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Journaf of Threatened Taxa Buffdfng evfdence for conservafon gfobaffy www.threatenedtaxa.org ISSN 0974-7907 (Onffne) | ISSN 0974-7893 (Prfnt) Short Communfcatfon New records of the subfamffy Stratfomyfnae (Dfptera: Stratfomyfdae) from Pakfstan Muhammad Asghar Hassan, Imran Bodfah, Zafar Iqbaf & Rubfna Jabeen 26 October 2017 | Vof. 9| No. 10 | Pp. 10831–10834 10.11609/jot. 3562 .9. 10. 10831-10834 For Focus, Scope, Afms, Poffcfes and Gufdeffnes vfsft htp://threatenedtaxa.org/About_JoTT For Arfcfe Submfssfon Gufdeffnes vfsft htp://threatenedtaxa.org/Submfssfon_Gufdeffnes For Poffcfes agafnst Scfenffc Mfsconduct vfsft htp://threatenedtaxa.org/JoTT_Poffcy_agafnst_Scfenffc_Mfsconduct For reprfnts contact <[email protected]> Pubffsher/Host Partner Threatened Taxa Journal of Threatened Taxa | www.threatenedtaxa.org | 26 October 2017 | 9(10): 10831-10834 New records of the subfamily Stratiomyinae (Diptera: Stratiomyidae) from Pakistan 1 2 3 4 ISSN 0974-7907 (Online) Muhammad Asghar Hassan , Imran Bodlah , Zafar Iqbal & Rubina Jabeen Communication Short ISSN 0974-7893 (Print) 1,2,3 Department of Entomology, Pir Mehr Ali Shah Arid Agricultural University, Rawalpindi, Pakistan 4 Department of Biochemistry, Faculty of Biological Sciences, Quaid-e-Azam University, Islamabad, Pakistan OPEN ACCESS 1 [email protected] (corresponding author), 2 [email protected], 3 [email protected], 4 [email protected] Abstract: Based on collected specimens from the Narowal, Rawalpindi of different genera. Scutellum is often adorned with and Jhelum districts of (Punjab Province) Pakistan, three species were spines and this may be the reason for the common name recognised in two genera, under the subfamily Stratiomyinae (Diptera: Stratiomyidae). Odontomyia ochropa Thomson, 1869, Oplodontha ‘soldier flies’. Their body size varies from 2–20 mm with minuta (Fabricius, 1794) and Oplodontha rubrithorax (Macquart, different colourations, some are wasp mimics, marked 1838) are recorded for the first time from Pakistan. Photographs, diagnostic characters, descriptions, geographical distributions and with different colourations such as black and yellow or identification keys of the described species are provided. green and sometimes metallic (Woodley 2001; Kovac & Rozkošný 2004). Keywords: Diptera, new records, Pakistan, Stratiomyidae, Stratiomyinae. James (1975) provided a detailed list of 79 species of family Stratiomyidae from India in the Oriental Catalogue of Diptera. Later, Woodley (2001) published the world catalogue of soldier flies. Wang et al. (2007) described Soldier flies (Stratiomyidae) belong to the suborder a new species of genus Odontomyia from Taiwan. Ting Brachycera, infraorder Stratiomyomorpha and are et al. (2009) worked on the genus Oplodontha with the the sister group of the Xylomyidae (James 1981). description of three new species, and also constructed Stratiomyidae encompasses 2,690 described species, the local key to the known species of the genus from 385 genera and 12 subfamilies, which are distributed China. Kazerani & Khaghaninia (2013) reported the genus worldwide (Pape et al. 2011 ). The adult soldier flies Oplodontha for the first time from Iran. Nerudová et al. usually prefer to live near the larval habitats mostly found (2015) gave the first detailed description of both larvae and in wetlands, damp places, under bark, on animal excreta puparium of Oplodontha rubrithorax (Macquart, 1838) and decaying organic matter. The larvae of many species from the Oriental Region. Roy et al. (2016) reported nine live in aquatic environments, in dung and decaying organic from the Sunderban Biosphere Reserve of India. Besides matter and under bark; a few are dependent on grasses establishing a checklist of 84 species under 35 genera in (James 1968; Sheppard 1983; Samson 2001; Woodley seven subfamilies of Indian stratiomyid fauna, Wachkoo 2001). This family has important diagnostic characters of et al. (2017) also reported five species belonging to strong anterior veins on their wings and a small discal cell two subfamilies namely Clitellariinae and Sarginae from in the anterior half of the wings. The antennae have many Pakistan. So the current study was planned to explore the structural variations and are used for the identification fauna of subfamily Stratiomyinae from Pakistan. DOI: http://doi.org/10.11609/jott.3562.9.10.10831-10834 | ZooBank: urn:lsid:zoobank.org:pub:05E8859F-55DA-4BFC-B508-CCF15A04C669 Editor: M. Mike Kerry, Seaford, East Sussex, UK. Date of publication: 26 October 2017 (online & print) Manuscript details: Ms # 3562 | Received 11 June 2017 | Final received 05 October 2017 | Finally accepted 10 October 2017 Citation: Hassan, M.A., I. Bodlah, Z. Iqbal & R. Jabeen (2017). New records of the subfamily Stratiomyinae (Diptera: Stratiomyidae) from Pakistan. Journal of Threatened Taxa 9(10): 10831–10834; http://doi.org/10.11609/jott.3562.9.10.10831-10834 Copyright: © Hassan et al. 2017. Creative Commons Attribution 4.0 International License. JoTT allows unrestricted use of this article in any medium, reproduction and distribution by providing adequate credit to the authors and the source of publication. Funding: None. Competing interests:The authors declare no competing interests. 10831 New records of Stratiomyinae from Pakistan Hassan et al. Materials and Methods Genus Odontomyia Meigen, 1803 The studied specimens were collected during the Diagnostic Characters: Male eyes holoptic while female months of May, June and July (2016–17) from different dichoptic, eyes bare; antennae with six flagellomeres, localities of the Narowal, Rawalpindi and Jhelum districts ratio of scape to pedicel usually not more than 1.5: 1, of (Punjab Province) Pakistan. The adult specimens were last two flagellomeres forming a short but distinct stylus; collected using a sweep net from grasses, a maize field, scutellum with a pair of distinct spines; wings with cross- wild flowers and flowers of Parthenium hysterophorus. vein m-cu present; branches of M often weakened (Dutta The collected specimens were killed by placing them in et al. 1997; Wang et al. 2007; Roy et al. 2016). a killing jar having Potassium cyanide for 20–30 minutes. Later, the pinned specimens were identified under an Odontomyia ochropa Thomson, 1869 Olympus SZX7, Model SZ2-ILST light stereoscope and Image 1 A–C photographs were prepared under a Nikon SMZ 1500 Material Examined: Registration number: 100. Punjab Binocular stereoscope, with a Nikon Digital Sight DS-Fi1 (Pakistan), Shakargarh, 282m, 13 males, 13.vii.2016, leg. camera attached. The following literature were used: M.A. Hassan. Brunetti (1920), Dutta et al. (1997), Wang et al. (2007), Distribution: India, Pakistan (new record), Philippines, Tkoč & Rozkošný 2014 and Roy et al. (2016) for the Singapore, Thailand, USA (Wachkoo et al. 2017). identification of the species. The identified specimens Description: Male: Head blackish (Image 1A); eyes were deposited at the National Insect Museum, bare, contiguous in male, upper facets large; vertical Islamabad, Pakistan. triangle black, ocelli brownish, frontal triangle and face orange, face with distinct bare tubercle, face below with Results and Discussion greyish hairs; proboscis black, papillae yellow; antennae During the current study three species—Odontomyia orange, flagellomeres slightly brownish, four annulations ochropa Thomson, 1869, Oplodontha minuta (Fabricius, with narrow long pointed style; thorax black, scutum with 1794), and Oplodontha rubrithorax (Macquart, 1838)— short close golden yellow pubescence, which, except were recorded for the first time from Pakistan. Wachkoo along the lateral sides, having longer hairs, scutum with et al. (2017) reported five species belonging to two distinct transverse suture separated from the centre, post- subfamilies namely Clitellariinae and Sarginae, viz., pronotum and postalar callus yellow, scutellum yellow Adoxomyia heminopla (Wiedemann, 1819), Microchrysa with basal blackish spot in the centre, apical spines yellow flaviventris (Wiedemann, 1824), Ptecticus melanurus slightly pointed at tip, fringe of yellow hairs along the hind (Walker, 1848), Sargus gemmifer Walker, 1849, and Sargus margin of scutellum; pleuron mostly yellow with yellow mactans Walker, 1859 from Pakistan without mentioning hairs, sternopleuron on lower side black, area between the exact locality in Pakistan. fore and mid legs broadly black with distinct longitudinal suture, yellowish pubescence; legs orange, tip of tarsi Subfamily Stratiomyinae slightly brownish; halteres yellow, basally black, discal cell Diagnostic Characters: Abdomen with five visible of normal size, wings clear, basal veins yellow (Image 1C); abdominal tergites; scutellum with two straight and abdomen pale yellow, first tergite with elongated black moderately long or short spines; cell dm present; four spot in the middle, second, third and fourth each with a veins arising from cell dm; antennal flagellum comprising large black spot in the middle reaching from anterior to six flagellomeres,