ISAR INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) SAN FRANCISCO, CALIFORNIA | May 11th-15th, 2013 ISAR INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH Table of Contents Session Schedule . 3 Organization . 4 Contributors . .5 Program Highlights . 6 Agenda at a Glance . 7 Program Schedule . 12 Abstracts . 42 Author Index . 130 Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) 2 ISAR INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH Session Schedule Saturday, May 11, 2013 ■ Interactive Workshop: Drug Discovery and Development 101 ■ Opening Greetings and Welcome to San Francisco ■ Keynote Address ■ Opening Reception Sunday, May 12, 2013 ■ Oral Session 1: Mini-Symposium – Legacy of Tony Holý: Nucleotides in the Treatment and Prevention of Chronic Viral Infections ■ Gertrude Elion Award Lecture ■ Oral Session 2: Hepatitis Viruses and HIV ■ Poster Session 1: Retroviruses, Hepatitis Viruses, Respiratory Viruses, and Antiviral Methods Monday, May 13, 2013 ■ William Prusoff Young Investigator Award ■ Oral Session 3: Mini-Symposium – Strategies and Tactics in Drug Design ■ Oral Session 4: Influenza and Respiratory Infections ■ Poster Session 2: Herpesviruses, Poxviruses, Enteroviruses, Emerging Viruses, Other Antiviral Agents and Medicinal Chemistry Tuesday, May 14, 2013 ■ Oral Session 5: Herpesviruses and Poxviruses ■ Oral Session 6: Emerging Infections Wednesday, May 15, 2013 ■ Oral Session 7: Mini-Symposium – Prodrugs as a Tool in Drug Discovery and Development ■ Business Meeting ■ Oral Session 8: Clinical Symposium ■ Oral Session 9: Late Breaker and Shotgun Poster Presentations ■ ICAR Banquet and Reception Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) 3 ISAR INTERNATIONAL SOCIETY Organization FOR ANTIVIRAL RESEARCH International Society for Antiviral Research and Twenty-Sixth International Conference on Antiviral Research Officers President – Phillip Furman, St. Augustine, Florida, USA President-Elect – Robert W. Buckheit, Jr., Frederick, Maryland, USA Secretary – Graciela Andrei, Leuven, Belgium Treasurer – Dale L. Barnard, Logan, Utah, USA Past President – Joseph M. Colacino, South Plainfield, New Jersey, USA ISAR Conference Committee Conference Chair: Joseph M. Colacino, South Plainfield, New Jersey, USA Graciela Andrei, Leuven, Belgium Phillip Furman, St. Augustine, Florida, USA Masanori Baba, Kagoshima, Japan Johan Neyts, Leuven, Belgium Dale Barnard, Logan, Utah, USA Amy Patick, San Francisco, California, USA Robert Buckheit, Frederick, MD, USA Roger Ptak, Frederick, Maryland, USA Rhonda Cardin, Cincinnati, OH, USA Jack Secrist, Birmingham, AL, USA José A. Esté, Barcelona, Spain ISAR Program Committee Program Chair: Mark N. Prichard, Birmingham, Alabama, USA Graciela Andrei, Leuven, Belgium Chris Meier, Hamburg, Germany Robert Buckheit, Frederick, Maryland, USA Johan Neyts, Leuven, Belgium Karen Buckheit, Frederick, Maryland, USA Michael Sofia, New York, New York, USA Tomas Cihlar, Foster City, California, USA Paul M. Scola, Wallingford, Connecticut, USA Tracy Hartman, Frederick, Maryland, USA Donald Smee, Logan, Utah, USA Organizing Secretariats Courtesy Associates Graciela Andrei, ISAR Secretary 2025 M Street, NW, Suite 800, Washington, DC Professor, Rega Institute for Medical Research 20036, USA KU Leuven, Minderbroedersstraat 10 Phone: 1‐202‐973‐8690; Fax: 1‐202‐331‐0111 3000 Leuven, Belgium E‐mail: [email protected] Phone 32 16 33 73 72; Fax: 32 16 33 73 40 E‐mail: [email protected] The International Society For Antiviral Research (ISAR) The Society was organized in 1987 as a non‐profit scientific organization for the purpose of advancing and disseminating knowledge in all areas of antiviral research. To achieve this objective, the Society organizes an annual meeting. The Society is now in its twenty fifth year of existence, and has approximately 550 members representing 30 countries. For membership application forms or further information, please contact Dr. Graciela Andrei, Secretary, ISAR at the address noted above. Membership application forms will also be available at the Conference Registration desk, or from our website www.isar‐icar.com. Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) 4 ISAR INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH Contributors to the 26th International Conference on Antiviral Research Confirmed Sponsors as of April 16, 2013 Platinum Gilead Sciences, Inc., Foster City, CA, USA Gold GlaxoSmithKline, Inc., Research Triangle Park, NC, USA Silver AbbVie, Inc., North Chicago, Illinois, USA Biota Holdings Ltd., Notting Hill, Victoria, Australia Chimerix, Inc., Durham, NC, USA Flamma S.p.A., Bergamo, Italy Hetero Drugs Limited, Hyderabad, India Hoffmann-La Roche, Inc., Nutley, NJ, USA JCR Pharmaceutical Co., Ltd., Ashiya, Japan LabCorp – Monogram Biosciences, Inc., South San Francisco, CA, USA Novira Therapeutics, Inc., Doylestown, PA, USA Southern Research Institute, Birmingham, AL, USA Bronze Apath, LLC, Brooklyn, NY, USA Avexa Ltd., Richmond, Victoria, Australia Bristol-Myers Squibb, Wallingford, CT, USA Center for Drug Design, University of Minnesota, Minneapolis, MN, USA Elsevier B.V., Amsterdam, The Netherlands Express Biotech International, Thurmont, MD, USA Gemmus Pharma, Inc., San Francisco, CA, USA Idenix Pharmaceuticals, Inc., Cambridge, MA, USA ImQuest BioSciences, Inc., Frederick, MD, USA Medivir AB, Huddinge, Sweden PTC Therapeutics, Inc., South Plainfield, NJ, USA Toyama Chemical Co., Ltd., Tokyo, Japan ViroDefense, Inc., Rockville, MD, USA Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) 5 ISAR INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH Program Highlights Keynote Address “Know Thine Enemy: Using Virology and Immunology to Develop a Multifaceted Approach to Dengue Antivirals” Eva Harris, Ph.D. Saturday, May 11, 2013 4:15 pm – 5:30 pm Mini-Symposia Networking Events Legacy of Tony Hol´y: Opening Reception Nucleotides in the Treatment with light hors d’oeuvres and Prevention of Chronic Saturday, May 11, 2013 Viral Infections 5:30 pm– 7:30 pm Sunday, May 12, 2013 Location: Atrium 2-5 8:15 am – 12:00 pm New Member and First Time Attendee Strategies and Tactics Networking Event in Drug Design Sunday, May 12 Monday, May 13, 2013 6:30 pm – 7:30 pm 8:45 am – 11:45 am Location: Grand/Market Street Foyer Prodrugs as a Tool Career Happy Hour in Drug Discovery Monday, May 13 6:30 pm – 8:00 pm and Development Location: Sens Restaurant Wednesday, May 15, 2013 4 Embarcadero Center, Promenade Level 8:30 am – 11:30 am just outside of the Hyatt on the Lobby Level Clinical Symposium Wednesday, May 15, 2013 Women in Science Lunch* Tuesday, May 14 1:00 pm – 3:30 pm 12:30 pm – 2:30 pm Location: Seacliff D Conference Banquet Wednesday May 15, 2013 Reception 7:00 pm Dinner 7:30 pm – 9:30 pm Location: Grand Ballroom *space is limited and pre-registration is required. Please see the ICAR Registration Desk if you wish to attend or cancel your registration. Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) 6 ISAR INTERNATIONAL SOCIETY FOR ANTIVIRAL RESEARCH Agenda at a Glance Saturday • May 11, 2013 Interactive Workshop: Drug Discovery and Development 101 1:30 pm – 2:00 pm ■ Christos Petropolous, Ph.D. (LabCorp/Monogram Biosciences) – Will be discussing the requirements for resistance testing in drug development and the use of resistance data to define a compound’s mechanism of action. 2:00 pm – 2:30 pm ■ James M. McKim, Ph.D., DABT (CeeTox, Inc.) – “An In Vitro Model for Identifying Heart and Liver Specific Toxicity of Antiviral Drugs” 2:30 pm – 3:00 pm ■ Anthony Ham, Ph.D. (ImQuest BioSciences) –“The Importance and Role of Formulation in Antiviral Drug Development Programs” 3:00 pm – 3:30 pm ■ Martine Krauss, Ph.D. (Tobira Therapeutics) “Regulatory Milestones and Requirements in Antiviral Drug Development” 3:30 pm – 3:45 pm Panel Discussion Break 4:15 pm – 5:30 pm Keynote Address: “Know Thine Enemy: Using Virology and Immunology to Develop a Multifaceted Approach to Dengue Antivirals” ■ KEYNOTE SpeakeR: Eva Harris, Ph.D. – Professor of Infectious Diseases at UC Berkeley, and President of the Sustainable Sciences Institute. 5:30 pm – 7:30 pm Opening Reception Sunday • May 12, 2013 7:15 am – 8:15 am Continental Breakfast Oral Session I: Mini-Symposium: “Legacy of Tony Hol ´y : Nucleotides in the Treatment and Prevention of Chronic Viral Infections” 8:15 am – 8:30 am ■ Erik DeClercq, Ph.D., M.D. (Rega Institute) –Personal Note on the Contributions and Legacy of Tony Holý. 8:30 am – 9:00 am ■ John Martin, Ph.D. (Gilead) –Keynote Talk: A Tribute to Antonin Holý 9:00 am – 9:30 am ■ Robert Schooley, M.D. (UCSD) – “Tenofovir in the Treatment of HIV Infection.” 9:30 am – 10:00 am ■ Robert Grant, M.D. (UCSF) –”Tenofovir in the Prevention of HIV Infection.” Break 10:30 am – 11:00 am ■ Henry Chan, Ph.D. (The Chinese University of Hong Kong) — “Nucleotide Analogue in Chronic Hepatitis B – From Hope to Reality” 11:00 am – 11:30 am ■ Richard Whitley, M.D. (UAB) –”Nucleotides in the Treatment and Prophylaxis of Herpes and Other DNA VirusInfection.” 11:30am – 12:00 pm ■ Tomas Cihlar, Ph.D. (Gilead) – “Future Potential and Therapeutic Opportunities for Nucleoside Phosphonates.” Program and Abstracts of the 26th International Conference on Antiviral Research (ICAR) 7 Agenda at a Glance Lunch on Your Own 1:30 pm – 2:15 pm Gertrude