Catalog # Aliquot Size U224-911-05 3 x 5 nmol U224-911-20 3 x 20 nmol U224-911-50 3 x 50 nmol UBE2I siRNA Set I siRNA duplexes targeted against three exon regions Catalog # U224-911 Lot # Z2109-25 Specificity Formulation UBE2I siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human UBE2I expression. shipped at room temperature. Product Description Reconstitution Protocol UBE2I siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human UBE2I mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_003345. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot C358B7.1; P18; UBC9 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or UBE2E3 (UBCH9) Protein U219-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2F Protein U220-30H µM can undergo up to 50 freeze-thaw cycles without UBE2G1 (UBC7) Protein U221-30H significant degradation. For long-term storage, it is UBE2H Protein U223-30H recommended that the siRNA is stored at -70oC. For most UBE2I Protein U224-30H favorable performance, avoid repeated handling and UBE2J1 Protein U225-30G multiple freeze/thaw cycles. UBE2K (UBC1) Protein U227-30H UBE2L6 (UBCH8) Protein U229-30H Scientific Background UBE2I or ubiquitin-conjugating enzyme E2I is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair. UBE21 UBE2I siRNA Set I also known as UBC9, plays a role DNA damage repair via siRNA duplexes targeted against three exon regions interaction with WT1, which is able to impose a block in cell cycle progression in eukaryotic cells (1). Listeria and Catalog # U224-911 probably other pathogens, dampens the host response Lot # Z2109-25 by decreasing the sumoylation level of proteins critical for Packaging Specifications 2.5 nmol/tube for 3 x 5nmol infection by targeting UBC21, an essential enzyme of the Format Lyophilized powder SUMO pathway. UBC21 and the SUMO pathway are Stability 1yr at -70oC from date of shipment crucial for proper nuclear architecture, accurate Storage & Shipping The lyophilized powder is stable for at chromosome segregation, and embryonic viability (2). least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Wang, Z.-Y. et.al: Molecular cloning of the cDNA and siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles chromosome localization of the gene for human without significant degradation. For ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: long-term storage, it is recommended 24811-24816, 1996. that the siRNA is stored at -70oC. For most favorable performance, avoid 2. Nacerddine, K. et.al The SUMO pathway is essential repeated handling and multiple for nuclear integrity and chromosome segregation in freeze/thaw cycles. mice. Dev. Cell 9: 769-779, 2005. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .