CONTENTS PART I : PLANT SCIENCES Phenotypic Screening and Single Marker Analysis for Brown Planthopper Resistance in 1 Rice (Oryza sativa L.) VG. ISHWARYA LAKSHMI, M. SREEDHAR, V. JHANSI LAKSHMI, C. GIREESH SANTOSHA RATHOD and S. VANISRI Correlation Studies Between Yield and Yield Attributes of Elite Rice Genotypes Transplanted 10 under Different Growing Environments SADRAS BHAVANA, T. RAMESH, P. RAGHUVEER RAO, B. BALAJI NAIK and CH. DAMODAR RAJU Aspergillus Flavus Soil Populations in Major Groundnut Growing Districts of Telangana State 17 SOMASHEKHAR KONDA, B. RAJESWARI, HARI KISHAN SUDINI, BHARATI BHAT, T. UMA MAHESWARI and D. SRINIVASA CHARY Genetic Variability, Heritability and Genetic Advance for Yield and Its Component Traits in 25 Germplasm Lines of Rice (Oryza Sativa L.) D. SHIVANI, FARZANA JABEEN, K. SUPRIYA, R. M. SUNDARAM, J. ARAVIND KUMAR, and R. ABDUL FIYAZ PART II : SOCIAL SCIENCES Kodangal Farmer Service Producer Company- A Constraint Analysis 30 D. A. RAJINI DEVI, R. VIJAYA KUMARI, T. LAVANYA, D. SRINIVASA CHARY and G. SAMUEL Impact of Precipitation on Yields of Major Crops in Telangana - A District Wise Analysis 38 CH. RAMYA SRI, K. SUHASINI, R. VIJAYA KUMARI, A. JANAIAH, SEEMA and D. SRINIVASA CHARY An Economic Analysis of Cultivation of Popular PJTSAU Rice Variety, RNR 15048 43 (Telangana Sona) Vis-À-Vis other Rice Varieties TAMILAZHAKI L, R. VIJAYA KUMARI, K. SUHASINI, A. JANAIAH, SEEMA, D. SRINIVASA CHARY and CH. DAMODAR RAJU PART III : VETERINARY SCIENCE Effect of Dietary Supplementation of Probiotics on Internal and External Egg Quality of White 49 Leghorn Layers G. RAVI SUDHAKAR, KRISHNA DAIDA, V. CHINNI PREETAM, B. PRAKASH and SRINIVAS GURRAM Effect of Supplementation of Detoxified Karanj Cake on Carcass Characteristics, Immune 53 Response and Biochemical Profile of Broiler Chicken D. KRISHNA, V. R. REDDY, CHINNI PREETAM V, M. V. L. N RAJU and SRINIVAS GURRAM PART IV : RESEARCH NOTES Economics of Gerbera under Polyhouse Cultivation in Rangareddy District of Telangana State 60 V.V.D. MANISHA, T. LAVANYA, R. VIJAYA KUMARI and K. SUPRIYA The Utilization Pattern of Rythu Bandhu Scheme (RBS): A Case Study in Nalgonda 65 District of Telangana. V. SNEHA, ALDAS JANAIAH, MD. ALIBABA and D. SRINIVASA CHARY Constraints/Challenges Faced by Farmers in Adoption of Micro Irrigation in Ranga Reddy 69 District of Telangana VISHWARADHYA, D. SRINIVASA REDDY, P. RADHIKA and K. SUPRIYA Standardisation and Quality Evaluation of Millet Based Nutribar 75 CR. RAJEESHA, CL. SHARON, SEEJA THOMACHAN PANJIKKARAN, ER. ANEENA, PS. LAKSHMY, K. RAMMYA MOLU and EM. NIVYA Research Article The J. Res. PJTSAU 49 (1&2) 1-9, 2021 PHENOTYPIC SCREENING AND SINGLE MARKER ANALYSIS FOR BROWN PLANTHOPPER RESISTANCE IN RICE (Oryza sativa L.) VG ISHWARYA LAKSHMI1, M. SREEDHAR2, V. JHANSI LAKSHMI3, C. GIREESH3, SANTOSHA RATHOD3 and S. VANISRI4* Department of Genetics and Plant Breeding, College of Agriculture Regional Sugarcane and Rice Research Station (RS & RRS), Rudrur, Nizamabad ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad Department of Molecular Biology and Biotechnology, Institute of Biotechnology Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500 030 Date of Receipt : 28-04-2021 Date of Acceptance : 06-05-2021 ABSTRACT Brown planthopper (BPH), Nilaparvata lugens (Stål) is a destructive pest that poses a threat to the food security of rice producing countries. In order to breed rice varieties with resistance to the brown planthopper, it is necessary to identify highly resistant germplasm sources. Seventy-three genotypes were evaluated using standard seed box screening technique over a period of two seasons that have shown varied levels of response reactions to the planthopper. Simultaneously, fifty-three microsatellite markers linked to BPH resistance were employed to find their association with BPH resistance. This association was estimated using regression based Single Marker Analysis (SMA) in Microsoft Excel. The significant marker trait associations (MTAs) were indicated by a P-value (< 0.05) along with corresponding R2 for each marker explaining the total phenotypic variation for BPH resistance accounted by markers. Statistically significant marker-trait associations were observed for five of the 39 polymorphic markers viz, RM335, RM510, RM589, RM8213 and RM494 indicating the possibility of significant putative genetic loci associated with BPH resistance on chromosomes 4, 6 and 10. The percentage of the total phenotypic variation as explained by the significant markers ranged from 1.0% to 24.4% suggesting the reliability of these genetic markers for further improvement of breeding for BPH resistance. Since these markers are linked with Bph3, Bph17(t), QBph6 and QBph10 genes/QTLs respectively, the presence of these genes in the resistant germplasm lines found in the study could be exploited through marker-assisted breeding programs for the development of BPH resistant cultivars. Brown planthopper, BPH, Nilaparvata resistant rice cultivars using molecular markers (Khush, lugens (Stål), (Hemiptera: Delphacidae) is considered 2001). Molecular markers, unlike morphological traits, to be the most devastating biological constraint that can show genotypic differences at the DNA level, impedes rice production across many countries in Asia making them more straightforward, accurate, and (Park et al., 2008). It causes direct damage to the efficient for germplasm characterization and selection. plants by sucking the phloem sap resulting in the Of all the available molecular markers, microsatellites drying of plants inciting hopperburn symptoms. The (Wu and Tanskley, 1993; Yang et al., 1994) are most most common approach for controlling the pest is widely used due to their co-dominant nature, high allelic through the application of insecticides. The chemical diversity, automated and easily assayed by means of control is not only costly but is also polymerase chain reaction. These microsatellite markers environmentally hazardous and leads to the have been efficiently utilized for genome mapping, development of insecticide resistance in BPH (Cheng marker aided breeding and for deriving marker-trait and Zhu, 2006; Jhansi Lakshmi et al., 2010). In order associations (McCouch et al., 2002; Yu et al., 2003; to establish a sustainable pest management strategy, Garris et al., 2005). the right combination of breeding and management Establishing marker-trait associations (MTAs) strategies must be found to reduce BPH’s ecological using phenotypic and marker data is highly useful in fitness while keeping the pest below economic investigating the genetic nature of a trait that can aid in threshold levels (Bosque-Perez and Buddenhagen, identification of the number and nature of genes/QTLs. 1992). Several markers reportedly linked to resistance against Host plant resistance has been considered BPH biotype 4 (Jairin et al., 2007; Liu et al., 2015; as one of the most effective ways of controlling the Sun et al., 2005; Ren et al., 2016; Naik et al., 2018) brown planthopper which can be deployed into have been utilized for establishing the association of email:[email protected] 1 ISHWARYA LAKSHMI et al. markers with resistance using Single Marker Analysis the rice fields of ICAR-IIRR. Pure culture was (Meshram et al., 2018). This analysis is a widely maintained in the glasshouse at a temperature of 25+ applied method that primarily detects the association 5°C with a relative humidity of 60+5% on 60 day old between marker genotype and trait value and also potted plants of susceptible variety TN1. Mass rearing gives an idea about its closeness to the QTL. Thus, in was done in cages. Adult females were released on the present study, marker analysis was done with the pre-cleaned potted plants placed in oviposition cages reported BPH resistance linked markers to identify their and after four days of egg laying, the gravid females linkage to the trait using single marker analysis. were removed and released on fresh TN1 plants for MATERIAL AND METHODS further egg laying in separate cages. Fresh plants were Plant Material : Seventy-three genotypes of rice provided for the hatched nymphs. The hatched nymphs including BPH gene differentials, resistant MAGIC lines, were utilized for experiments as and when they popular varieties, landraces and wild species were attained the desired age. A continuous pure culture of used in the study (Table 1). The gene differentials and BPH was maintained during the period of study. wild species of rice were obtained from ICAR- Indian Institute of Rice Research, Rajendranagar, Mass screening: The seeds of the germplasm lines Hyderabad, while, the MAGIC lines, varieties and were pre-soaked and sown in rows in 60×45×10 cm landraces were procured from the Institute of seed boxes along with resistant (PTB33) and Biotechnology, PJTSAU, Hyderabad. The lines TN1 susceptible (TN1) checks with 20 seedlings per row. and PTB33 were used as susceptible and resistant Twelve days old seedlings were infested with first instar checks respectively. nymphs of BPH at the rate of 6-8 per seedling. When Screening for BPH Resistance : The material was 90% of the plants in the susceptible check TN1 died, evaluated following the Standard Seed box Screening the germplasm lines were scored according to the Technique (SSST) for resistance reaction to BPH at Standard Evaluation System developed by the ICAR-Indian Institute of Rice Research (IIRR), International Rice Research Institute (IRRI, 2013)