Supplementary Table 2. Differentially Expressed Genes following Sham treatment relative to Untreated Controls Fold Change Accession Name Symbol 3 h 12 h NM_013121 CD28 antigen Cd28 12.82 BG665360 FMS-like tyrosine kinase 1 Flt1 9.63 NM_012701 Adrenergic receptor, beta 1 Adrb1 8.24 0.46 U20796 Nuclear receptor subfamily 1, group D, member 2 Nr1d2 7.22 NM_017116 Calpain 2 Capn2 6.41 BE097282 Guanine nucleotide binding protein, alpha 12 Gna12 6.21 NM_053328 Basic helix-loop-helix domain containing, class B2 Bhlhb2 5.79 NM_053831 Guanylate cyclase 2f Gucy2f 5.71 AW251703 Tumor necrosis factor receptor superfamily, member 12a Tnfrsf12a 5.57 NM_021691 Twist homolog 2 (Drosophila) Twist2 5.42 NM_133550 Fc receptor, IgE, low affinity II, alpha polypeptide Fcer2a 4.93 NM_031120 Signal sequence receptor, gamma Ssr3 4.84 NM_053544 Secreted frizzled-related protein 4 Sfrp4 4.73 NM_053910 Pleckstrin homology, Sec7 and coiled/coil domains 1 Pscd1 4.69 BE113233 Suppressor of cytokine signaling 2 Socs2 4.68 NM_053949 Potassium voltage-gated channel, subfamily H (eag- Kcnh2 4.60 related), member 2 NM_017305 Glutamate cysteine ligase, modifier subunit Gclm 4.59 NM_017309 Protein phospatase 3, regulatory subunit B, alpha Ppp3r1 4.54 isoform,type 1 NM_012765 5-hydroxytryptamine (serotonin) receptor 2C Htr2c 4.46 NM_017218 V-erb-b2 erythroblastic leukemia viral oncogene homolog Erbb3 4.42 3 (avian) AW918369 Zinc finger protein 191 Zfp191 4.38 NM_031034 Guanine nucleotide binding protein, alpha 12 Gna12 4.38 NM_017020 Interleukin 6 receptor Il6r 4.37 AJ002942 Retinoic acid receptor, beta Rarb 4.35 X70706 Plastin 3 (T-isoform) Pls3 4.13 AJ000556 Janus kinase 1 Jak1 4.08 NM_053679 DNA fragmentation factor, alpha subunit Dffa 4.08 NM_057118 Contactin 1 Cntn1 4.07 NM_017247 Sodium channel, voltage-gated, type 10, alpha Scn10a 3.96 polypeptide NM_031563 Nuclease sensitive element binding protein 1 Nsep1 3.84 AF038388 FYVE, RhoGEF and PH domain containing 4 Fgd4 3.83 Y00697 Cathepsin L Ctsl 3.81 NM_031615 Zinc finger protein 148 Znf148 3.69 NM_019306 FMS-like tyrosine kinase 1 Flt1 3.67 NM_019378 SNAP25-interacting protein Snip 3.62 NM_031535 Bcl2-like 1 Bcl2l1 3.59 1 Downloaded from iovs.arvojournals.org on 09/30/2021 M92340 Interleukin 6 signal transducer Il6st 3.57 NM_017031 Phosphodiesterase 4B Pde4b 3.55 NM_012866 Nuclear transcription factor-Y gamma Nfyc 3.45 NM_013226 Ribosomal protein L32 Rpl32 3.43 NM_031018 Activating transcription factor 2 Atf2 3.37 NM_013108 Adrenergic receptor, beta 3 Adrb3 3.34 NM_012863 Muscle, intestine and stomach expression 1 Mist1 3.29 M13979 Solute carrier family 2 (facilitated glucose transporter), Slc2a1 3.28 member 1 NM_130739 Acyl-CoA synthetase long-chain family member 6 Acsl6 3.27 NM_019376 Tyrosine 3-monooxgenase/tryptophan 5-monooxgenase Ywhag 3.27 activation protein, gamma polypeptide NM_019180 Mast cell protease 6 Mcpt6 3.25 NM_012893 Actin, gamma 2 Actg2 3.18 NM_133597 Cholinergic receptor, nicotinic, beta polypeptide 3 Chrnb3 3.17 NM_012735 Hexokinase 2 Hk2 3.16 0.33 AA892567 Archain Arcn1 3.12 BE107464 ARP2 actin-related protein 2 homolog (yeast) (predicted) Actr2 3.12 X65747 Guanine nucleotide binding protein, alpha transducing 3 Gnat3 3.10 0.46 NM_012963 High mobility group box 1 Hmgb1 3.08 NM_017344 Glycogen synthase kinase 3 alpha Gsk3a 3.08 NM_053459 RAB27B, member RAS oncogene family Rab27b 3.05 NM_012610 Nerve growth factor receptor (TNFR superfamily, Ngfr 3.05 member 16) NM_053868 Neuroligin 1 Nlgn1 3.00 AI045600 Translocating chain-associating membrane protein Tram1 2.98 AF378093 Sodium channel, voltage-gated, type III, beta Scn3b 2.97 NM_021855 Cone-rod homeobox protein Crx 2.97 NM_013189 Guanine nucleotide binding protein, alpha z subunit Gnaz 2.96 NM_019343 Regulator of G-protein signaling 7 Rgs7 2.95 U77697 Platelet/endothelial cell adhesion molecule Pecam 2.95 NM_130822 Calcium-independent alpha-latrotoxin receptor homolog Lphn3 2.95 3 NM_057115 Protein tyrosine phosphatase, non-receptor type 12 Ptpn12 2.94 NM_013177 Glutamate oxaloacetate transaminase 2 Got2 2.93 NM_017041 Protein phosphatase 3, catalytic subunit, alpha isoform Ppp3ca 2.91 NM_019204 Beta-site APP cleaving enzyme Bace 2.90 NM_016990 Adducin 1 (alpha) Add1 2.88 U70050 Jagged 2 Jag2 2.84 BF555949 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Hmgcr 2.84 NM_017140 Dopamine receptor D3 Drd3 2.81 NM_013155 Very low density lipoprotein receptor Vldlr 2.80 AW919190 Tropomyosin 1, alpha Tpm1 2.77 NM_017049 Solute carrier family 4, member 3 Slc4a3 2.77 U54632 Ubiquitin-conjugating enzyme E2I Ube2i 2.76 AF031879 Neurofilament, heavy polypeptide Nefh 2.76 2 Downloaded from iovs.arvojournals.org on 09/30/2021 AF087696 Membrane protein, palmitoylated 2 (MAGUK p55 Mpp2 2.76 subfamily member 2) AF030050 Replication factor C 1 Recc1 2.75 AF083329 Interleukin 12 receptor, beta 2 Il12rb2 2.75 AI599641 Bromodomain-containing 2 Brd2 2.75 NM_012504 ATPase, Na+/K+ transporting, alpha 1 polypeptide Atp1a1 2.74 NM_019283 Solute carrier family 3 (activators of dibasic and neutral Slc3a2 2.74 amino acid transport), member 2 NM_017198 P21 (CDKN1A)-activated kinase 1 Pak1 2.72 NM_053818 Solute carrier family 6 (neurotransmitter transporter, Slc6a9 2.71 glycine), member 9 NM_053357 Catenin beta Catnb 2.70 NM_019326 Neurogenic differentiation 2 Neurod2 2.70 0.45 NM_022856 Ngfi-A binding protein 1 Nab1 2.68 M59786 Calcium channel, voltage-dependent, L type, alpha 1C Cacna1c 2.68 subunit AA892364 WW domain binding protein 11 (predicted) Wbp11 2.67 NM_019235 Gamma-glutamyltransferase-like activity 1 Ggtla1 2.63 NM_019315 Potassium intermediate/small conductance calcium- Kcnn3 2.63 activated channel, subfamily N, member 3 X64403 CCAAT/enhancer binding protein (C/EBP), gamma Cebpg 2.62 BE109664 Casein kinase II, alpha 1 polypeptide Csnk2a1 2.60 NM_019358 Glycoprotein 38 Gp38 2.60 X76996 Granzyme B Gzmb 2.60 X69523 Retinol binding protein 3 Rbp3 2.59 NM_053554 Phosphatidylinositol binding clathrin assembly protein Picalm 2.59 BF281741 Glioma tumor suppressor candidate region gene 2 LOC2926 2.58 24 NM_030843 Syntaxin binding protein 5 (tomosyn) Stxbp5 2.58 NM_017303 Potassium voltage-gated channel, shaker-related Kcnab1 2.57 subfamily, beta member 1 M90661 Insulin receptor-related receptor Insrr 2.57 NM_021592 Heart and neural crest derivatives expressed transcript 1 Hand1 2.56 NM_022498 Protein phosphatase 1, catalytic subunit, gamma isoform Ppp1cc 2.54 AJ011607 DNA primase, p58 subunit Prim2 2.53 BF412073 T-cell lymphoma invasion and metastasis 1 (predicted) Tiam1 2.53 NM_021693 SNF1-like kinase Snf1lk 2.53 AA944176 Leucyl-tRNA synthetase (predicted) Lars 2.52 NM_021869 Syntaxin 7 Stx7 2.50 AB022209 Heterogeneous nuclear ribonucleoprotein F Hnrpf 2.49 NM_031514 Janus kinase 2 Jak2 2.48 NM_019159 Synapsin II Syn2 2.48 BE109242 Kirsten rat sarcoma viral oncogene homologue 2 (active) Kras2 2.47 NM_031070 Nel-like 2 homolog (chicken) Nell2 2.46 NM_021576 5 nucleotidase Nt5 2.46 3 Downloaded from iovs.arvojournals.org on 09/30/2021 AW914809 Germ cell-less protein Gcl 2.46 AW531901 Guanine nucleotide binding protein, alpha q polypeptide Gnaq 2.45 NM_017038 Protein phosphatase 1A, magnesium dependent, alpha Ppm1a 2.45 isoform NM_021766 Progesterone receptor membrane component 1 Pgrmc1 2.45 J03819 Thyroid hormone receptor beta Thrb 2.43 AJ132846 Sodium-dependent neutral amino acid transporter Slc1a5 2.43 ASCT2 NM_031119 Sjogren syndrome antigen B Ssb 2.43 AI105205 CDW92 antigen Cdw92 2.43 AI409899 Solute carrier family 20, member 2 Slc20a2 2.42 NM_080904 ADP-ribosylation factor 3 Arf3 2.41 NM_012764 GATA binding protein 1 Gata1 2.41 NM_013013 Prosaposin Psap 2.41 0.49 AI575254 Sideroflexin 5 Sfxn5 2.40 NM_012744 Pyruvate carboxylase Pc 2.40 NM_021264 Ribosomal protein L35a Rpl35a 2.39 NM_057153 Oxr1 2.38 BF289092 Chloride intracellular channel 4 Clic4 2.38 NM_013090 Vesicle-associated membrane protein 1 Vamp1 2.38 BE108905 Nucleosome assembly protein 1-like 1 Nap1l1 2.37 NM_053989 dd5 2.36 NM_017325 Runt related transcription factor 1 Runx1 2.36 NM_053431 Quiescin Q6 Qscn6 2.33 NM_017153 Ribosomal protein S3a Rps3a 2.32 Y13972 MHC class I-like sequence Hlals 2.32 NM_031007 Adenylate cyclase 2 Adcy2 2.31 NM_013060 Inhibitor of DNA binding 2, dominant negative helix-loop- Id2 2.31 helix protein AF313411 Putative small membrane protein NID67 Nid67 2.31 NM_013151 Plasminogen activator, tissue Plat 2.30 NM_031777 Upstream transcription factor 1 Usf1 2.30 M69138 Nuclease sensitive element binding protein 1 Nsep1 2.30 NM_021694 Rho guanine nucleotide exchange factor (GEF) 1 Arhgef1 2.29 NM_031814 G protein-coupled receptor kinase interactor 1 Git1 2.29 NM_013043 Tgfb1i4 2.29 AF021923 Solute carrier family 24 (sodium/potassium/calcium Slc24a2 2.28 exchanger), member 2 NM_031788 RE1-silencing transcription factor Rest 2.27 NM_138505 Adrenergic receptor, alpha 2b Adra2b 2.27 M83676 RAB12, member RAS oncogene family Rab12 2.26 NM_017090 Guanylate cyclase 1, soluble, alpha 3 Gucy1a3 2.26 AW435429 G-protein signalling modulator 1 (AGS3-like, C. elegans) Gpsm1 2.26 NM_019280 Gap junction membrane channel protein alpha 5 Gja5 2.26 NM_053592 Deoxyuridine triphosphatase Dutp 2.25 4 Downloaded from iovs.arvojournals.org on 09/30/2021 BF525153 G1 to S phase transition 1 Gspt1 2.25 NM_017028 Mx2 2.25 NM_031977 Rous sarcoma oncogene Src 2.25 NM_053335 C-terminal binding protein 2 Ctbp2 2.25 NM_057114 Peroxiredoxin 1 Prdx1 2.24 NM_053471 Sema domain, transmembrane domain (TM), and Sema6b 2.24 cytoplasmic domain, (semaphorin) 6B NM_133419 Dyskeratosis congenita 1, dyskerin Dkc1 2.23 Y17326 Cleavage and polyadenylation specific factor 4 Cpsf4 2.23 NM_031684 Solute carrier family 29 (nucleoside transporters), Slc29a1 2.22 0.44 member 1 AI411375 V-ets erythroblastosis virus E26 oncogene homolog 2 Ets2 2.21 (avian) AI180349 Solute carrier organic anion transporter family, member Slco4a1 2.20 4a1 AF065161 Cytokine inducible SH2-containing protein Cish 2.20 BF284242 Adaptor-related protein complex 1, gamma 1 subunit Ap1g1 2.20 U17565 Mini chromosome maintenance deficient 6 (S.