Product datasheet Anti-ULBP1 Antibody Catalog Number: A04323-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-ULBP1 Antibody Gene Name ULBP1 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA Immunogen E.coli-derived human ULBP1 recombinant protein (Position: W27-K209). Purification Immunogen affinity purified. Observed MW 28KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P) 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information UL16 binding protein 1 (ULBP1) is a cell surface glycoprotein encoded by ULBP1 gene located on the chromosome 6. The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene. Reference Anti-ULBP1 Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-ULBP1 Antibody Catalog Number: A04323-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot Figure 2.IHC analysis analysis of anti- ULBP1 using anti- ULBP1 antibody (A04323-1).The antibody (A04323-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human mammary cancer conditions.Lane 1: Rat tissue. Biotinylated goat lung tissue lysates,Lane 2: anti-rabbit IgG was used Mouse brain tissue as secondary antibody. lysates,Lane 3: Mouse The tissue section was heart tissue lysates,Lane developed using Strepavi 4: Mouse lung tissue din-Biotin-Complex lysates,Lane 5: human (SABC) (Catalog # hepg2 whole cell SA1022) with DAB as the lysates,Use rabbit anti- chromogen. ULBP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ULBP1 at approximately 28KD. The expected band size for ULBP1 is at 28KD. Figure 3.IHC analysis Figure 4.Flow cytometry using anti- ULBP1 analysis of PC-3 antibody (A04323-1). cell(1x106) DyLight 488 detected in paraffin- conjugated goat anti- embedded section of rabbit IgG(blue) was used human mammary cancer as secondary tissue. Biotinylated goat antibody.Isotype control anti-rabbit IgG was used antibody (Green line) was as secondary antibody. rabbit IgG DyLight 488. The tissue section was Unlabelled sample (Red developed using Strepavi line). din-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Powered by TCPDF (www.tcpdf.org).