Report of the Tomato Genetics Cooperative Volume 57 September 2007 THIS PAGE IS INTENTIONALLY BLANK Report of the Tomato Genetics Cooperative Number 57- September 2007 University of Florida Gulf Coast Research and Education Center 14625 CR 672 Wimauma, FL 33598 USA Foreword The Tomato Genetics Cooperative, initiated in 1951, is a group of researchers who share and interest in tomato genetics, and who have organized informally for the purpose of exchanging information, germplasm, and genetic stocks. The Report of the Tomato Genetics Cooperative is published annually and contains reports of work in progress by members, announcements and updates on linkage maps and materials available. The research reports include work on diverse topics such as new traits or mutants isolated, new cultivars or germplasm developed, interspecific transfer of traits, studies of gene function or control or tissue culture. Relevant work on the Solanaceous species is encouraged as well. Paid memberships currently stand at approximately 121 from 21 countries. Requests for membership (per year) US$20 to addresses in the US and US$25 if shipped to addresses outside of the United States should be sent to Dr. J.W. Scott, [email protected]. Please send only checks or money orders. Make checks payable to the University of Florida. We are sorry but we are NOT able to accept cash or credit cards. Cover. Design by Christine Cooley and Jay Scott. Depicted are “Tomatoes of the Round Table” as opposed to “Knights of the Round Table”. This year we celebrate the 50th Anniversary of the Tomato Breeders Roundtable (TBRT). See this year’s feature article for information on the history of the Tomato Breeders Roundtable. THIS PAGE IS INTENTIONALLY BLANK TABLE OF CONTENTS TGC REPORT 57, 2007 ________________________________________________________________________________ Foreword ……………………………………………………………………………………………………..1 Announcements ………………………………………………………………………………………....…3 Feature Article ………………………………………………………………………………………………7 Research Reports Occurrence of Anthocyanin in Cultivated Tomato Boches, Peter S. and Myers, James R. ……………………………………………………….………14 Thifensulfuron susceptibility in tomatoes and possible linkage with blossom end rot Dick, Jim ……………………………………………………………………………………………….… 20 Co-dominant SCAR marker for detection of the begomovirus-resistance Ty-2 locus derived from Solanum habrochaites in tomato germplasm Garcia, Brenda E., Graham, Elaine, Jensen, Katie S., Hanson, Peter, Mejía, Luis and Maxwell, Douglas P. …………………………………………………………………….……..…. 21 Co-dominant SCAR Markers for Detection of the Ty-3 and Ty-3a Loci from Solanum chilense at 25 cM of Chromosome 6 of Tomato Ji, Yuanfu, Salus, Melinda S., van Betteray, Bram, Smeets, Josie, Jensen, Katie S., Martin, Christopher T., Mejía, Luis, Scott, Jay W., Havey, Michael J. and Maxwell, Douglas P. ………………………………………………………………………………..…. 25 Antioxidants in Faculty Tomatoes Kedar, N. ……………………………………………………………………………………………….. 29 Evaluation of PCR-based markers for scanning tomato chromosomes for introgressions from wild species Martin, Christopher T., Salus, Melinda S., Garcia, Brenda E., Jensen, Katie S., Montes, Luis, Zea, Carolina, Melgar, Sergio, El Mehrach, Khadija, Ortiz, Julieta, Sanchez, Amilcar, Havey, Michael J., Mejía, Luis and Maxwell, Douglas P. …………………………………………. 31 Identification of molecular markers linked to a new Tomato spotted wilt virus resistance source in tomato Price, David L., Memmott, Frederic D., Scott, Jay W., Olson, Steve M. and Stevens, Mikel R. 35 Evaluation of a co-dominant SCAR marker for detection of the Mi-1 locus for resistance to root-knot nematode in tomato germplasm Seah, Stuart, Williamson, Valerie M., Garcia, Brenda E., Mejía, L., Salus, Melinda S., Martin, Christopher T. and Maxwell, Douglas P. ………………………………………………….. 37 Transgenic Lycopersicon ssp. plants expressing the gene for human acidic fibroblast growth factor Stoykova, Petya, Radkova, Mariana, Stoeva-Popova, Pravda, Wang, Xingzhi and Atanassov, Atanas ………………………………………………………………………………….… 41 Varietal Pedigrees ‘Fla. 8153’ tomato hybrid; Fla. 8059 and Fla. 7907 breeding lines. Scott, J.W., Baldwin, E.A., Klee, H.A., Olson, S.M., Bartz, J.A., Sims, C.A. and Brecht, J.K.. .......……………………............................................................................. 44 Gc9, Gc171, and Gc173 begomovirus resistant inbreds. Scott, J.W. and Schuster, D.J..........……………………………………………....…………… 45 Stock Lists …………………………………………………………………………………….…….….… 47 Membership List …………………………………………………………………………….……….….. 74 Author Index ………………………………………………………………………………….………….. 79 THIS PAGE IS INTENTIONALLY BLANK ANNOUNCEMENTS TGC REPORT 57, 2007 ________________________________________________________________________________ From the editor: Happy Autumn to the TGC membership. In Florida this is a nice time of year because it begins to finally cool off from the 4-5 months of hot weather. Otherwise it is a nice time of year because you have the latest TGC report! This is my fifth report as editor for those who are keeping track. My thanks goes to Dolly Cummings who does a lot of the work in preparation of the TGC report and keeping our spreadsheets and mailings in order. Christine Cooley as well as Dolly have been helping with the website updates. My contact information has not changed at all for the first time in quite awhile: Jay W. Scott, Ph.D. Gulf Coast Research & Education Center 14625 CR 672 Wimauma, FL 33598 USA Phone; 813-633-4135 Fax; 813-634-0001 Email; [email protected] Do not hesitate to contact me if you have any questions or concerns. Also be sure to check our website for additional TGC information: http://tgc.ifas.ufl.edu/. We still have some work to do on the electronic volumes but all are available online and fairly complete searches can be done by keyword. We will continue to finalize this process in the coming year. Also, you see we are featuring the Tomato Breeders Roundtable in this issue and the website will now (or very soon) contain abstracts of the TBRT presentations. Thanks to all who have submitted reports this year and I hope you will consider submitting reports in the future. If there has been a change in your contact information please email me about it. Good luck in your 2007-2008 tomato pursuits. Jay W. Scott Managing Editor P.S. from Dolly: Please check your contact information in the ‘Membership List’ section to be sure that all the information we have is complete and correct. Thanks! 3 ANNOUNCEMENTS TGC REPORT 57, 2007 ________________________________________________________________________________ Upcoming Meetings 2nd International Symposium on Tomato Disease, October 8-12, 2007 (better hurry!). http://www.2istd.ege.edu.tr/index.html Tomato Disease Workshop, October 24-26, 2007, Williamsburg, VA. http://www.cpe.vt.edu/tdw. Tomato Breeders Roundtable, November 4-7, 2007, Penn State U. http://tomatoroundtable07.psu.edu/ 5th Solanceae Genome Workshop 2008, Oct 1-8, 2008, Cologne, Germany. Information will be posted at mpiz website or contact Christiane Gebhardt, [email protected], the local organizing committee representative. 4 ANNOUNCEMENTS TGC REPORT 57, 2007 ________________________________________________________________________________ Grant Opportunity: Request for Proposals for Tomato Germplasm Evaluation Funding is expected to be available again in fiscal year 2008 for evaluation of tomato germplasm. Proposals must be submitted through the Crop Germplasm Committee (CGC). All proposals will be evaluated according to the national need for evaluation data, the likelihood of success, and the likelihood that the data will be entered into GRIN and shared with the user community. Evaluation priorities established by the CGC (Table 1) will provide review criteria. When all other factors are equal, preference for funding will be given to supporting those proposals forwarded by CGCs that have not received prior funding. Proposals will be reviewed by the CGC and forwarded to the USDA for consideration. Proposals must be returned to the CGC Chair by November 9, 2007 so that reviews and rankings, can be forwarded to the USDA in Beltsville by December 3, 2007. Because of limited funds, the USDA cannot support all proposals submitted. Consequently, please be very frugal in your request for funds. In recent years, the USDA has caped budget allocations in the range of $15,000-$18,000 per project annually. The proposal format is outlined below. Please submit proposals electronically as a PDF file to David Francis, CGC Chair, [email protected]. I. Project title and name, title of evaluators. II. Significance of the proposal to U.S. agriculture. III. Outline of specific research to be conducted including the time frame involved – include the number of accessions to be evaluated. IV. Funding requested, broken down item by item. Budgets should follow USDA form ARS454 as funding will be in the form of a specific cooperative agreement. No overhead charges are permitted. V. Personnel: A. What type of personnel will perform the research (e.g. ARS, State, or industry scientist; postdoc; grad student, or other temporary help). B. Where will personnel work and under whose supervision. VI. Approximate resources contributed to the project by the cooperating institution (e.g. facilities, equipment, and funds for salaries). 5 ANNOUNCEMENTS TGC REPORT 57, 2007 ________________________________________________________________________________ Table 1. Crop Germplasm Committee Priorities for Tomato Type Priority Description Bacterial Diseases High Bacterial canker