Tumor-Infiltrating Immune Cell Marker Guide for IHC (Human)Tumor-Infiltrating Immune Cell Marker Guide (Human) Leukocytes CD45+ Myeloid Cells Lymphoid Cells + Class CD11b Myeloid-Derived Plasmacytoid Conventional Granulocytes Macrophages Monocytes T Cells NKT Cells NK Cells B Cells Plasma Cells Supressor Cells (MDSCs) Dendritic Cells Dendritic Cells Siglec-H+and CD11c+and CD68+and CD14+and CD3+ CD56+and CD56+and CD19+ BCMA+or + + +and +and + – + Cell Type CD317 HLA-DR HLA-DR HLA-DR CD3 CD3 Naïve CD138 – – Activated CD11c CD206 and IgD+and CD86– CD27– Neutrophils Monocytic CD83+ CD1c+ M1-like Helper Type I PB Cytotoxic + + CD16 and (M) MDSCs DCs CD86 or T Cells (Th) NKT (iNKT) NK Cells Switched + – + + + + CD66b or CD15 and CD1c CD80 or CD4 TCR Vα24 Vβ11 CD56 low and Memory + + + + CD15/SSEA1 CD14 and iNOS CD16 IgD–and – – Th1 HLA-DR CD1c and Type II Cytotoxic CD27+ + Eosinophils CD141–or M2-like T-Bet NKT Granzyme+ CD193+and Polymorphonuclear CLEC9A– CD163+or Unswitched Th2 + + + or Perforin Siglec-8 and (PMN) MDSCs DCs CD206 GATA-3+ IgD+and – + – CD16 CD15 and CD1c and CD27+ CD14–and CD141– or Th17 PB Immature 18-IMM-18284-FLY1-E1 + – – or RORγt / Regulatory Basophils HLA-DR CLEC9A + – NK Cells FcεR1α and XCR1 Treg – high CD117 + CD56 and CD141+or FoxP3 and CD16–and Activated + + CLEC9A CD25 + Subtypes NCR CD63+and DCs CD203c+ (excel at cross- Cytotoxic Activated presentation) T Cells + CD69+ CD141 or CD8+ Mast cells CLEC9A+or + FcεR1α and XCR1+ Activated Cytotoxic Naïve E ector Memory CD117+and Activated CD69+or Granzyme+ CD45RA+and CD45RO+and Tryptase+ Key CD25+ or Perforin+ CD62L+or CCR7+ CD62L–or CCR7– + + positive/high expression Activated CD83 – Central Memory E ector Exhausted negative/low expression CD203c+ CD45RO+and CD45RA+and PD-1+and TIM-3+ APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence /F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin /-F Frozen /E-P Peptide ELISA / H human / M mouse / R Functional rat / Hm hamster State Markers CD62L+or CCR7+ CD62L–or CCR7– and LAG3+ Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish /B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology For Research Use Only. Not For Use In Diagnostic Not For Procedures. Research Use Only. For rev. 11/20/18 For pathway key and background, please visit: cst-science.com/humanmarkers Key Products for Human Tumor-Infiltrating Immune Cell Markers for IHC Visit www.cellsignal.com for full listing of antibodies, related kits, and reagents. ANTIBODIES APPLICATIONS REACTIVITY #93668 Arginase-1 (D4E3M™) XP® Rabbit mAb WB, IHC-P, IF, F H, M, R #85061 CD3ε (D7A6E™) XP® Rabbit mAb WB, IP, IHC-P H #85336 CD8α (D8A8Y) Rabbit mAb WB, IP, IHC-P, F H #45581 CD11c (D3V1E) XP® Rabbit mAb WB, IHC-P H #75181 CD14 (D7A2T) Rabbit mAb IHC-P H (IHC Formulated) #24326 CD16 (D1N9L) Rabbit mAb IHC-P H #90176 CD19 (D4V4B) XP® Rabbit mAb WB, IP, IHC-P H, M, (B) #37259 CD44 (E7K2Y) XP® Rabbit mAb WB IHC-P H, M, R #3570 CD44 (156-3C11) Mouse mAb WB, IP, IHC-P, IF-IC, F H Arginase-1 (D4E3M™) XP® Rabbit mAb ® CD86 (E2G8P) Rabbit mAb #91882: IHC #13917 CD45 (D9M8I) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F H CD3ε (D7A6E™) XP Rabbit mAb #85061: #93668: IHC analysis of paraffin-embedded IHC analysis of paraffin-embedded human breast analysis of paraffin-embedded human lung #55618 CD45RO (UCHL1) Mouse mAb IHC-P H human colon adenocarcinoma using #93668 carcinoma using #85061. adenocarcinoma using #91882 performed on the (IHC Specific) performed on the Leica® BOND™ Rx. Leica® BOND™ Rx. #55051 CD63 (D4I1X) Rabbit mAb (IHC Specific) IHC-P H #76437 CD68 (D4B9C) XP® Rabbit mAb IHC-P, IF-IC, F H #91882 CD86 (E2G8P) Rabbit mAb WB, IHC-P, IF-IC H #93498 CD163 (D6U1J) Rabbit mAb WB, IHC-P H #91992 CD206/MRC1 (E2L9N) Rabbit mAb WB, IHC-P H #98377 FoxP3 (D2W8E™) Rabbit mAb IHC-P H (IHC Specific) #5852 GATA-3 (D13C9) XP® Rabbit mAb WB, IHC-P, IF-IC, F, ChIP H, M, (Mk) #96098 GATA-3 (E2N1Y) Mouse mAb WB, IHC-P, IF-IC, F H #46890 Granzyme B (D6E9W) Rabbit mAb WB, IHC-P H, M 18-IMM-18284-FLY1-E1 #49420 ITGAM/CD11B (D6X1N) Rabbit mAb WB, IHC-P H #37805 c-Kit (D3W6Y) XP® Rabbit mAb WB, IP, IHC-P H #15372 LAG3 (D2G4O™) XP® Rabbit mAb WB, IHC-P H CD163 (D6U1J) Rabbit mAb #93498: IHC PD-1 (D4W2J) XP® Rabbit mAb #86163: TIM-3 (D5D5R™) XP® Rabbit mAb #45208: #3576 NCAM (CD56) (123C3) Mouse mAb WB, IHC-P, IF-IC, F H analysis of paraffin-embedded human non-small IHC analysis of paraffin-embedded human colon IHC analysis of paraffin-embedded human lung #86163 PD-1 (D4W2J) XP® Rabbit mAb WB, IP, IHC-P, F H cell lung carcinoma using #93498 performed on carcinoma using #86163. carcinoma using #45208. Note staining of the Leica® Bond™ Rx. alveolar macrophages. #43248 PD-1 (EH33) Mouse mAb (IHC-Specific) IHC-P H #13232 T-bet/TBX21 (D6N8B) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP, H ChIP-seq #45208 TIM-3 (D5D5R™) XP® Rabbit mAb WB, IP, IHC-P, F H #88183 TNFRSF17/BCMA (E6D7B) Rabbit mAb WB, IP, IHC-P, F H APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence /F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin /-F Frozen /E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish /B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology For Research Use Only. Not For Use In Diagnostic Not For Procedures. Research Use Only. For © 2019 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners. For pathway key and background, please visit: cst-science.com/humanmarkers.