111.88±2.82 Table 2: Effect of amaranth, sunset yellow and curcumin in doses of ADI and 10 times ADI daily for 2 months on liver and kidney function tests in albino rats (mean ± SE, n=10). </p><p>Groups Hb content RBCs count PCV % MCV (fl=10- MCH MCHC % WBCs g/dl million/mm3 15) (pg=1012) count</p><p>© J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 4 103/mm3</p><p>Control 14.31±0.81 5.68±0.33 30.68±1.5 54.45±2.76 25.53±1.91 46.93±2.54 11.2+0.39</p><p>Amaranth ADI 14.24±0.22 5.64±0.27 30.00±0.77 53.71±2.39 25.53±1.17 47.60±1.25 11.83+0.73</p><p>Amaranth 10XADI 13.97±0.94 5.04±0.4 28.67±1.98 57.51±3.35 28.01±1.59 48.75±0.7 12.32+0.42</p><p>Sunset Yellow ADI 13.58±0.82 5.71±0.28 28.00±1.19 49.16±1.15 23.86±1.33 48.46±1.97 11.7+0.84</p><p>Sunset yellow 14.14±0.7 5.08±0.24 25.17±1.38* 50.26±4.10 28.21±2.19 37.53±1.05 9.54+0.54* 10XADI * Curcumin ADI 12.92±0.69 5.55±0.4 28.00±0.99* 49.34±3.9 24.19±2.65 47.27±1.98 11.22+0.52</p><p>Curcumin 10XADI 12.96±0.5 5.31±0.37 28.00±0.78* 54.30±3.54 25.08±1.59 46.63±2.41 9.93+0.58</p><p>Table 3: Effect of amaranth, sunset yellow and curcumin in doses of ADI and 10 times ADI daily for 2 months on blood picture of albino rats (mean ± SE, n=10)</p><p>Drug No of Total No. of Growth Skeletal Visceral Total Pregnant foeti retardation abnormalities abnormalities abnormalities Dams No. of foeti % No. of foeti % No. of % No. of foeti % foeti Control 6 55 0 0 0 0 0 0 0 0 1 ml Distilled Water/rat Amaranth 6 36 10 27.8 9 25 3 8.3 22 61.1 47 mg/kg bwt Sunset Yellow 6 58 6 10.3 0 0 3 5.1 9 15.5 315 mg/kg bwt 7 Curcumin 6 63 2 3.2 0 0 0 0 2 3.1 157.5 mg/kg bwt Table 4: Effect of amaranth, sunset yellow and curcumin in doses of 47, 315 and 157.5 mg/kg bwt respectively (10X ADI) from day 6 to day 15 on foetal abnormalities in albino rats (n=6 ).</p><p>© J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 5 © J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 6 © J of Pak Med St. www.jpmsonline.com Volume 1, Issue 2. July-September, 2011. Page | 7 </p>