Rec. zool. Surv. India: Vol. 117(4)/ 383-390, 2017 ISSN (Online) : (Applied for) DOI: 10.26515/rzsi/v117/i4/2017/121292 ISSN (Print) : 0375-1511 Significance of the chaetotaxy in larval identification of Pyrausta bambucivora Moore (Lepidoptera: Crambidae: Pyraustinae) D. S. Deep1*, P. C. Pathania2 and H. S. Rose3 1Department of Life Sciences, RIMT University, Mandi Gobindgarh – 147301, Punjab, India, [email protected] 2Zoological Survey of India, M-Block, New Alipore – 700053, Kolkata, India 3Department of Zoology and Environmantal Sciences, Punjabi University, Patiala – 147002, Punjab, India Abstract The identification and diagnosis of insect pest can bestPyrausta be strengthen bambucivora through an examination of the arrangement of various setae and punctures of different body segments. In the current study, the cephalic region and other body segments (thoracic, abdominal) of the last instar caterpillar of Moore (Crambidae) have been examined thoroughlyKeywords and: contingent the occurrence of 17 tactile setae, 4 proprioceptors and 10 pores on each half of the head. Chaetotaxy, Crambidae, Lepidoptera, Pyraustinae Introduction Material and Methods Pyrausta bambucivora Moore is commonly known as Immature stages of Pyrausta bambucivora Moore has been bamboo leaf roller. Larvae of this species rigorously attack collected from Punjab and reared in the laboratory up to bamboo tree and left it deskeletonized after feeding on pupal stage on fresh host plant leaves. To study chaetotaxy them. The identification and control of this pest species in legitimate manner, the complete distention of the larval is much more important at imago stage than that at adult body was done by boiling the mature larvae in the test by studying their biology and chaetotaxy. The purpose tube. For proper maceration isolated heads and body were to carry out chaetotaxic studies of aforesaid species is to kept in KOH (10%) for 7-9 hrs followed by washing in 1% identify the different setae and puncture on the larval head glacial acetic acid followed by mounting in 70% alcohol and other body segments by studying their arrangements, and glycerine in 9:1 ratio. For setal study, isolated heads distribution pattern and size, which further help to of caterpillar shifted to petridish containing glycerine classify the species. Globally, the minority of workers whereas permanent slides of thorax and abdomen were such as Mathur (1954 and 1959), Singh (1956), Mathur made by fixing them in DPX on glass slide and then and Singh (1963), Azam and Ali (1965), Franzmann observed under stereoscopic zoom microscope. and Garrett (1978), Yoshiyasu (1980), Goel and Kumar (1981), Stehr (1987), Lin (1993), Amutha and David Results (1998), Rose and Singh (2010) and Pinheiro et. al., (2011) Head: Cranium moderately sclerotized, golden brown have given significant contribution in intensification of in colour and somewhat shiny; median epicranial suture the chaetotaxic study. So due to paucity of chaetotaxic less than half the length of adfrontal suture; front clypeus information and nomenclature, in the present paper, we longer than broad; ecdysial line wavy joining in middle endow with detailed descriptions of the chaetotaxic study to medium epicranial suture; mouth parts strongly of Pyrausta bambucivora Moore along with diagram’s. * Author for correspondence Article Received on: 10.11.2017 Accepted on: 19.12.2017 Significance of the chaetotaxy in larval identification of Pyrausta bambucivora Moore (Lepidoptera: Crambidae: Pyraustinae) sclerotized; stemmatal area somewhat differentiated from MDa lies close and posterior to MD2 (Plate-1, Figure 1 rest of cranium, having six stemmata, fifth stemmata & 2). present near the base of antenna and sixth behind fifth stemmata; stemmata 1, 2 and 6 large and almost equal in Thoracic Chaetotaxy: Spine like tactile setae. size; stemmata 3 present away from stemmata 2; each half T1 (Plate-1, fig.3): Prothoracic shield dark brown strongly of head represents 17 tactile setae, 4 proprioceptors and sclerotized, elongated and bent inwards; anterior margin 10 pores; all setae spine like arise from pinacula on the straighter and longer, posterior margin short and notched last larval instar. in the middle with edges rounded; each half with six setae Frontal group seta F1 and pore Fa; F1 situated much and two pores; XD group present near anterior margin closer to lateral margin of frons, directly postrad to C2, of shield; XD1 lies above and anterad to XD2; pore XDa whereas, pore Fa lies near median longitudinal line; present posterodorsad to XD1; XDb anterodorsad to XD2; present dorsad to F1. Clypeal group contains setae C1 XDa and XD1 situated close to each other. Dorsal group and C2, the latter present close to epicondyle, C1 slightly present near posterior margin of shield; D1 anterodorsad shorter than C2 and present towards median longitudinal to D2; D1 close to middorsal line; D2 longer than D1 in line. Setae AF1 and AF2 along with pore AFa constitutes length. Subdorsal group situated on posterolateral margin adfrontal group; AF2 slightly more than half the length of of shield; SD1 longer than SD2; SD1 anterolatrad to SD2. AF1, situated posterad to point where median epicranial Lateral group bisetose, with setae L1 and L2 sharing same suture joins the lateral adfrontal suture near median pinaculum; the latter prespiracle in position; L1 longer epicranial suture, AF1 posteromesad to A1, close to than L2; L2 directly laterad to SD1; L1 posteroventrad to lateral frontal suture; pore AFa lies near the mid line and L2. Subventral group lies above leg base, bisetose, beset also anterad to AF2. Setae A1, A2, A3 and pore Aa located on common oblong pinaculum; SV2 shorter and anterior on the anterodorsal area, represent anterior group; A1 in to SV1. Ventral group consisted of single tactile seta V1, level of stemmata 3, lies towards median longitudinal present below coxa near midventral line. Microscopic line; A2 present almost in straight line posterior to A1; setae bear two groups MXD and MV; MXD1 equally A3 posterolatrad to A2; A3>A1>A2 lengthwise; pore Aa distant from D1 and D2 and lies close to posterior lies close to A2 than A3 and posteromesad to A2. Setae margin of thoracic shield; MV group represented by setae P1 and P2 along with pore Pb denotes as posteriodorsal MV2 and MV3; MV3 lying precoxal in position; MV2 group; P1 greatly longer than P2; P2 posterolaterad of posterodorsad to MV3. P1; pore Pb closer and anterad to P2. Seta L1 represents lateral group; pore La absent; L1 posterolatrad to A3. T2 and T3 (Plate-1, fig. 4): Dorsal group composed of two Stemmatal area inundated with setae S1, S2, and S3 in setae D1 and D2 share same pinaculum; D1 posterodorsad addition to pore Sa and Sb; S1 present inside semicircle to D2; D2 longer than D1. Subdorsal group comprises of stemmata, near but ventrocaudad to second stemmata; setae SD1 and SD2 present on common pinaculum; SD1 S2 caudad to stemmata 1, S3 present posterad to sixth anterolaterad to SD2. Lateral group trisetose with setae stemmata ; S2>S1>S3 lengthwise; pore Sa lies dorsad to L1 and L2 present on common pinaculum and L3 on stemmata 6; pore Sb lies very close and behind stemmata separate irregular pinaculum; L1 anteroventrad to SD1; L2 3. Substemmatal area contains setae SS1, SS2 and SS3; SS1 anteroventrad to L1; L3 posterodorsad to L2. Subventral lies near and below stemmata 6, SS2 lies close and caudad group unisetose; seta SV1 lying on subtraingular to stemmata 6; SS3 dorsocaudad to SS1; SS1>SS3<SS2 pinaculum, present anteroventrad to L3. Seta V1 situated lengthwise; pore SSa absent. Seta MG1 and pore MGa posteroventrad to base of leg close to midventral line. constitutes genal group; MG1 present at lower and MD1 situated close to the anterior margin of segment and rear portion of the head; pore MGa lies dorsoanterad anterolaterad to D2; proprioceptors MSD1 and MSD2 to seta MG1. Microdorsal group furnished with setae lying anterad to seta SD1; MSD1 posterodorsad to MSD2; MD1, MD2, MD3 and pore MDa; MD1 dorsolaterad to microsetae MV1, MV2 and MV3 situated opposite leg; P2; MD2 lies in middle of MD1 and MD3 and present MV3 anteroventrad to MV2 and latter posteroventrad to posteromesad to MDa; MD3 posterodorsad to MD2; pore MV1. 384 Vol 117(4) | 2017 | www.recordsofzsi.com Zoological Survey of India D. S. Deep, P. C. Pathania and H. S. Rose Abdominal Chaetotaxy A9 (Plate-1, fig. 9): Dorsal group bisetose having setae D1 and D2; D1 seta share same pinaculum with subventral A1, A2, A7 and A8 (Plate-1, fig.-5,7): Setae of dorsal group group; D1 anteroventrad to D2; D1 shorter than D2. D1 and D2 present close to middorsal line of segment; in Subdorsal group unisetose, seta SD1 posteroventrad A1 and A7, D1 longer and anterodorsad to D2; but in A2 to D1, SD1 thin and hair-like, much shorter than D1. and A8, D2 somewhat longer than D1. Subdorsal group Lateral group unisetose with seta L1 posteroventrad to possesses setae SD1 and SD2; SD1 longer than SD2 and SD1. Subventral seta SV1 posteroventrad to L1. Seta V1 encircled by rounded pinaculum; SD2 microscopic; in all posteroventrad to SV1 and lies close to ventral meson. the segments, SD1 dorsad to spiracle; SD2 anterodorsad Microsetae MD1 and MV3 lies near anterior margin of to spiracle. Lateral group trisetose, represented by setae segment; MD1 anteroventrad to D2; MV3 anteroventrad L1, L2 and L3; L1 and L2 present on common pinaculum; to SV1. L3 present on a separate irregular pinaculum; L1 posteroventrad to spiracle; L2 anteroventrad to L1; L3 A10(Plate-1, fig.-10): Anal shield well developed; anterior posteroventrad to L1; L3>L2>L1 lengthwise. Subventral margin straight; posterior margin more or less rounded; group contains setae SV1, SV2 and SV3; in segment A2, D1 present near anterior margin of shield; D1 longer than subventral group trisetose, beset with setae SV1, SV2 D2; D2 lies at distal margin of shield.