NAME ___________________________________________________ LAB SECTION _______ LAB TIME ___________________ SEAT NUMBER ________ ADDRESS ______________________________________ ______________________________________ ______________________________________ PHONE NUMBER ________________________ VERTEBRATE DEVELOPMENT, BIOL 4410 LABORATORY HANDOUTS FALL 2016 (revised 2/2/16) INSTRUCTOR - DR. STEPHEN C. KEMPF DEPARTMENT OF BIOLOGICAL SCIENCES AUBURN UNIVERSITY 1 TABLE OF CONTENTS LECTURE, LAB READING, SLIDE #s, AND EXAM SCHEDULE ------------------------ 3 COURSE POLICIES -------------------------------------------------------------------------------- 10 LABORATORY REQUIREMENTS/INFORMATION --------------------------------------- 11 LAB NOTEBOOK REQUIREMENTS ----------------------------------------------------------- 12 LAB NOTEBOOK – FREQUENTLY ASKED QUESTIONS ------------------------------- 14 POSSIBLY USEFUL STUDY HINTS ----------------------------------------------------------- 16 LAB EXAM AND QUIZ SAMPLE QUESTIONS --------------------------------------------- 17 HANDOUT 1A, LABORATORY ORIENTATION ------------------------------------------- 20 HANDOUT 1B, USE OF THE COMPOUND MICROSCOPE ------------------------------- 23 HANDOUT 2A, ROUTINE METHODS --------------------------------------------------------- 29 HANDOUT 2B, MITOSIS, MEIOSIS, AND GAMETOGENESIS -------------------------- 35 HANDOUT 2C, BASIC MICROSCOPY METHODS ----------------------------------------- 37 LABORATORY ID LISTS – OVERVIEW ----------------------------------------------------- 42 HANDOUT 3A, REPRODUCTIVE ORGANS: SPERMATOGENESIS ------------------- 43 HANDOUT 3B, REPRODUCTIVE ORGANS: OOGENESIS ------------------------------- 44 HANDOUT 4A, STARFISH DEVELOPMENT ----------------------------------------------- 45 HANDOUT 4B, EARLY FROG DEVELOPMENT -------------------------------------------- 46 HANDOUT 5, 4 - 7 MM FROG TADPOLE ----------------------------------------------------- 48 HANDOUT 6, 10 MM FROG TADPOLE -------------------------------------------------------- 51 HANDOUT 7A, CRANIAL NERVES AND GANGLIA -------------------------------------- 54 HANDOUT 7B, 18 AND 24 HOUR CHICK ----------------------------------------------------- 58 HANDOUT 8, 33 HOUR CHICK ------------------------------------------------------------------ 60 HANDOUT 9, 48 HOUR CHICK ------------------------------------------------------------------ 62 HANDOUT 10, 72 HOUR CHICK ----------------------------------------------------------------- 65 HANDOUT 11, 96 HOUR CHICK ----------------------------------------------------------------- 72 HANDOUT 12, 6 MM PIG -------------------------------------------------------------------------- 78 HANDOUT 13, 10 MM PIG ------------------------------------------------------------------------- 83 HANDOUT 14, TOOTH DEVELOPMENT ------------------------------------------------------- 90 2 VERTEBRATE DEVELOPMENT - BIOL 4410 LECTURE and LAB FALL 2016 - LECTURE AND LAB TOPICS, STUDY ASSIGNMENTS C - Carlson (6th edition), S - Schoenwolfe (7th edition), D - Digital Lab Manual (If you have a different edition of the text, the required page numbers may be different.) ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Aug 17 W Class orientation, drops and adds, lab switches Introduction, Developmental biology as a science Gametogenesis I: Gametes, where do they come from. C: pp. 1-56, pp. 57-74 W/Th NO LAB TODAY! ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Aug 19 F Finish Introduction, Developmental biology as a science Gametogenesis I: Gametes, where do they come from. C: pp. 1-56, pp. 57-74 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Aug 22 M Gametogenesis I: Gametes, where do they come from C: pp. 57-74 M/T ATTENDANCE AT THIS LAB IS REQUIRED!!!! Lab: Equipment assignments. Use of microscope. D: Introductory materials, Approaches to learning Routine methods of Microtechnique Microscopy: Use of the Microscope ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Aug 24 W Gametogenesis II: Spermatogenesis C: pp. 75-93 W/Th ATTENDANCE AT THIS LAB IS REQUIRED!!!! Lab: Histological sections, a 2-dimensional view of 3-dimensions. Reproductive organs. Tray #1 & 2. D: Developmental Events and Mechanisms, General Background Information, Gametogenesis, Fertilization M: pp. 1-15, 74-77, 126-129 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Aug 26 F Gametogenesis II: Spermatogenesis . C: pp. 75-93 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ 3 Aug 29 M Gametogenesis II: Finish Spermatogenesis. Start Oogenesis C: pp. 75-93 C: pp. 94-120 M/T Lab: Starfish development. Tray #3. Quiz 1 D: Starfish Development, Descriptive Text M: pp. 50-56 FIRST LAB QUIZ TODAY! ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Aug 31 W Gametogenesis III: Oogenesis C: pp. 94-120 W/T h Lab: Early frog development. Tray #4. Quiz 2 D: Amphibian Development, Early Frog Development, Descriptive Text M: pp. 78, 81-96 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 2 F Gametogenesis III: Oogenesis C: pp. 94-120 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 5 M LABOR DAY HOLIDAY 15th day of classes tomorrow ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 7 W Finish Fertilization, C: pp. 121-142 W/Th Lab: 4mm Frog tadpole. Tray #5 Quiz 3 D: Amphibian Development, 4mm Frog Tadpole, Descriptive Text for Wholemount and Transverse sections M: 97-105 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 9 F Fertilization C: pp. 121-142 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 12 M Cleavage. C: pp. 143-150, 151 - 188 M/T Lab: Frog development, 4-7 mm, Tray #5. Quiz 4 D: Amphibian Development, 7mm Frog Tadpole, Descriptive Text for Wholemount and Transverse sections M: pp. 97-105, 106-116 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ 4 Sept 14 W Cleavage. C: pp. 143-150, 151 - 188 . W/Th Lab: Frog development, 4-7 mm, Tray #5. Quiz 5 D: Amphibian Development, 7mm Frog Tadpole, Descriptive Text for Wholemount and Transverse sections M: pp. 97-105, 106-116 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 16 F Cleavage. C: pp. 143-150, 151 - 188 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 19 M Gastrulation. C: pp. 189-226 M/T Lab: Frog development, 10mm. Tray #7. Quiz 6 D: Developmental Events and Mechanisms, Cleavage D: Amphibian Development, 10mm Frog Tadpole, Descriptive Text for Wholemount and Transverse sections M: pp. 117-123 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 21 W FIRST LECTURE EXAM (through Monday's lecture) W/Th Lab: Frog development, 10mm. Tray #7. Quiz 7 D: Developmental Events and Mechanisms, Cleavage D: Amphibian Development, 10mm Frog Tadpole, Descriptive Text for Wholemount and Transverse sections M: pp. 117-123 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 23 F Gastrulation. C: pp. 189-226 ________________________________________________________________________ ------------------------------------------------------------------------------------------------------------ Sept 26 M Gastrulation. C: pp. 189-226 M/T Lab: Chicken development, 18 hr, 24 hr. (4 somite) Tray #8. Quiz 8 D: Developmental Events and Mechanisms, Gastrulation D: Avian Development, Major Events in Early Avian Development Descriptive Text D: Avian Development, 18 hr and 24 hr chick embryo Descriptive Text for 18 hr and 24 hr