Supplementary Table S2. In vitro and in vivo growth analyses. Cell In Vitro Growth (10% FBS) Soft Agar Tumors/Sites Injected Population Doubling Time Saturation Growth SC IM Density PAX-FKHR Populations MSC 41.4 16.8 0 0/4 V1 40.6 16.2 0 0/4 V2 36.4 14.6 0 0/4 P3F1 30.8 57.7 0 0/8 P3F2 32.0 54.4 0 0/8 P7F1 31.3 66.0 0 0/6 P7F2 32.2 56.2 0 0/6 + SV40-LT Populations V1-Zeo 40.2 25.8 0 0/4 V2-Zeo 41.5 25.1 0 0/6 P3F1-Zeo 26.4 74.7 0 0/4 P3F2-Zeo 31.4 84.0 0 0/4 P7F1-Zeo 27.7 75.0 0 0/4 P7F2-Zeo 29.4 34.9 0 0/4 V1-LT 18.5 176.6 0.72 ± 0.19 4/8 V2-LT 20.2 214.9 0.56 ± 0.12 0/4 P3F1-LT 22.0 261.8 0.54 ± 0.04 0/4 P3F2-LT 23.0 269.3 0.61 ± 0.06 8/8 P7F1-LT 23.7 251.3 0.63 ± 0.04 0/4 P7F2-LT 23.9 239.3 0.69 ± 0.24 0/4 + SV40-LT/H-rasG12V Populations V1-LTR 1.37 ± 0.30 3/3 V2-LTR 1.49 ± 0.16 3/3 P3F1-LTR 1.56 ± 0.11 3/3 P3F2-LTR 1.50 ± 0.08 3/3 P7F1-LTR 1.41 ± 0.01 3/3 P7F2-LTR 1.30 ± 0.14 3/3 + p53DD Populations V1-DD 0.55 ± 0.06 0/4 0/4 P3F2-DD 0.59 ± 0.01 2/4 3/3 P7F1-DD 0.52 ± 0.04 0/4 0/6 + p53DD/H-rasG12V Populations V1-DDR 1.22 ± 0.03 3/3 P3F2-DDR 1.36 ± 0.11 3/3 P7F1-DDR 1.34 ± 0.04 3/3 Doubling Time: Number of hours to undergo one population doubling, while growing exponentially. Saturation Density: Number of live cells (X 10,000) present once the culture became confluent. Soft Agar Growth: Percentage of seeded cells that formed colonies ≥ 0.1 mm in diameter. SC, subcutaneous; IM, intramuscular (gastrocnemius). Supplementary Table S3. P3F2-LT versus V1-LT up-regulated gene list. Transcript Gene Fold- Gene Name V1-LT P3F2-LT p-value Cluster ID Symbol Differenc e 6759997 Scg2 secretogranin II 5.59 11.84 76.0 1.7E-11 6972317 Igf2 insulin-like growth factor 2 6.49 12.60 69.1 1.1E-03 6978309 Slc12a3 solute carrier family 12, member 3 5.86 11.36 45.4 5.4E-11 6972405 Cdkn1c cyclin-dependent kinase inhibitor 1C (P57) 6.85 12.13 39.0 5.0E-05 6766046 Plagl1 pleiomorphic adenoma gene-like 1 7.07 12.17 34.3 3.9E-03 7018847 Itm2a integral membrane protein 2A 7.92 12.97 33.1 2.0E-06 6963271 Ppfibp2 protein tyrosine phosphatase, receptor-type, F interacting5.82 protein, binding10.47 protein 2 25.1 2.9E-08 6945113 Cpa5 carboxypeptidase A5 5.75 10.28 23.2 8.4E-04 6819250 1110028A07Rik RIKEN cDNA 1110028A07 gene 5.84 10.31 22.2 1.6E-06 6753268 Myog myogenin 6.55 10.99 21.7 6.5E-06 6954557 Smyd1 SET and MYND domain containing 1 6.28 10.66 20.9 1.9E-04 6885516 1110002H13Rik RIKEN cDNA 1110002H13 gene 6.23 10.56 20.1 4.8E-05 6802727 Dio2 deiodinase, iodothyronine, type II 6.96 11.29 20.1 2.0E-07 6900052 Casq2 calsequestrin 2 6.20 10.44 18.9 7.4E-03 7019970 Dcx doublecortin 5.59 9.82 18.8 1.3E-03 6960454 Myod1 myogenic differentiation 1 5.96 10.18 18.7 9.9E-10 6912565 Cnr1 cannabinoid receptor 1 (brain) 6.09 10.25 17.9 7.7E-10 6972750 --- --- 6.32 10.45 17.5 1.3E-08 6751326 Chrnd cholinergic receptor, nicotinic, delta polypeptide 6.39 10.48 17.1 1.1E-08 6892955 Tnnc2 troponin C2, fast 6.85 10.87 16.3 5.3E-04 6778044 Erbb3 v-erb-b2 erythroblastic leukemia viral oncogene homolog6.10 3 (avian) 10.08 15.8 2.9E-07 6751330 Chrng cholinergic receptor, nicotinic, gamma polypeptide 6.26 10.24 15.7 1.6E-05 6771569 Stac3 SH3 and cysteine rich domain 3 6.64 10.49 14.4 1.3E-03 6789908 Pipox pipecolic acid oxidase 5.91 9.75 14.4 2.9E-11 6972314 H19 H19 fetal liver mRNA 8.53 12.37 14.3 5.2E-06 6795033 Coch coagulation factor C homolog (Limulus polyphemus) 5.46 9.22 13.5 1.0E-02 6867859 Pygm muscle glycogen phosphorylase 7.08 10.78 13.0 3.0E-03 6801807 Kcnh5 potassium voltage-gated channel, subfamily H (eag-related),5.92 member 59.60 12.8 2.0E-08 6964285 Mylpf myosin light chain, phosphorylatable, fast skeletal muscle7.39 10.99 12.1 1.9E-03 6787743 Cyfip2 cytoplasmic FMR1 interacting protein 2 6.81 10.39 11.9 6.0E-10 6843355 Sim2 single-minded homolog 2 (Drosophila) 6.26 9.81 11.7 4.9E-03 6998603 Dock3 dedicator of cyto-kinesis 3 6.27 9.81 11.7 7.9E-07 6990663 Klhl31 kelch-like 31 (Drosophila) 5.89 9.43 11.6 7.9E-03 6913195 Tmod1 tropomodulin 1 5.98 9.47 11.3 1.0E-05 6753397 Tnni1 troponin I, skeletal, slow 1 6.70 10.19 11.2 9.9E-05 6843642 Srl sarcalumenin 6.48 9.96 11.2 2.2E-05 6999522 Cck cholecystokinin 5.76 9.21 11.0 6.3E-03 6959000 Rtn2 reticulon 2 (Z-band associated protein), transcript variant7.47 C 10.91 10.9 2.4E-05 6834108 Cdh6 cadherin 6 6.03 9.47 10.9 1.0E-05 6807214 Fgfr4 fibroblast growth factor receptor 4 6.21 9.63 10.7 1.0E-06 6925654 Serinc2 serine incorporator 2 7.11 10.44 10.1 3.3E-07 7018819 Zcchc5 zinc finger, CCHC domain containing 5 5.48 8.81 10.0 2.7E-03 6800314 Lrrn3 leucine rich repeat protein 3, neuronal 6.73 10.05 10.0 2.4E-08 6776156 Plxnc1 plexin C1 6.80 10.11 9.9 9.0E-08 6757035 Eya1 eyes absent 1 homolog (Drosophila) 5.76 9.03 9.7 4.3E-05 6868845 Dmrt2 doublesex and mab-3 related transcription factor 2 5.89 9.14 9.5 3.7E-03 6759664 Igfbp5 insulin-like growth factor binding protein 5 9.22 12.45 9.4 1.2E-06 6953144 --- --- 6.74 9.90 9.0 1.4E-07 6979655 Cdh15 cadherin 15 6.33 9.47 8.9 5.3E-07 6763295 Ralgps2 Ral GEF with PH domain and SH3 binding motif 2 7.04 10.16 8.7 6.2E-05 6908748 Tmem56 transmembrane protein 56 5.50 8.60 8.6 4.0E-04 6879015 Rapsn receptor-associated protein of the synapse 6.11 9.21 8.6 5.9E-06 6756536 Plxna2 plexin A2 7.21 10.29 8.5 1.7E-05 6766491 Slc2a12 solute carrier family 2 (facilitated glucose transporter), member5.82 12 8.90 8.5 5.9E-04 6771092 Grip1 glutamate receptor interacting protein 1 5.94 9.01 8.4 9.5E-05 6955514 Nup210 nucleoporin 210 6.15 9.22 8.4 3.9E-04 6966198 Spint2 serine protease inhibitor, Kunitz type 2 6.02 9.09 8.4 2.4E-05 6966183 Ryr1 ryanodine receptor 1, skeletal muscle 6.13 9.13 8.0 2.1E-04 6952436 Tsga14 testis specific gene A14 7.26 10.24 7.9 2.3E-06 7012265 Tmem47 transmembrane protein 47 7.27 10.25 7.9 3.5E-05 6848947 Lix1 limb expression 1 homolog (chicken) 5.73 8.69 7.8 3.6E-04 6861173 Megf10 multiple EGF-like-domains 10 6.21 9.15 7.7 3.9E-07 6942503 Emid2 EMI domain containing 2 6.23 9.13 7.5 2.0E-03 6801902 Spnb1 spectrin beta 1 6.03 8.92 7.4 4.5E-04 6967269 Dbx1 developing brain homeobox 1 5.97 8.79 7.0 8.4E-06 6962491 Sytl2 synaptotagmin-like 2 7.01 9.81 7.0 9.7E-06 6760251 4930544G21Rik RIKEN cDNA 4930544G21 gene 5.44 8.25 7.0 2.8E-03 6758027 Tbc1d8 TBC1 domain family, member 8 6.39 9.17 6.8 4.9E-07 6998643 Slc38a3 solute carrier family 38, member 3 5.68 8.44 6.7 2.8E-04 6777956 Nxph4 neurexophilin 4 6.55 9.30 6.7 5.4E-03 6912517 Rragd Ras-related GTP binding D 6.13 8.85 6.6 1.7E-06 6979318 AI427515 expressed sequence AI427515 6.04 8.76 6.6 1.1E-04 6803918 Nudt14 nudix (nucleoside diphosphate linked moiety X)-type motif7.33 14 10.05 6.6 2.9E-05 6789343 Chrnb1 cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)7.78 10.49 6.6 1.3E-05 6945111 Cpa4 carboxypeptidase A4 5.78 8.50 6.6 5.9E-03 6817645 LOC100039227 similar to RIKEN cDNA 1110051B16 gene 5.78 8.47 6.5 1.0E-06 6965264 Tnni2 troponin I, skeletal, fast 2 6.98 9.65 6.3 2.6E-03 7014085 Nrk Nik related kinase 6.64 9.29 6.3 2.5E-06 6798313 Crip2 cysteine rich protein 2 8.07 10.72 6.3 7.6E-06 6882700 Ctnnbl1 catenin, beta like 1 8.29 10.91 6.2 6.2E-07 6992946 Entpd3 ectonucleoside triphosphate diphosphohydrolase 3 5.74 8.31 6.0 4.7E-05 6947985 Hdac11 histone deacetylase 11 6.53 9.07 5.8 4.4E-03 6837090 --- --- 6.05 8.57 5.7 4.5E-05 6786045 Ddc dopa decarboxylase 5.77 8.26 5.6 3.9E-06 6952070 Tspan12 tetraspanin 12 8.02 10.52 5.6 3.7E-03 6948274 Kbtbd8 kelch repeat and BTB (POZ) domain containing 8 6.64 9.14 5.6 1.2E-06 6781492 Kcnj12 potassium inwardly-rectifying channel, subfamily J, member5.63 12 8.10 5.5 3.6E-07 6822485 --- --- 6.32 8.78 5.5 1.7E-06 6958984 Dmpk dystrophia myotonica-protein kinase 7.86 10.30 5.4 2.2E-07 6769448 Rfx4 regulatory factor X, 4 (influences HLA class II expression)5.88 8.32 5.4 3.9E-04 6870622 Adrb1 adrenergic receptor, beta 1 6.17 8.60 5.4 4.6E-03 6917277 Fndc5 fibronectin type III domain containing 5 5.94 8.37 5.4 8.4E-03 6762353 Adora1 adenosine A1 receptor 6.42 8.82 5.3 8.7E-04 6760292 Dner delta/notch-like EGF-related receptor 6.05 8.42 5.2 2.7E-04 6772906 Lama2 laminin, alpha 2 6.62 8.97 5.1 1.1E-05 6750625 Des desmin 7.94 10.28 5.1 2.2E-05 6883186 Eya2 eyes absent 2 homolog (Drosophila) 6.48 8.79 5.0 7.6E-04 6949850 --- --- 7.82 10.12 4.9 5.9E-04 6845943 Boc biregional cell adhesion molecule-related/down-regulated6.90 by oncogenes9.19 (Cdon) binding4.9 protein 3.9E-05 6840841 Popdc2 popeye domain containing 2 5.59 7.87 4.9 2.4E-03 6817978 Tnnc1 troponin C, cardiac/slow skeletal 7.15 9.43 4.9 9.4E-03 6806791 Cap2 CAP, adenylate cyclase-associated protein, 2 (yeast) 6.66 8.95 4.9 6.6E-04 6799054 Mycn v-myc myelocytomatosis viral related oncogene, neuroblastoma6.30 derived8.58 (avian) 4.9 2.4E-03 6825410 Fzd3 frizzled homolog 3 (Drosophila) 7.54 9.82 4.9 4.1E-06 6965233 Brsk2 BR serine/threonine kinase 2 5.65 7.92 4.8 2.7E-03 6852031 Myom1 myomesin 1 6.10 8.38 4.8 2.9E-03 6932409 --- --- 6.29 8.56 4.8 3.4E-04 7012860 Foxo4 forkhead box O4 7.34 9.57 4.7 3.8E-05 6757157 Jph1 junctophilin 1 5.87 8.11 4.7 5.1E-04 6791000 Sgca sarcoglycan, alpha (dystrophin-associated glycoprotein)5.99 8.22 4.7 5.2E-04 6953762 Scrn1 secernin 1 7.71 9.93 4.7 2.5E-05 6960390 --- --- 7.33 9.56 4.7 1.1E-06 6812215 Tubb2b tubulin, beta 2b 7.36 9.58 4.7 2.9E-04 6899254 Kcnn3 potassium intermediate/small conductance calcium-activated6.48 channel, 8.68subfamily N, member4.6 3 7.8E-03 6979675 Cpne7 copine VII 5.98 8.18 4.6 3.4E-04 6819442 Fgf9 fibroblast growth factor 9 6.69 8.88 4.6 5.6E-04 6912371 Epha7 Eph receptor A7 7.20 9.38 4.5 2.7E-05 6949884 Vwf Von Willebrand factor homolog 6.26 8.42 4.5 1.1E-04