Insect Hormones (19th Ecdysone) Interna5onal Workshop, 2013 July 21 to 26 University of Minnesota Minneapolis USA Workshop website: hKp://www.cbs.umn.edu/gcd/insect-hormones-ecdysone-workshop Thank you to our sponsors: U of M sponsors: John and Margaret Ordway Chair Department of Developmental Biology Genetics, Cell Biology and Development Local Organizers: Mary Muwahid Michael B. O’Connor MaryJane Shimell International Insect Hormone (19th Ecdysone) Workshop 2013 July 22-26, University of Minnesota Minneapolis, Minnesota, USA Monday, July 22, 2013 Johnson Great Room at McNamara Center, 200 Oak Street S.E., Minneapolis Campus 8:00 - 8:45 am Registration and Breakfast (outside Johnson Room in Memorial Hall) 8:50 - 9:00 am Welcome: Mike O’Connor, University of Minnesota Session I: Nuclear hormone receptors (Chair: Ron Hill) 9:00 - 9:25 Ron Hill, CSIRO, Australia. Mutagenesis of Recombinant Ecdysone Receptor Ligand Binding Regions 9:30 - 9:55 Masako Asahina, Biology Center, U. of South Bohemia, Czech Republic Sumoylated NHR-25/Ftz F1/NR5A regulates cell fate in C. elegans 10:-00 - 10:25 Guy Smagghe, Ghent University, Belgium Cell based reporter assay for identifying EcR agonist/antagonists 10:30 - 11:00 Coffee break Session II: Hormone Regulation of Metabolism (Chair: Jason Tennessen) 11:00 - 11:25 Jason Tennessen, University of Utah, USA Drosophila larval development uses aerobic glycolysis to support rapid growth 11:30 - 11:55 Arpan Gosh, University of Minnesota, USA Activin signaling regulates sugar homeostasis and pH balance in Drosophila 12:00 - 12:25 Hui Bai, Brown University, USA Juvenile hormone interacts with Insulin signaling to control lipid metabolism 12:30 - 2:00 Lunch Coffman Union 2:00 - 3:30 Poster Session I – Memorial Hall, McNamara Center (poster stands will be up until Tuesday evening) Session III Neuroendocrine signaling (Chair: Ryuske Niwa) 3:30-3:55 Ryuske Niwa, University of Tsukuba, Japan Regulation of ecdysteroid biosynthesis through serotonin-producing neurons 4:00 - 4:25 Qiuxiang Ou University of Alberta, Canada Drosophila neurotropin Spatzle 5 is required for ecdysone synthesis 4:30 - 4:55 Shi-Hong Gu Museum of Natural Science, Taiwan Role of Bombyx AMPK in PTTH-stimulated Ecdysteroidogenesis 5:00 - 5:25 Masatoshi Iga, University of Toyko, Japan PDF mediated regulation of ecdysone biosynthesis in the PG of Bombyx 5:30 - 5:55 Yuya Ohhara, University of Shizuoka, Japan Autocrine monoaminerigic signaling triggers ecdysone biosynthesis in Drosophila Dinner on the town Tuesday, July 23, 2013 8:00 – 8:30 am Breakfast: Comstock Dinning Hall or outside meeting room Session IV: Hormones and Trafficking events (Chair: Andrew Anders) 8:30 - 8:55 Andrew Andres, University of Las Vegas, USA Using E23 as genetic tool to block tissue-specific responses to 20-hydroxyecdysone in Drosophila. 9:00 - 9:25 Samantha Hindle, UCSF, USA ABC Transporters Regulate CNS Chemoprotection through Ecdysone signaling at the Blood Brain Barrier 9:30 - 9:55 Rosa Barrio, CIC bioGUNE, Spain Steroid synthesis and Growth control 10:00 - 10:30 Coffee Break Session V: Developmental timing (Chair: Jim Truman) 10:30 - 10:55 Jim Truman, HHMI Janelia Farms, USA The role of molt timers in determining instar length in Manduca Sexta 11:00 - 11:25 Hitoshi Ueda, Okayama University, Japan Control of developmental timing by fat body transcription factors in Drosophila 11:30 - 11:55 Yuichiro Suzuki, Wellesley College, USA Ventral veins lacking interacts with JH and Ecdysone to influence the timing of metamorphosis 12:00 - 12:25 Kim Rewitz, University of Copenhagen, Denmark Feedback circuits shape the metamorphosis-inducing steroid pulse 12:30 - 2:00 Lunch 2:00 - 3:00 Poster Session II Session VI: Hormonal control of body size and organ growth/remodeling (Chair: Sheng Li) 3:00 - 3:25 Sheng Li, Chinese Academy of Sciences, China Fat Body remodeling and it hormonal regulation in Drosophila Tuesday, July 23, 2013 continued 3:30 - 3:55 Naoki Okamoto, RIKEN center for Developmental biology, Japan A secreted decoy of InR antagonizes insulin/IGF signaling to restrict body growth 4:00 - 4:25 Rewatee Gokhale, Michigan State University, USA Ecdysone signaling Mediates intra-organ growth coordination in Drosophila 4:30 - 4:55 Wu-Min Deng, Florida State University, USA Tissue repair through cell competition and compensatory cellular hypertrophy in postmitotic epithelia 5:00 - 5:30 Takashi Koyama, Instituto Gulbenkian de Ciencia, Portugal Nutrition regulates body size through FoxO-Ultraspiracle mediated ecdysone synthesis Light Rail trip to Mall of America for Dinner and shopping? Wednesday, July 24 8:00 - 8:30 Breakfast: Comstock Dinning Hall or outside meeting room Session VII: Hormones and Behavior-Nongenomic 20-E responses (Chair: Naoki Yamanaka) 8:30 - 8:55 Naoki Yamanaka, University of Minnesota, US PTTH coordinates physiological and behavioral transitions during Drosophila development 9:00 - 9:25 Xiao-Fan Zhao, Shandong University, China Regulation of cyclin-dependent kinase 10 phosphoryation by steroid hormone 20-E through GPCR signaling 9:30 - 9:55 Hiroshi Ishimoto, University of Iowa, USA A novel action of the steroid hormone ecdysone on behavioral plasticity in adult Drosophila 10:00 - 10:30 Break 10:30 - 10:55 Hiroko Sano, Kurume University, Japan Analysis of the peripheral tissue-derived peptides, CCHamide-1 and 2 and dRYamide 1 and 2 in Drosophila 11:00 - 11:25 Taketoshi Kiya, Kanazawa University, Japan Identification of Hr38 as a conserved neural activity-induced gene in insect brains 11:30 - 12:15 Box Lunches Great Hall 12:15 Board bus for Harriet Island 12:30 - 1:30 Transportation to Harriet Island in St. Paul Lorenz Bus Service will be arriving at 12:15 and will depart McNamara at 12:30 1:30 - 2:00 pm Board the Betsey Northup Harriet Island, St. Paul 2:00 - 5:00 Sail on the Betsey Northup 5:00 Transportation back to McNamara Center Lorenz Bus Service Dinner on the town Thursday, July, 25 8:00 - 8:30 Breakfast: Comstock Dinning Hall or outside meeting room Session VIII JH signaling (Chair: Xavier Belles) 8:30 - 8:55 Xavier Belles, CSIC-UPF Barcelona, Spain A single miRNA drives hemimetabolan metamophosis to a right end 9:00 - 9:25 Tetsuro Shinoda, NIAS Tsukuba, Japan Hormonal regulation of Kruppel homolog 1 and its mechanisms or repressing metamorphosis in Bombyx 9:30 - 9:55 Takahiro Shiotsuki, NIAS Tsukuba, Japan The characteristics of JH epoxide hydrolase genes in insects 10:00- 10:30 Coffee break Session IX JH signaling continued and JH-Ecdysone-interactions (Chair, Lynn Riddiford) 10:30 - 10:55 Lynn Riddiford, HHMI Janelia Farm USA Roles of JH receptors Methoprene-tolerant and Germ cell-expressed in Drosophila larval development and metamorphosis 11:00 - 11:25 David Martin, CSIC-UPF Barcelona, Spain Ecdysone meets juvenile hormone: nuclear receptors seven-up and FTZ-1 control juvenile hormone biosynthesis during hemimetabolan metamorphosis 11:30 - 11:55 Vincent Henrich, University of North Carolina at Greensboro, USA Transcriptional regulation of two Drosophila early puff genes in the larval salivary gland by 20-E and juvenile hormone: the role of Met and GCE 12:00 – 12:25 Aaron Bauman, HHMI Janelia Farms USA Subfunctionalization of duplicate juvenile hormone receptors in higher Diptera 12:30 - 2:30 Lunch Coffman Union Session X Hormonal Control of Reproduction and Evolution (Chair: Thomas Flatt) 2:30 - 2:25 Thomas Flatt, University of Lausanne, Switzerland The contribution of Endocrine genes to Latitudinal Population Differentiation in Drosophila 2:30 - 2:55 David Dolezel, ENTU Biology Center, Czech Republic Juvenile hormone and circadian genes regulate reproductive diapause in Pyrrhocoris apterus Thursday, July 25 continued 3:00 - 3:25 Mark Brown, University of Georgia, USA Ovary ecdyseroidogenic hormone: function and signaling in mosquitoes 3:30 - 3:55 S. Reddy Palli, University of Kentucky, USA Hormonal Regulation of Reproduction in the Red Flour Beetle, Tribolium castaneum 4:00 -5:00 Business Meeting: Planning the next workshop 2015? 5:00 -6:00 Reception for Alex Raikhel, Indoor Club, TCF Bank Stadium 2009 University Avenue S.E. Minneapolis 6:00 - 6:50 Karlson Lecture: Alex Raikhel The role of JH and 20-E in Mosquito reproduction 7:00 – 9:00 Banquet buffet, Indoor Club TDF Bank Stadium Friday, July 26 8:00 - 8:30 Breakfast: Comstock Dinning Hall or outside meeting room Session XI Ecdysone biosynthesis and regulation (Chair: Marek Jindra) 8:30 - 8:55 Marek Jindra, Biology Centre ASCR, Czech Republic Ecdysoneless -Why is it ecdysone-less? 9:00 - 9:25 Sora Enya, University of Tsukuba, Japan A novel Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis in the prothoracic gland 9:30 - 9:55 Hajime Ono, Kyoto University, Japan Characterization of 3-oxo steroids as intermediates in the Black Box of the ecdysone biosynthetic pathway 10:00 – 10:30 coffee break Session XII Young Investigators (Chair, Arash Bashirullan) 10:30 - 10:55 Arash Bashirullah, University of Wisconsin, USA Forward Genetic Analysis of Ecdysone-Triggered responses During Metamorphosis 11:00 - 11:25 Viviane Callier, Arizona State University Oxygen affects the developmental physiology of growth and metamorphosis initiation in Drosophila 11:30 - 11:55 Megha, National Centre for Biological Sciences, India A role for the IP3 receptor in neuropeptide-producing cells of Drosophila during larval development 12:00 - 12:25 Michael Texada, HHMI Janelia Farms USA Mapping the Drosophila peptide-hormone system Closing remarks End of conference