Thermo Scientific Versette User’s Manual D09888 Revision 2.3 March 28, 2011 Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error- free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly. This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Release history: 21 December 2010, Rev. 1.9 Initial release. 6 January 2011, Rev. 2.0 17 January 2011, Rev. 2.1 - Added Cert. of Compliance. Updated SMC Calibration. 21 January 2011, Rev. 2.2 - Update to SMC Calibration. 28 March 2011, Rev. 2.3 - Update to Calibration sequences, and install of 2-stage system. For Research Use Only. Not for use in diagnostic procedures. PATENT PENDING © 2010-2011 Thermo Scientific Inc. All rights reserved. Declaration of Conformity Legal Identity: Thermo Fisher Scientific 81 Wyman Street Waltham, MA 02454 Authorized Representative: Thermo Fisher Scientific 22 Friars Drive Hudson, NH 03051 Equipment Identification Type of Equipment: Laboratory - Versette Automated Liquid Handler 650-01-BS, BASE TOWER Serial Numbers 1010 to 1500 We, Thermo Fisher Scientific, hereby declare that the devices mentioned above comply with the European Low Voltage Directive 2006/95/EC & 2004/108/EEC EMC Directive Common Technical Specifications EN55011 (2007) used for demonstration of EN61326-1 (2006) compliance: EN61000-3-2 (2000) + A2 (2005) EN61000-3-3 (1995) + A2 (2005) EN61010-1 (2001, 2nd ED.) Date of Validity: November 17, 2010 Name of Authorized Signatory: Michael Morrison Position Held in Company Operations Manager Location @ Signing Hudson, NH Signature: Thermo SCIENTIFIC WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product. Contact Thermo for further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country. WEEE Konformität Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen. Das Produkt ist durch folgendes Symbol gekennzeichnet: Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, bitte kontaktieren Sie Thermo für weitere Informationen. Thermo SCIENTIFIC Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont disponibles s'il vous plaît contacter Thermique pour les plus amples informations. Thermo SCIENTIFIC EMC Directive 89/336/EEC EMC compliance has been evaluated by TUV SUD America Inc. EN55011 Radiated & Conducted Emissions, Industrial, scientific, and medical (ISM) products and applications, Class B, Group 1 FCC Part 15 Class B Radiated and Conducted emissions, incluidng ICES-003, AS/NZA, and CISPR22, and EN55022. EN61000-3-2 & 3-3 Harmonics and Flicker EN61326-1 Including EN61000-4-2 - ESD EN61000-4-3 - Radiated Immunity EN61000-4-4 - EFT/Burst EN61000-4-5 - Surge EN61000-4-6 - Conducted Susceptibility EN61000-4-8 - Magnetics EN61000-4-11 - Dips/Dropouts IEC 60068-2-1 Cold Test IEC 60068-2-2 Dry Heat Test IEC 60068-2-3 Humidity Static EN61010-1 (sub) Safety Requirements Low Voltage Safety Compliance This device complies with Low Voltage Directive 73/23/EEC and harmonized standard EN 61010-1:2001. Thermo SCIENTIFIC FCC Compliance Statement THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED, INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION. CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device. Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one person alone to handle safely. Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument. Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument. For manufacturing location, see the label on the instrument. Contents Contents About this User Manual. .7 Intended users. 7 How to use this user’s manual. 7 Related Documentation . 8 Contacting Us . 8 Safety and Special Notices . 9 Safety symbols and markings . 10 Chapter 1 Introduction . 11 System Features. 12 Principle of Operation. 13 Advantages of using the Versette system . 15 Safety . 15 Chapter 2 System Overview. 23 General Description . 23 System Configuration . 24 Accessory Options . 25 Instrument layout . 29 Chapter 3 Installation . 39 Site requirements. 39 Requirements . 42 Precautions and limitations . 43 System installation procedure . 44 Use of a Reservoir with the Non-Contact Fill Option . 60 Using a Tip Wash Station . 62 Using a Pipette Tip Waste System. 64 Using Pipette Head Nests . 67 Using a Sealed Storage Tube Piercing Manifold . 69 Using a Barcode Reader. 72 Using a Vacuum Manifold . 76 Operational check . 77 Chapter 4 Operation . 79 Theory of operation. 80 Precautions and limitations . 80 Initial system startup . 81 Operation overview . 82 Programs . 83 Touchscreen operation . 84 Using Tube Adapters. 133 System Shutdown . 133 Thermo Scientific Versette User’s Manual Rev. 2.3 1 Contents ControlMate Software. 135 Chapter 5 Frequently Asked Questions . 139 Q&As . 139 Chapter 6 Calibration for SMC Pipetting Modules Only. 141 Calibrating the System Coordinates. 142 Required Equipment . 144 BEFORE CALIBRATION . 144 STEP 1: Install the SMC Pipetting Module and SMC Teach Tool . 148 STEP 2: Calibrate Stage XY . 155 STEP 3: Calibrate the Z-axis . 162 STEP 4: Calibrate the Serial Dilute coordinates . 168 STEP 5: Calibrate the Serial Dilute Nest Positions . 176 STEP 6: Calibrate Serial Dilute Syringe Axis (“S-Axis”) . 180 STEP 7: Eject Teach Tool and Verify Head and Tip Pickup. 184 Chapter 7 Calibration for NTC Pipetting Modules Only . 189 Calibrating the system coordinates . 190 Required Equipment . 192 BEFORE CALIBRATION . 192 STEP 1: Install the NTC Pipetting Module and NTC Teach Tool. 196 STEP 2: Calibrate Stage XY Coordinates. ..