SUPPLEMENTARY DATA Supplementary Table 1. Mitochondrial and autophagy-focused post hoc analysis of islet cDNA expression microarrays from WT and Pdx1+/- mice. FC (Pdx1+/- NAME DESCRIPTION /Pdx1+/+) FDR Ndufs2 NADH dehydrogenase (ubiquinone) Fe-S protein 2 -1.769 5.48 Hspb7 heat shock protein family, member 7 (cardiovascular) -1.744 0 Got2 glutamate oxaloacetate transaminase 2, mitochondrial -1.695 26.38 Dhrs4 dehydrogenase/reductase (SDR family) member 4 -1.602 6.33 methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate Mthfd2 cyclohydrolase -1.597 20.72 Clec16a C-type lectin domain family 16, member A; KIAA0350 -1.583 6.33 Mrpl2 mitochondrial ribosomal protein L2 -1.487 40.08 Tomm20 translocase of outer mitochondrial membrane 20 homolog (yeast) -1.483 40.08 Hadh hydroxyacyl-Coenzyme A dehydrogenase -1.46 18.15 Pcx pyruvate carboxylase -1.448 7.18 Aldh1l2 aldehyde dehydrogenase 1 family, member L2 -1.434 5.48 Sars seryl-aminoacyl-tRNA synthetase -1.429 12.79 Rexo2 REX2, RNA exonuclease 2 homolog (S. cerevisiae) -1.427 40.08 Sars seryl-aminoacyl-tRNA synthetase -1.402 14.73 Bnip3l BCL2/adenovirus E1B interacting protein 3-like -1.398 31.02 Map1lc3b microtubule-associated protein 1 light chain 3 beta -1.394 31.02 Them2 thioesterase superfamily member 2 -1.389 8.77 Elac2 elaC homolog 2 (E. coli) -1.381 7.18 Pcx pyruvate carboxylase -1.377 8.77 Opa1 optic atrophy 1 homolog (human) -1.376 41.46 Acsl5 acyl-CoA synthetase long-chain family member 5 -1.375 41.46 Mrpl12 mitochondrial ribosomal protein L12 -1.363 31.02 Mrps24 mitochondrial ribosomal protein S24 -1.351 40.08 Aldh1b1 aldehyde dehydrogenase 1 family, member B1 -1.348 41.46 Vdac2 voltage-dependent anion channel 2 -1.334 41.46 Cox6c cytochrome c oxidase, subunit VIc -1.324 35.06 Brp44l brain protein 44-like -1.32 5.48 Hadh hydroxyacyl-Coenzyme A dehydrogenase -1.32 26.38 Cyb5r1 cytochrome b5 reductase 1 -1.314 41.46 2610524G07Rik RIKEN cDNA 2610524G07 gene -1.312 41.46 Gcat glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) -1.309 41.46 Sars seryl-aminoacyl-tRNA synthetase -1.307 12.79 Acsm2 acyl-CoA synthetase medium-chain family member 2 -1.301 26.38 Vdac1 voltage-dependent anion channel 1 -1.301 41.46 Mrpl39 mitochondrial ribosomal protein L39 -1.295 5.48 Yars2 tyrosyl-tRNA synthetase 2 (mitochondrial) -1.274 7.18 Comt catechol-O-methyltransferase -1.272 20.72 Phb2 prohibitin 2 -1.268 35.06 Nme3 expressed in non-metastatic cells 3 -1.265 11.31 methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate Mthfd1 cyclohydrolase, formyltetrahydrofolate synthase -1.263 18.15 Mtch1 mitochondrial carrier homolog 1 (C. elegans) -1.259 7.18 Mrpl2 mitochondrial ribosomal protein L2 -1.257 40.08 Tdh L-threonine dehydrogenase -1.256 41.46 Nme3 expressed in non-metastatic cells 3 -1.254 11.31 Cox6a1 cytochrome c oxidase, subunit VI a, polypeptide 1 -1.253 20.72 Crot carnitine O-octanoyltransferase -1.245 40.08 Gfm2 G elongation factor, mitochondrial 2 -1.244 41.46 ©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0376/-/DC1 SUPPLEMENTARY DATA Pdss1 prenyl (solanesyl) diphosphate synthase, subunit 1 -1.244 41.46 Nt5c3 5'-nucleotidase, cytosolic III -1.242 20.72 Akr1b7 aldo-keto reductase family 1, member B7 -1.238 31.02 2310056P07Rik RIKEN cDNA 2310056P07 gene -1.234 41.46 Nt5c3 5'-nucleotidase, cytosolic III -1.226 7.18 Pitrm1 pitrilysin metallepetidase 1 -1.226 5.48 Gpx4 glutathione peroxidase 4 -1.223 5.48 Gpd2 glycerol phosphate dehydrogenase 2, mitochondrial -1.221 20.72 Mrps25 mitochondrial ribosomal protein S25 -1.221 48.24 Me3 malic enzyme 3, NADP(+)-dependent, mitochondrial -1.215 43.77 0610009O20Rik RIKEN cDNA 0610009O20 gene -1.213 48.24 Maoa monoamine oxidase A -1.213 43.77 Fth1 ferritin heavy chain 1 -1.212 18.15 Nme3 expressed in non-metastatic cells 3 -1.21 12.79 4432406C05Rik RIKEN cDNA 4432406C05 gene -1.209 41.46 Acadl acyl-Coenzyme A dehydrogenase, long-chain -1.207 43.77 Mrps26 mitochondrial ribosomal protein S26 -1.207 26.38 Acadm acyl-Coenzyme A dehydrogenase, medium chain -1.203 48.24 Hadh hydroxyacyl-Coenzyme A dehydrogenase -1.203 43.77 Fh1 fumarate hydratase 1 -1.193 43.77 2610524G07Rik RIKEN cDNA 2610524G07 gene -1.191 31.02 Hsd17b10 hydroxysteroid (17-beta) dehydrogenase 10 -1.191 41.46 Rdh14 retinol dehydrogenase 14 (all-trans and 9-cis) -1.189 35.06 Sqrdl sulfide quinone reductase-like (yeast) -1.189 11.31 Park7 Parkinson disease (autosomal recessive, early onset) 7 -1.188 31.02 Idh2 isocitrate dehydrogenase 2 (NADP+), mitochondrial -1.187 43.77 0610006I08Rik RIKEN cDNA 0610006I08 gene -1.186 40.08 Bax Bcl2-associated X protein -1.183 41.46 Cox5a cytochrome c oxidase, subunit Va -1.179 41.46 Mrps18b mitochondrial ribosomal protein S18B -1.177 31.02 A830073O21Rik RIKEN cDNA A830073O21 gene -1.174 48.24 methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate Mthfd1 cyclohydrolase, formyltetrahydrofolate synthase -1.174 12.79 Ldha lactate dehydrogenase A -1.17 40.08 Ldha lactate dehydrogenase A -1.168 31.02 H2-Ke6 H2-K region expressed gene 6 -1.167 40.08 Mrpl12 mitochondrial ribosomal protein L12 -1.167 20.72 Ndufab1 NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1 -1.167 26.38 Chchd4 coiled-coil-helix-coiled-coil-helix domain containing 4 -1.166 41.46 Triap1 TP53 regulated inhibitor of apoptosis 1 -1.164 40.08 Usmg5 upregulated during skeletal muscle growth 5 -1.164 41.46 Acaa1b acetyl-Coenzyme A acyltransferase 1B -1.163 8.77 Oxnad1 oxidoreductase NAD-binding domain containing 1 -1.163 41.46 Mrps7 mitchondrial ribosomal protein S7 -1.162 43.77 Cox7a2l cytochrome c oxidase subunit VIIa polypeptide 2-like -1.158 11.31 Hbld2 HESB like domain containing 2 -1.158 41.46 Slc25a25 solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25 -1.157 41.46 Ndufb4 NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 -1.154 40.08 Mtch1 mitochondrial carrier homolog 1 (C. elegans) -1.152 41.46 Gpx1 glutathione peroxidase 1 -1.151 41.46 Mrpl30 mitochondrial ribosomal protein L30 -1.151 40.08 2310056P07Rik RIKEN cDNA 2310056P07 gene -1.149 48.24 Crls1 cardiolipin synthase 1 -1.149 35.06 Hagh hydroxyacyl glutathione hydrolase -1.149 41.46 ©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0376/-/DC1 SUPPLEMENTARY DATA Nme2 expressed in non-metastatic cells 2, protein -1.149 41.46 Hagh hydroxyacyl glutathione hydrolase -1.148 40.08 Mrpl46 mitochondrial ribosomal protein L46 -1.148 26.38 Rab1b RAB1B, member RAS oncogene family -1.144 40.08 Gbas glioblastoma amplified sequence -1.143 40.08 Tfam transcription factor A, mitochondrial -1.143 41.46 0710008K08Rik RIKEN cDNA 0710008K08 gene -1.142 41.46 2010107E04Rik RIKEN cDNA 2010107E04 gene -1.142 40.08 Auh AU RNA binding protein/enoyl-coenzyme A hydratase -1.142 43.77 Cry1 cryptochrome 1 (photolyase-like) -1.142 48.24 Ndufb9 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 -1.142 40.08 Ldha lactate dehydrogenase A -1.14 41.46 Tdh L-threonine dehydrogenase -1.14 43.77 1110001J03Rik RIKEN cDNA 1110001J03 gene -1.138 26.38 Cox7a2l cytochrome c oxidase subunit VIIa polypeptide 2-like -1.138 26.38 Rab1b RAB1B, member RAS oncogene family -1.138 40.08 Rhoa ras homolog gene family, member A -1.138 41.46 Zadh2 zinc binding alcohol dehydrogenase, domain containing 2 -1.136 35.06 Pnkd paroxysmal nonkinesiogenic dyskinesia -1.135 41.46 Brp44 brain protein 44 -1.133 48.24 Hint2 histidine triad nucleotide binding protein 2 -1.133 41.46 Mrrf mitochondrial ribosome recycling factor -1.133 35.06 Ndufa7 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (B14.5a) -1.133 41.46 Atpif1 ATPase inhibitory factor 1 -1.131 43.77 C1qbp complement component 1, q subcomponent binding protein -1.131 40.08 Guf1 GUF1 GTPase homolog (S. cerevisiae) -1.131 40.08 Idh3b isocitrate dehydrogenase 3 (NAD+) beta -1.131 26.38 Ndufa12l Ndufa12-like -1.131 41.46 Ndufs6 NADH dehydrogenase (ubiquinone) Fe-S protein 6 -1.13 20.72 Slc25a4 solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4 -1.129 43.77 Gpx1 glutathione peroxidase 1 -1.128 41.46 2010107E04Rik RIKEN cDNA 2010107E04 gene -1.125 41.46 Tspo translocator protein -1.124 43.77 Hmgcs2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 -1.122 58.28 Hagh hydroxyacyl glutathione hydrolase -1.121 41.46 1110007M04Rik RIKEN cDNA 1110007M04 gene -1.12 43.77 mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor Magmas signal transduction -1.12 40.08 Ndufa3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 -1.12 41.46 Rps14 ribosomal protein S14 -1.119 40.08 C1qbp complement component 1, q subcomponent binding protein -1.118 41.46 Auh AU RNA binding protein/enoyl-coenzyme A hydratase -1.117 35.06 Ict1 immature colon carcinoma transcript 1 -1.117 41.46 Nudt19 nudix (nucleoside diphosphate linked moiety X)-type motif 19 -1.117 43.77 Rab11b RAB11B, member RAS oncogene family -1.117 48.24 Cox7a2 cytochrome c oxidase, subunit VIIa 2 -1.115 40.08 Ndufb5 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 -1.115 40.08 Rab8b RAB8B, member RAS oncogene family -1.114 41.46 Yme1l1 YME1-like 1 (S. cerevisiae) -1.114 35.06 Nfxl1 nuclear transcription factor, X-box binding-like 1 -1.113 41.46 Rab8b RAB8B, member RAS oncogene family -1.113 41.46 Tomm20 translocase of outer mitochondrial membrane 20 homolog (yeast) -1.113 40.08 Mcart1 mitochondrial carrier triple repeat 1 -1.112 41.46 Mrpl15 mitochondrial ribosomal protein L15 -1.112 43.77 ©2015 American Diabetes Association.