HPLC Application ID No.: 20020 Quantification of Organophosphorus Pesticides by QuEchERS and LC/MS/MS Column: Synergi™ 4 µm Fusion-RP 80 Å, LC Column 50 x 2 mm, Ea Dimensions: 50 x 2 mm ID Order No: 00B-4424-B0 Elution Type: Gradient Eluent A: Add 5mL of 1M ammonium formate to 1L Water Eluent B: Add 5mL of 1M ammonium formate to 1L Methanol Gradient Step No. Time (min) Pct A Pct B Profile: 1 -5 95 5 2 0 95 5 3 2 95 5 4 6 50 50 5 12 10 90 6 14 10 90 7 16 95 5 8 17 95 5 Flow Rate: 250 mL/min Col. Temp.: 25 °C Detection: Tandem Mass Spec (MS-MS) @ (ambient) Detector Info: <a target="_blank" href="https://sciex.com/products/mass-spectrometers?utm_campaign=2019%20application%20search&utm_source=phenomenex&utm_medium=referral">SCIEX</a> Analyst Note: SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Fusion-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7556 - Holder Part No.: KJ0-4282 ©2021 Phenomenex Inc. All rights reserved. For more information contact your Phenomenex Representative at [email protected] Phenomenex products are available worldwide. www.phenomenex.com [email protected] Page 1 of 2 HPLC Application ID No.: 20020 Quantification of Organophosphorus Pesticides by QuEchERS and LC/MS/MS ANALYTES: 1 Acephate (MRM: 184/142.8) 41 Diazinon (MRM: 305/169) 2 Acephate (MRM: 184/94.9) 42 Diazinon (MRM: 305/153) 3 Demeton-S-methyl sulfone (MRM: 263/169) 43 Fenthion (MRM: 278.9/169) 4 Demeton-S-methyl sulfone (MRM: 263/109) 44 Fenthion (MRM: 278.9/246.9) 5 Parathion-methyl Methyl parathion (MRM: 263.9/232.1)45 Coumaphos (MRM: 363/226.9) 6 Parathion-methyl Methyl parathion (MRM: 263.9/125)46 Coumaphos (MRM: 363/307) 7 Dicrotophos (MRM: 238/112) 47 Phorate (MRM: 261/75) 8 Dicrotophos (MRM: 238/193) 48 Phorate (MRM: 261/199) 9 Demeton-S-methyl (MRM: 231/89) 49 Pirimiphos-methyl (MRM: 306.1/164.1) 10 Demeton-S-methyl (MRM: 231/61) 50 Pirimiphos-methyl (MRM: 306.1/108) 11 Dimethoate (MRM: 230/198.9) 51 Pirimiphos-ethyl (MRM: 334/198.1) 12 Dimethoate (MRM: 230/124.9) 52 Pirimiphos-ethyl (MRM: 334/182.1) 13 Mevinphos (MRM: 225/127) 53 Sulprofos (MRM: 323/219) 14 Mevinphos (MRM: 225/193) 54 Sulprofos (MRM: 323/247) 15 Dichlorvos (DDVP) (MRM: 221/109) 55 Chlorpyrifos (MRM: 350/198) 16 Dichlorvos (DDVP) (MRM: 221/127) 56 Chlorpyrifos (MRM: 350/97) 17 Phorate sulfoxide (MRM: 277/199) 57 Tokuthion (MRM: 344.9/241) 18 Phorate sulfoxide (MRM: 277/97) 58 Tokuthion (MRM: 344.9/133) 19 Famphur (Famophos) (MRM: 326/217) 20 Famphur (Famophos) (MRM: 326/281) 21 Naled (isomer 1) (MRM: 381/127) 22 Naled (isomer 1) (MRM: 381/109) 23 Azinphos-methyl (Gusathion) (MRM: 318/132) 24 Azinphos-methyl (Gusathion) (MRM: 318/160) 25 Malathion (MRM: 331/127) 26 Malathion (MRM: 331/99) 27 Triazophos (MRM: 314/162) 28 Triazophos (MRM: 314/119) 29 Azinphos-ethyl (MRM: 346/132.1) 30 Azinphos-ethyl (MRM: 346/160.1) 31 Tetrachlorvinphos (Tetrachlorovinphos) (MRM:366.9/127) 32 Tetrachlorvinphos (Tetrachlorovinphos) (MRM:368.9/127) 33 EPTC (internal standard) (MRM: 190.1/86) 34 EPTC (internal standard) (MRM: 190.1/128) 35 Chlorfenvinphos (MRM: 359/155) 36 Chlorfenvinphos (MRM: 359/99) 37 Parathion Ethyl parathion (MRM: 292.1/236.2) 38 Parathion Ethyl parathion (MRM: 292.1/94.1) 39 Sulfotep (MRM: 323/115) 40 Sulfotep (MRM: 323/171.1) ©2021 Phenomenex Inc. All rights reserved. For more information contact your Phenomenex Representative at [email protected] Phenomenex products are available worldwide. www.phenomenex.com [email protected] Page 2 of 2.