Authors: Pallavi Subhraveti Ron Caspi Peter Midford Peter D Karp An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Ingrid Keseler Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. Gcf_000963905Cyc: Acetobacter aceti NBRC 14818 Cellular Overview Connections between pathways are omitted for legibility. Anamika Kothari a dicarboxylate a metal cation an amino phosphate a sulfonate phosphate acetate glycine betaine an amino an amino acid nitrate ammonium ammonium ammonium ethanolamine acid acid phosphate hydrogencarbonate gly predicted ABC RS00290 RS15790 RS13265 RS09910 Eat ActP RS05195 RS05160 RS12600 RS05170 transporter PstB RS04770 of phosphate a sulfonate glycine betaine phosphate nitrate ammonium ammonium ammonium ethanolamine acetate an amino an amino an amino phosphate a metal gly hydrogencarbonate acid acid acid cation phosphate a dicarboxylate Macromolecule Modification Aromatic Compound Biosynthesis Fermentation Amino Acid Degradation gln glutaminyl-tRNA Aminoacyl-tRNA Charging 6 ATP biosynthesis tRNA-uridine 2-thiolation N -(3-methylbut- a peptidoglycan queuosine at an [amino-group biosynthesis via transamidation a sulfurated a purine NADPH NAD + menadione NADH H + a nucleoside an L-asparaginyl- NAD(P) chorismate biosynthesis I pyruvate fermentation L-histidine 2-en-1-yl)- adenosylcobinamide RNA dimer (meso- glu position 34 carrier protein]- H + H + 3-(4-hydroxyphenyl) and selenation (bacteria) L-proline degradation I L-citrulline degradation L-glutamine triphosphate [tRNA Asn ] transhydrogenase to (S)-acetoin degradation II [sulfur carrier] 37 ribonucleoside Asp ADP degradation I adenosine diaminopimelate of a tRNA C-terminal-γ- glu D-erythrose pyruvate biosynthesis 5'-triphosphate F0F1 ATP in tRNA containing) (L-glutamate 5- 4-phosphate cys pyruvate NAD(P)(+) NADH-quinone N-acetyl- 3-deoxy-7- synthase: his pro L-citrulline gln DNA-directed aminoacyl- tRNA semialdehyde-2- tyr biosynthetic-type bifunctional transhydrogenase + F0F1 ATP + bifunctional ornithine tRNA (N6-isopentenyl oxidoreductase: RNA polymerase penicillin- yl)-L-glutamategamma- H H phosphoheptulonate bifunctional atpC atpD cysteine acetolactate Asp-tRNA(Asn) adenosylcobinamide (Re/Si-specific): tRNA glutamyl- synthase proline carbamoyltransferase: adenosine(37)-C2)- B7445_RS04235 subunit omega: binding glutamyl- synthase class II: aspartate B7445_RS10660 desulfurase: synthase: ilvB ilvN /Glu-tRNA(Gln) kinase/ B7445_RS00605 hydrolase: Q(34) gln urocanate dehydrogenase/L- argF methylthiotransferase B7445_RS04240 B7445_RS03140 protein phosphate an L-glutamyl- 3-deoxy- B7445_RS14720 transaminase/ B7445_RS10665 B7445_RS02005 (S)-2-acetolactate carbamoyl amidotransferase: adenosylcobinamide- B7445_RS00610 arfB synthetase an [amino-group glutamate gamma- MiaB: miaB B7445_RS04255 [+ 2 isozymes] 2: mrdA reductase: [tRNA Gln ] D-arabino- aspartate 4- B7445_RS10670 cysteine phosphate gatB gatA gatC phosphate B7445_RS00615 aminoacyl-tRNA GluQRS: carrier protein]- F0F1 ATP semialdehyde succinate nuoH nuoI argC H + H + Asp-tRNA(Asn) heptulosonate decarboxylase: F0F1 ATP desulfurase: [+ 5 isozymes] guanylyltransferase: hydrolase: gluQRS C-terminal-γ- synthase Asp-tRNA(Asn) dehydrogenase glutamine- dehydrogenase, Si-specific NAD(P)(+) B7445_RS04270 /Glu-tRNA(Gln) 7-phosphate 3-(4- B7445_RS13675 synthase: B7445_RS04505 glu cobU a peptidoglycan B7445_RS07925 (5-phospho- /Glu-tRNA(Gln) PutA: putA hydrolyzing hydrophobic transhydrogenase: nuoK nuoN RNA amidotransferase: 3-dehydroquinate hydroxyphenyl) B7445_RS11515 an [L-cysteine 4-imidazolone- (S)-1-pyrroline- with D,D cross- D-ala glutamyl- L-glutamyl)- amidotransferase: diacetyl carbamoyl- membrane anchor adenosyl- sthA NADH-quinone gatB gatA gatC synthase: pyruvate B7445_RS11520 desulfurase]-S- 5-propanoate 5-carboxylate links (meso- queuosine at L-glutamate H + RS04655 H + gatB gatA gatC phosphate protein: sdhD cobinamide oxidoreductase: [+ 5 isozymes] sulfanyl-L-cysteine diaminopimelate Asn a 5-phosphooxy- B7445_RS05855 B7445_RS11525 synthase: phosphate a purine asn tRNA position 34 tRNA charging acetoin reductase: nuoK nuoJ nuoI Asp B7445_RS11530 6 + containing) of a tRNA ammonium L-glutamyl- 3-dehydroquinate B7445_RS14175 carB carA N -(3-methylbut- ribonucleoside NADH NADP nuoH nuoG Gln 5'-deoxyadenosine an unsulfurated [tRNA ] 2-en-1-yl)-2- met 5'-diphosphate nuoE nuoC predicted ATP N-formimino- L-glutamate-5- carbamate [sulfur carrier] glu a tRNA glu type II 3- a [TusA sulfur- (S)-acetoin (methylsulfanyl) ABC Asp-tRNA(Asn) L-glutamate semialdehyde 37 dehydroquinate carrier protein]-S- adenosine urea transporter urea /Glu-tRNA(Gln) dehydratase: aroQ sulfanyl-L-cysteine in tRNA + ser a tRNA ser a tRNA Ala ala tRNA asp asp gly a tRNA Gly bifunctional NAD menadiol of urea amidotransferase: elongator glutamate-- glycine cleavage val a tRNA Val arg a tRNA Arg leu a tRNA Leu pro a tRNA Pro tyr a tRNA Tyr ile a tRNA Ile met initiator tRNA Met met thr a tRNA Thr his a tRNA His trp a tRNA Trp lys a tRNA Lys cys a tRNA Cys phe a tRNA Phe proline gatB gatA gatC tRNA Met 3-dehydroshikimate tRNA ligase: dehydrogenase/L- CO a [glycine-cleavage predicted Asn Gln N-formyl- 2 serine- alanine- B7445_RS06660 aspartate- glycine-- asn tRNA gln a tRNA a [TusD sulfur- glutamate gamma- 6 UDP-N-acetyl- undecaprenyl- a carboxy- a [bis(guanylyl a peptidoglycan shikimate complex H protein] N glycine cleavage system 3-aci- an L-cysteinyl- an L-cysteinyl- ABC an L-glutaminyl- valine-- -tRNA arginine-- leucine-- proline-- tyrosine-- -tRNA isoleucine-- methionine-- methionine-- glutamate-- threonine- -tRNA histidine-- tryptophan- lysine-- cysteine-- phenylalanine-- tRNA ligase: carrier protein]-S- L-glutamate semialdehyde α-D-glucosamine diphospho-N- adenylated- molybdopterin) a tRNA precursor dimer (E. faeciums) N-acetyl- urea urea dehydrogenase: -[(R)-lipoyl]-L-lysine aminomethyltransferase nitropropanoate cys cys Cys [tRNA Pro ] transporter [tRNA Gln ] sulfanyl-L-cysteine dehydrogenase [tRNA ] L-citrulline tRNA ligase: ligase: tRNA ligase: tRNA ligase: tRNA ligase: tRNA ligase: ligase: tRNA ligase: tRNA ligase: tRNA ligase: tRNA ligase: -tRNA ligase: tRNA ligase: -tRNA tRNA ligase: tRNA ligase: tRNA ligase: pheS B7445_RS01155 B7445_RS08225 N-formylglutamate GcvT: gcvT acetylmuramoyl- [ThiS sulfur- cofactor of urea B7445_RS01185 serS B7445_RS02180 B7445_RS02210 B7445_RS04345 B7445_RS04515 alaS B7445_RS06415 B7445_RS06505 B7445_RS06505 B7445_RS08795 ligase: thrS aspS B7445_RS08085 ligase: trpS B7445_RS09450 B7445_RS10025 B7445_RS10860 B7445_RS01160 [+ 2 isozymes] amidohydrolase: PutA: putA glycine cleavage system L-alanyl-γ-D- carrier protein] chaperone]- aminomethyl- nitronate penicillin- B7445_RS07005 aminomethyltransferase glutamyl-L-lysyl- L-cysteine ribonuclease D: binding acetylornithine Cys- predicted an L-methionyl- shikimate glu transferring monooxygenase: aminoacyl- an L-valyl- an L-seryl- an L-arginyl- an L-leucyl- an L-prolyl- an L-tyrosyl- an L-alanyl- an L-isoleucyl- an L-methionyl- an L-glutamyl- an L-threonyl- an L-aspartyl- an L-histidyl- an L-tryptophanyl- an L-lysyl- an L-cysteinyl- an L-phenylalanyl- an L-asparaginyl- an L-glutaminyl- a [TusE sulfur N-formylglutamate GcvT: gcvT D-alanyl-D-alanine B7445_RS15020 protein deacetylase: tRNA(Pro) ABC Gly UDP-N- B7445_RS08070 cysteine tRNA Val ser Arg Leu Pro Tyr Ala Ile [elongator Met Glu Thr asp His Trp Lys Cys Phe a glycyl-[tRNA ] Asn Gln glycine choline choline [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [initiator tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] [tRNA ] 34 carrier protein]-S amidohydrolase: [+ 2 isozymes] acetylglucosamine- desulfurase: cysteine ribonuclease 2: mrdA hydrolase: argE deacylase: transporter tRNA Met ] a uridine in tRNA dehydrogenase: nitronate -sulfanylcysteine B7445_RS08705 ybaK of choline gcvP -N-acetylmuramyl- monooxygenase: B7445_RS04505 desulfurase: D: rnd arfB acetylornithine a [glycine-cleavage a [glycine-cleavage (pentapeptide) B7445_RS01335 cysteine B7445_RS04505 a peptidoglycan aminoacyl-tRNA deacetylase: shikimate 3- tRNA 2- glu complex H protein] N 6 - pyrophosphoryl- argE glycine glycine complex H protein] N 6 desulfurase: cysteine tetramer with a D-ala hydrolase: RS00970 thiouridine(34) a nucleoside 5'- Pro betaine betaine phosphate L-methionine degradation aminomethyldihydrolipoyl- undecaprenol N- B7445_RS02005 desulfurase: a tRNA D,D cross-link B7445_RS07925 cys a tRNA synthase -dihydrolipoyl-L-lysine [unspecified monophosphate 3-phosphoshikimate 1- I (to L-homocysteine) L-lysine acetylglucosamine B7445_RS02005 (Enterococcus Carbohydrate Biosynthesis MnmA: mnmA 3-oxopropanoate nitrite degradation carboxyvinyltransferase: transferase: a thiocarboxylated- faecium) acetate L-citrulline met B7445_RS14645 products] predicted B7445_RS07385 ditrans,octacis- ala [ThiS sulfur- a [bis(guanylyl cys a tRNA Cys 5-enolpyruvoyl- a 2-thiouridine 34 UDP AMP O-antigen building blocks biosynthesis (E. coli) β methionine ABC peptidoglycan biosynthesis I (meso-diaminopimelate containing) Cell Structure Biosynthesis ADP-L-glycero- -D-manno CMP-3-deoxy-D- GDP-mannose biosynthesis α in tRNA undecaprenyldiphospho- carrier protein] ala molybdopterin)