ELISA kits and monoclonal antibodies for THE COMPLEMENT SYSTEM MBL Classical Pathway Lectin Pathway Alternative Pathway H-Ficolin KIT 029 RIG 334 HYB 131 M-Ficolin ABS 036 C1-INH HYB 288 L-Ficolin C1q MBL/Ficolin ABS 005 C1q B H2O C3 (H2O) Factor B C1s C1s C1r MASP-2 D ABS 002 MASP-1 HAV 005 C3 C3 (H O) B 2 Factor D Carbohydrate Carbohydrate GAU 010 C2 GAU 008 HYB 050 C2 C4 C3 (H2O) Bb Ba Factor Ba/b HYB 008 C3 C3a Properdin (Factor P) HAV 004 C2b C4a HYB 005 Properdin HYB 0393 C3d C4b2a HAV 003 C2a C4b HYB 030 C3bBb C4 HYB 162-04 C3a/C3a (desArg) C3 GAU 013 C3a C3a C4b GAU 017 HYB 162-02 C5 C4b2a3b C3bBb3b HYB 029 C5 - C9 C5a/C5a (desArg) Membrane attack complex GAU 025 Factor H HYB 268 GAU 018 C9 GAU 020 ABS 004 Lysis or phagocytosis of the microorganism C5b-9 Factor I DIA 011 HYB 061 Overview of the complement system. The name of the component and BioPorto Diagnostics' catalog number are shown in the blue boxes. REFERENCES IN WHICH ANTIBODYSHOP PRODUCTS HAVE BEEN USED C2 Cat. no. HYB 050 ∙ Rooijakkers SH, Ruyken M, Roos A, Daha MR, Presanis JS, Sim RB, van Wamel WJ, van Kessel KP, van Strijp JA (2005) Immune eva- sion by a staphylococcal complement inhibitor that acts on C3 convertases. Nat Immunol 6:920-7. ∙ Oh KS, Kweon MH, Rhee KH, Ho Lee K, Sung HC (2003) Inhibition of complement activation by recombinant Sh-CRIT-ed1 analogues. Immunology 110:73-9. ∙ Laich A, Moffatt B, Wong KHN, Hickling TP, Koch C, Sim RB (2001) Purifi cation of second component of human complement, C2, by antibody affi nity chromatography. Intern J Bio-Chromatography 6:151-162. ∙ Laich A, Sim RB (2001) Complement C4bC2 complex formation: an investigation by surface plasmon resonance. Biochim Biophys Acta 1544:96-112. ∙ Stenbaek EI, Koch C, Barkholt V, Welinder KG (1986) Human complement component C2: production and characterization of poly- clonal and monoclonal antibodies against C2. Mol Immunol 23:879-886. C3 Cat. no. HAV 003 and HAV 004 ∙ Tang S, Zhou W, Sheerin NS, Vaughan RW, Sacks SH (1999) Contribution of renal secreted complement C3 to the circulating pool in humans. J Immunol 162:4336-41. ∙ Naughton MA, Botto M, Carter MJ, Alexander GJ, Goldman JM, Walport MJ (1996) Extrahepatic secreted complement C3 contributes to circulating C3 levels in humans. J Immunol 156:3051-6. ∙ Botto M, Fong KY, So AK, Koch C, Walport MJ (1990) Molecular basis of pol ymorphisms of human complement component C3. J Exp Med 172:1011-1017. ∙ Behrendt N, Hansen OC, Ploug M, Barkholt V, Koch C (1987) Localization and functional signifi cance of a polymorphic determinant in the third component of human complement. Mol Immunol 24:1097-1103. ∙ Koch C, Behrendt N (1986) A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody. Immunogenetics 23:322-325. C3a/C3a (desArg) Cat. no. GAU 013-16 and GAU 017-01 ∙ Lhotta K, Würzner R, Kronenberg F, Oppermann M, König P (1998) Rapid activation of the complement system by cuprophane de- pends on complemet component C4. Kidney Int 53:1044-1051. ∙ Ammon HPT, Ege W, Oppermann M, Göpel W, Eisele S (1995) Improvement in the long-term stability of an amperometric glucose sen- sor system by introducing a cellulose membrane of bacterial origin. Anal Chem 67:466-471. ∙ Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol Immunol 30:935-940. ∙ Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm Pharm 24:119-134. ∙ Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int 40:939-947. ∙ Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864. C4 Cat. no. HYB 162 ∙ Jensen ML, Honoré C, Hummelshoj T, Hansen BE, Madsen HO, Garred P (2006) Ficolin-2 recognizes DNA and participates in the clearance of dying host cells. Mol Immunol [Epub ahead of print]. ∙ Frederiksen PD, Thiel S, Larsen CB, Jensenius JC (2005) M-fi colin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement. Scand J Immuno 62:462-73. ∙ Krarup A, Sorensen UB, Matsushita M, Jensenius JC, Thiel S (2005) Effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, L-fi colin, and H-fi colin. Infect Immun 73:1052-60. ∙ Petersen SV, Thiel S, Jensen L, Vorup-Jensen T, Koch C, Jensenius JC (2000) Control of the classical and the MBL pathway of com- plement activation. Mol Immunol 37:803-11. Factor D Cat. no. GAU 008-01 and GAU 010-04 and Factor H Cat. no. GAU 020-03 and GAU 018-03 ∙ Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm Pharm 24:119-134. ∙ Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int 40:939-947. ∙ Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative path- way of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190. MBL Cat. no. KIT 029 and HYB 131 ∙ Please view the MBL reference fl yer, for a complete list of references using KIT 029 or HYB 131. Properdin Cat. no. HYB 039 ∙ Bathum L, Hansen H, Teisner B, Koch C, Garred P, Rasmussen K, Wang P (2005) Association between combined properdin and mannose-binding lectin defi ciency and infection with Neisseria meningitidis. Mol Immunol 43:473-479. ∙ Wirthmueller U, Dewald B, Thelen M, Schafer MK, Stover C, Whaley K, North J, Eggleton P, Reid KB, Schwaeble WJ. (1997) Properdin, a positive regulator of complement activation, is released from secondary granules of stimulated peripheral blood neutrophils. J Im- munol 158:4444-51. ∙ Schwaeble W, Huemer HP, Most J, Dierich MP, Strobel M, Claus C, Reid KB, Ziegler-Heitbrock HW (1994) Expression of properdin in human monocytes. Eur J Biochem 219:759-64. For more information, please contact BioPorto Diagnostics or your local BioPorto Diagnostics distributor. Distributed by: BioPorto Diagnostics A/S Phone (+45) 4529 0000 Grusbakken 8 Fax (+45) 4529 0001 DK-2820 Gentofte E-mail [email protected] Denmark Web www.bioporto.com www.ngal.com 60001/082011/e.