US010508291B2 United States Patent ( 10 ) Patent No.: US 10,508,291 B2 Medoff (45 ) Date of Patent : * Dec . 17 , 2019 ( 54 ) PROCESSING BIOMASS C12P 19/02 (2006.01 ) C12P 19/14 ( 2006.01) (71 ) Applicant: Xyleco, Inc. , Wakefield , MA (US ) C12P 710 (2006.01 ) C12N 9/00 (2006.01 ) ( 72 ) Inventor: Marshall Medoff , Wakefield , MA (US ) A23K 50/75 ( 2016.01) A23K 50/30 ( 2016.01) ( 73 ) Assignee : XYLECO , INC . , Wakefield , MA (US ) A23K 50/40 (2016.01 ) (52 ) U.S. CI. ( * ) Notice : Subject to any disclaimer , the term of this CPC C12P 7762 (2013.01 ) ; A23K 10/00 patent is extended or adjusted under 35 ( 2016.05 ) ; A23K 10/12 ( 2016.05 ) ; A23K 10/30 U.S.C. 154 (b ) by 0 days . ( 2016.05 ) ; A23K 10/37 ( 2016.05 ) ; A23K 20/163 ( 2016.05 ) ; A23K 20/189 ( 2016.05 ) ; This patent is subject to a terminal dis A23K 40/10 ( 2016.05 ) ; A23K 50/10 ( 2016.05 ) ; claimer . A23K 50/80 ( 2016.05) ; A23L 5/00 (2016.08 ) ; A23L 5/30 ( 2016.08 ) ; A61K 8/97 ( 2013.01 ) ; ( 21) Appl. No .: 15 /657,013 A61Q 19/00 ( 2013.01 ) ; B01D 39/18 ( 2013.01) ; C08H 8/00 ( 2013.01 ) ; C08J 3/28 (22 ) Filed : Jul, 21 , 2017 (2013.01 ) ; C08L 1/02 ( 2013.01 ) ; C08L 5/14 (2013.01 ) ; C12P 7/10 ( 2013.01) ; C12P 7/42 (65 ) Prior Publication Data ( 2013.01 ); C12P 13/04 ( 2013.01 ) ; C12P 19/02 US 2017/0321233 A1 ( 2013.01) ; C12P 19/14 ( 2013.01) ; C12P 19/44 Nov. 9 , 2017 ( 2013.01 ); C13K 1/02 ( 2013.01 ); D21C 5/005 (2013.01 ) ; D21C 9/001 (2013.01 ) ; A23K 50/30 Related U.S. Application Data ( 2016.05 ) ; A23K 50/40 (2016.05 ) ; A23K 50/75 (2016.05 ) ; A23V 2002/00 (2013.01 ) ; C12N (60 ) Division of application No. 15 /040,318 , filed on Feb. 9/00 ( 2013.01) ; C12P 2201/00 (2013.01 ) ; 10 , 2016 , now Pat. No. 9,745,604, which is a C12P 2203/00 (2013.01 ) ; YO2E 50/10 continuation of application No. 14 /813,898 , filed on ( 2013.01) ; YO2E 50/16 (2013.01 ) ; YO2E 50/17 Jul. 30 , 2015 , now Pat . No. 9,803,222 , which is a ( 2013.01) ; YO2E 50/30 (2013.01 ) ; YO2P continuation of application No. 14 / 750,995 , filed on 60/877 (2015.11 ) Jun . 25 , 2015 , now Pat. No. 9,745,609 , which is a (58 ) Field of Classification Search continuation of application No. 14 / 333,675 , filed on CPC .... COSH 8/00 ; C12P 2201/00 ; C12P 2203/00 ; Jul. 17 , 2014 , now Pat . No. 9,309,545 , which is a C13K 1/02 , A23K 1/001 ; A23K 1/1643 ; continuation of application No. 13 /902,246 , filed on A23K 1/1813 ; A23K 1/188 ; A23L May 24 , 2013 , now Pat. No. 8,877,472 , which is a 1/0257 ; A61K 8/97 ; D21C 5/005 continuation of application No. 12/ 417,900 , filed on See application file for complete search history . Apr. 3 , 2009 , now abandoned . (Continued ) (56 ) References Cited (51 ) Int. Cl. U.S. PATENT DOCUMENTS A23L 5/30 ( 2016.01) 1,920,129 A 7/1933 Munroe et al. A23L 5/00 (2016.01 ) 3,352,773 A 11/1967 Schwartz et al . A23K 40/10 ( 2016.01 ) (Continued ) A61K 8/97 ( 2017.01 ) B01D 39/18 ( 2006.01 ) FOREIGN PATENT DOCUMENTS C08H 800 (2010.01 ) C08J 3/28 ( 2006.01 ) CA 2809329 6/2008 C12P 19/44 (2006.01 ) CN 101041834 A 9/2007 CO8L 1/02 ( 2006.01 ) ( Continued ) C08L 5/14 ( 2006.01 ) D21C 5/00 (2006.01 ) OTHER PUBLICATIONS D21C 9/00 ( 2006.01) A23K 10/12 ( 2016.01 ) Adams, “ Corn Stover as Feed for Cattle, ” Penn State College of A23K 10/30 (2016.01 ) Agricultural Sciences , Penn State Extension , Feb. 1 , 2002 ( 2 pages ) . A23K 10/37 ( 2016.01) (Continued ) A23K 20/163 ( 2016.01 ) A23K 20/189 ( 2016.01 ) Primary Examiner Aaron J Kosar C12P 7762 ( 2006.01) (74 ) Attorney , Agent, or Firm -Lando & Anastasi , LLP A61Q 19/00 ( 2006.01 ) C12P 13/04 ( 2006.01) (57 ) ABSTRACT A23K 50/10 ( 2016.01) Biomass ( e.g., plant biomass , animal biomass, microbial, A23K 50/80 ( 2016.01 ) and municipal waste biomass ) is processed to produce useful C13K 1/02 ( 2006.01 ) products , such as food products and amino acids . C12P 742 ( 2006.01 ) A23K 10/00 ( 2016.01 ) 15 Claims, 54 Drawing Sheets US 10,508,291 B2 Page 2 Related U.S. Application Data 2009/0286295 Al 11/2009 Medoff et al . 2010/0087687 A1 4/2010 Medoff 2010/0093241 A1 4/2010 Medoff (60 ) Provisional application No.61 / 139,453 , filed on Dec. 2010/0105119 A1 4/2010 Medoff 19, 2008 , provisional application No. 61/ 073,674 , 2010/0108567 Al 5/2010 Medoff filed on Jun . 18 , 2008, provisional application No. 2010/0124583 A1 5/2010 Medoff 61 / 049,405 , filed on Apr. 30 , 2008 . 2010/0179315 A1 7/2010 Medoff 2010/0200806 A1 8/2010 Medoff et al . References Cited 2010/0203495 Al 8/2010 Medoff et al . ( 56 ) 2010/0206501 A1 8/2010 Medoff 2010/0297720 A1 11/2010 Medoff et al . U.S. PATENT DOCUMENTS 2010/0304440 A1 12/2010 Medoff 3,939,286 A 2/1976 Jelks 2011/0011960 A1 1/2011 Medoff 4,268,505 A 5/1981 Yoshikumi et al. 2011/0027837 A1 2/2011 Medoff 4,274,163 A 6/1981 Malcom et al. 2011/0039317 Al 2/2011 Medoff 4,275,163 A 6/1981 Gallo 2011/0081335 A1 4/2011 Medoff 4,321,328 A 3/1982 Hoge 2011/0081336 A1 4/2011 Medoff 4,431,675 A 2/1984 Schroeder et al. 2011/0111456 A1 5/2011 Medoff 4,769,082 A 9/1988 Kumakura et al . 2011/0139383 Al 6/2011 Medoff 5,122,598 A 6/1992 della Valle et al. 2011/0155559 Al 6/2011 Medoff 5,221,357 A 6/1993 Brink 2011/0262985 Al 10/2011 Medoff 5,314,978 A 5/1994 Kim et al. 2011/0265991 A1 11/2011 Medoff 5,417,824 A 5/1995 Greenbaum 2011/0287498 A1 11/2011 Medoff et al. 5,538,730 A 7/1996 Romeo et al. 2012/0003704 Al 1/2012 Medoff 5,753,474 A 5/1998 Ramey 2012/0052536 A1 3/2012 Medoff et al . 6,011,008 A 1/2000 Domb et al . 2012/0074337 A1 3/2012 Medoff 6,334,347 B1 1/2002 Iscla 2012/0077247 Al 3/2012 Medoff 6,344,347 B1 2/2002 Kino et al. 2012/0094358 A1 4/2012 Medoff 6,534,104 B1 3/2003 DeRouchey et al. 2012/0100577 A1 4/2012 Medoff et al . 6,555,335 B1 4/2003 Saloheimo et al . 2012/0100586 A1 4/2012 Medoff et al . 6,555,350 B2 4/2003 Ahring et al. 2012/0142065 A1 6/2012 Medoff 6,620,605 B2 9/2003 Fowler et al. 2012/0142068 A1 6/2012 Medoff 6,808,600 B2 10/2004 Ross et al. 2012/0315675 Al 12/2012 Medoff et al . 6,942,973 B2 9/2005 Yaver et al . 2013/0295624 Al 11/2013 Medoff et al . 7,005,508 B2 2/2006 Benedini et al . 2013/0302839 Al 11/2013 Harenberg et al . 7,408,056 B2 8/2008 Medoff et al. 2015/0342224 Al 12/2015 Medoff 7,846,295 B1 12/2010 Medoff 2015/0353974 A1 12/2015 Medoff 7,867,358 B2 1/2011 Medoff 7,867,359 B2 1/2011 Medoff FOREIGN PATENT DOCUMENTS 7,900,857 B2 3/2011 Medoff 7,931,784 B2 4/2011 Medoff EP 2276795 1/2011 7,932,065 B2 4/2011 Medoff IN 200200944 1/2005 7,935,219 B2 5/2011 Medoff IN 200401988 8/2006 8,003,368 B2 8/2011 Marchenko et al . JP S5971700 A 4/1984 8,052,838 B2 11/2011 Medoff JP S59113856 A 6/1984 8,070,912 B2 12/2011 Medoff KR 2002091479 12/2002 8,083,906 B2 12/2011 Medoff RU 2030155 C1 3/1995 8,142,620 B2 3/2012 Medoff SU 1262950 5/1990 8,147,655 B2 4/2012 Medoff WO WO -01 / 15689 3/2001 8,168,038 B2 5/2012 Medoff WO WO - 2001052620 7/2001 8,212,087 B2 7/2012 Medoff WO WO - 2001079241 10/2001 8,221,585 B2 7/2012 Medoff WO WO - 2006032282 3/2006 8,236,535 B2 8/2012 Medoff et al. WO WO - 2008073186 6/2008 8,377,668 B2 2/2013 Medoff et al. WO WO - 2009134791 11/2009 8,418,944 B2 4/2013 Medoff 8,454,803 B2 6/2013 Medoff 8,492,128 B2 7/2013 Medoff OTHER PUBLICATIONS 8,597,921 B2 12/2013 Medoff 8,603,787 B2 12/2013 Medoff Allen et al. , “ Fractionation of Sugar Cane with Hot , Compressed , 8,709,771 B2 4/2014 Medoff et al. Liquid Water, ” Industrial & Engineering Chemistry Research , 35 ( 8 ) , 8,835,142 B2 * 9/2014 Medoff A61K 8/97 pp . 2709-2715 ( 1996 ) . 435/171 8,841,101 B2 9/2014 Medoff Atchison et al ., " Subcontractor Report : Innovative Methods for 8,852,905 B2 10/2014 Medoff Corn Stover Collecting, Handling, Storing and Transporting — Mar. 8,877,472 B2 11/2014 Medoff 2003, ” National Renewable Energy Laboratory , NREL /SR - 510 8,945,245 B2 2/2015 Bals et al . 33893 , 63 pages (2004 ) . 9,078,461 B2 * 7/2015 Medoff A61K 8/97 Awafo et al. , “ Effect of Irradiation , as a Pretreatment, on Bioconver 9,278,896 B1 * 3/2016 Medoff BO1J 19/085 sion of Corn Stover into Protein -Rich Mycelial Biomass of Pluerotus 9,309,545 B2 4/2016 Medoff Sajor- Caju ,” Radiation Physics and Chemistry , 46 (4-6 ) pp .