United States R«—rchand ^t-^A.l NSy^V EnvlronmMttal Protection Development Agency Symposium on Bioremediation of Hazardous Wastes EPA's Biosystems Technology Development Program Abstracts Holiday Inn Mart Plaza Chicago, IL May 5-6. 1992 Disclaimer v0 00 The information in this document has been funded wholly or in part by the U.S. Environmental rn Protection Agency (EPA) and has been reviewed in accordance with the EPA's peer and ^ administrative review policies and approved for presentation and publication. Mention of trade 0 names or commercial products does not constitute endorsement or recommendation for use. SYMPOSIUM ON BIOREMEDIATION OF HAZARDOUS WASTES: U.S. EPA'S BIOSYSTEMS TECHNOLOGY DEVELOPMENT PROGRAM Holiday Inn Mart Plaza Chicago, IL May 5-6,1992 TABLE OF CONTENTS Page Agenda ............................................................ 5 ^" 00 v~ Site Characterization .................................................. 11 m 0 Site Characterization at Pipeline Spill at Park City, Kansas: Q Estimating Hydraulic and Geochemical Constraints on Bioremediation ............. 13 Field Demonstration of Innovative Bioremediation Strategies for PCP and Creosote .................................................... 18 Case Study on Site Characterization at a TCE Plume: St. Joseph, Michigan, NPL Site ................................................... 25 Approaches to the Characterization of Trichloroethylene Degradation by Pseudomonas cepacia G4 ............................................... 27 Bioremediation Field Initiative ........................................... 33 Progress in the Field Applications of Bioremediation ........................... 35 Evaluation of Full-Scale In Situ and Ex Situ Bioremediation ...................... 36 of Creosote Wastes ................................................... Optimizing Bioventing in Shallow Vadose Zones and Cold Climates: Eielson AFB Bioremediation of a JP-4 Spill ................................. 45 Optimizing Bioventing in Deep Vadose Zones and Moderate Qimates: Hill AFB Bioremediation of a JP-4 Spill .................................... 53 Use of Nitrate to Bioremediate a Pipeline Spill at Park City, Kansas: Projecting from a Treatability Study to Full-Scale Remediation ................... 58 Design and Treatability Study of/n Situ Bioremediation of Chlorinated Aliphatics by Methanotrophs at St. Joseph, Michigan, NPL Site ................... 62 -1- TABLE OF CONTENTS (Continued) Bioremediation Field Initiative (Continued) Natural Anaerobic Treatment of a TCE Plume; St Joseph, Michigan, NPL Site 68 Proposed PCB Biodegradation Study at the Sheboygan River Confined Treatment Facility ............................................. 73 Performance Evaluation ................................................ 75 Development of Comparative Genotoridty Risk Methods for Evaluating Alternative Hazardous Waste Control Technologies ............................ 730 00 Performance of Bioventing at Traverse City, Michigan ......................... 80- Bioventing of a Gasoline Spill at Traverse City, Michigan: Practical Engineering Considerations .................... Process Research ..................................................... 93 Evaluation of Enhanced In Situ Aerobic Biodegradation of cis- and trans-1-Trichloroethylene and ds- and trans-l,2-Dichloroethylene by Phenol-Utilizing Bacteria ......................... 95 Fundamental Studies on the Treatment of VOCs in a Biofilter ................... 101 Fungal Treatment of Pentachlorophenol .................................... 103 Anaerobic Degradation of Highly Chlorinated Dibenzo^-Diorins and Dibenzofurans ....................................................... 108 Bacterial Degradation of Trichloroethylene in a Gas-Phase Biorcactor .............. 113 Anaerobic Biodegradationiation of 5-Chlorovanillate as a Model Substrate for the Bioremediation of Paper-Milling Waste ............................... 117 Methanogenic Degradation of Hctcrocyclic Aromatic Compounds by Aquifer-Derived Microcosms ............................................ 120 Chemical Interactions and pH Profiles in Microbial Biofilms ..................... 126 Decontamination of PCB-Contaminated Sediments through the Use of Bioremediation Technologies ............................................ 129 2 TABLE OF CONTENTS (Continued) Process Research (Continued) Bioremediation of Soils and Sediments Contaminated with Aromatic Amines ......... 132 Development of Small-Scale Evaluation Techniques for Fungal Treatment of Soils ..... 135 Modeling ........................................................... 139 Prediction of GAC Adsorptive Capacity with and without _ Molecular Oxygen .................................................... 141 Development of Tools for Monitoring Biofilm Processes ........................ 145 T-" 0 Poster Session ....................................................... 147 0 List of Poster Presentations ............................................. 149 Clearance and Pulmonary Inflammatory Response after Intranasal Exposure of C3h/HeJ Mice to Biotechnology Agents ........................... 151 Development of Aerobic Biofilter Design Criteria for Treating VOCs .................................................... 152 Sequential Anaerobic-Aerobic Treatment of Contaminated Soils and Sediments ................................................... 154 Influence of Low Levels of Nonionic Surfactants on the Anaerobic Dechlorination of Hexachlorobenzene .............................. 157 Characterization of Microorganisms, Microbial Consortia, and Microbial Processes for the Reductive Dechlorination of Hazardous Wastes ....................................... 160 Effects of Metals on the Reductive Dechlorination of Chlorophenols ............... 163 Evaluation of Anaerobic Respirometry to Quantify Intrinsic Anaerobic Biodegradation Kinetics of Recalcitrant Organic Compounds ..................... 165 Anaerobic Transformation and Degradation of Chlorobenzoates and Chlorophenols under Four Reducing Conditions ........................... 166 Characterization of Biofilter Microbial Populations ............................ 168 U.S. Environmental Protection Agency SYMPOSIUM ON BIOREMEDIATION OF HAZARDOUS WASTES: U.S. EPA'S BIOSYSTEMS TECHNOLOGY DEVELOPMENT PROGRAM Holiday Inn Mart Plaza Chicago, IL May S-6, 1992 AGENDA DAY ONE . Tuesday. May 5.1992 0 7:30 AM SEMINAR REGISTRATION (^ 8:30 AM Introduction rr\ Ralph R. Bauer, Deputy Regional Administrator, U.S. ° Environmental Protection Agency (U.S. EPA), Chicago, IL 0 8:40 AM Overview of Biosystems Technology Development Program John E. Rogers, U.S. EPA, Athens, GA 9:00 AM Utilization of Innovative Technologies Walter Kovalick, Director, Technology Innovation Office, Office of Solid Waste and Emergency Response, U.S. EPA, Washington, DC Site Characterization Session Chairperson • John Wilson 9:15 AM Site Characterization at Pipeline Spill at Park City, Kansas: Estimating Hydraulic and Geochemical Constraints on Bioremediation Lonnie Kennedy, Coastal Remediation Company, Norman, OK 9:45 AM Field Demonstration of Innovative Bioremediation Strategies for PCP and Creosote Jim Mueller, SBP Technologies. Inc., Stone Mountain, GA 4X^16^ Q. M- ^04-) ^4--^c 10:15 AM BREAK ^ ^ 10:30 AM Case Study on Site Characterization at a TCE Plume; St. Joseph, Michigan, NPL Site John Kuhns,* U.S. EPA, Chicago, IL; Peter Kitanidis, Stanford University, Stanford, CA * Indicates presenter when there are several authors. -5- 10:50 AM Approaches to the Characterization ofTrichloroethylene Degradation by Paeudomonas eepacia G4 Charles Somerville and Michael Reagin, Technical Resources, Inc. (TRI), Gulf Breeze, FL; Malcom Shields,* University of West Florida Center for Environmental Diagnostics and Bioremediation, Gulf Breeze, FL; Robert Gergcr, U.S. EPA. Gulf Breeze, FL Bioremediation Field Initiative Session Chairperson - Fran Kremer 11:20 AM Progress in the Field Applications of Bioremediation Fran Kremer, U.S. EPA. Cincinnati, OH 11:35 AM Evaluation of Full-Scale In Situ and Ex Situ Bioremediation of Creosote Wastes John Matthews and Bert Bledsoe, U.S. EPA, Ada, OK; Ronald Sims,* Utah State University, Logan, UT ,''" 12:05 PM LUNCH <r- r^ 1:10 PM Optimizing Bioventing in Shallow Vadose Zones and Cold Climates: 0 Eielson AFB Bioremediation of a JP-4 Spill 0 Greg Sayles,* U.S. EPA, Cincinnati, OH; Robert Hinchee, Battelle, Columbus, OH; Richard Brenner, U.S. EPA, Cincinnati, OH; Catherine Vogel, U.S. Air Force, Tyndall Air Force Base, FL; Ross Miller, U.S. Air Force, Brooks Air Force Base, TX 1:30 PM Optimizing Bioventing in Deep Vadose Zones and Moderate Climates: Hill AFB Bioremediation of a JP-4 Spill Greg Sayles,* U.S. EPA, Cincinnati, OH; Robert Hinchee, Battelle, Columbus, OH; Richard Brenner, U.S. EPA, Cincinnati, OH; Catherine Vogel, U.S. Air Force. Tyndall Air Force Base, FL; Ross Miller, U.S. Air Force, Brooks Air Force Base, TX 1:50 PM Use of Nitrate to Bioremediate a Pipeline Spill at Park City, Kansas: Projecting from a Treatability Study to Full-Scale Remediation Steve Hutchins, U.S. EPA, Ada, OK 2:20 PM BREAK Bioremediation Field Initiative (continued^ Session Chairperson - John Wilson 2:35 PM Design and Treatability Study of In Situ Bioremediation of Chlorinated Aliphatics by Methanotrophs at St. Joseph, Michigan, NPL Site Lewis Semprini,* Perry McCarty, Mark Dolan, Margaret Lang, Thomas McDonald, Jaeho Bae, and Peter Kitanidis, Stanford University, Palo Alto, CA * Indicates presenter when there are several authors. -6- 3:05 PM