Biological and Chemical Safety Manual Fall, 2012 1 EMERGENCY INFORMATION (Fill out laboratory specific information and post outside of laboratory – copy to EHS dept.) Principal Investigator After -Hours Contact Information Lab Location Location Of Chemical Spill Kit Location Of Biological Spill Kit Location Of Fire Extinguisher Location Of Fire Alarm Location of Eye Wash Station Location of Safety Shower USD Environmental Health and Safety Chemical & Radiation Safety Kevin O’Kelley, Director of Environmental Health & Safety [email protected] , 605-677-6265 Institutional Review Board (IRB) Sandra Ellenbolt, Director of Human Subjects Protection [email protected] , 605-677-6067 Institutional Animal Care and Use Committee (IACUC) Peter Autenried, Director of Animal Resource Center [email protected] , 605-677-5174 Institutional Biosafety Committee (IBC) Victor Huber, Ph.D. [email protected] , 605-677-5163 Facilities Management Call Desk: 605-677-5341 (After hours, call USD Public Safety) USD Public Safety Office: 605-677-5342 Fire/Police/Emergency Medical Services Dispatch: 9-911 2 Preface A safe working and learning environment is an expectation of USD and is provided to all employees, students, and guests at the highest level reasonably possible. The University of South Dakota Biological and Chemical Safety Policy is designed as a reference for individual laboratories to provide a safe and productive work environment while complying with applicable federal and state rules and best practices. Each laboratory group should supplement this policy when necessary to insure health and safety of workers is not compromised. The policy is divided into Chemical Safety and Biological Safety sections. Radiation Safety is addressed in the University of South Dakota’s Radiation Safety Policy for Authorized Workers. The information in the Chemical Safety section is in accordance with guidance found in the National Research Council’s Prudent Practices in the Laboratory (National Academy Press, 1995) and the Occupational Safety and Health Administration (OSHA) Occupational Exposures to Hazardous Chemicals in Laboratories (29 CFR 1910.1450). The Chemical Safety section outlines the University of South Dakota’s Chemical Hygiene Plan. The Biological Safety section, in conjunction with the Centers for Disease Control and Prevention (CDC) / National Institutes of Health (NIH) Biosafety in Microbiological and Biomedical Laboratories (BMBL), provides the requirements for safely working with biohazardous materials at The USD. The primary concern when working with hazardous chemicals and pathogens is the safety of personnel working with these agents and the prevention of releases of these agents. While this guide is a sufficient starting point, some laboratories may need to supplement this guide with their own workplace specific standard operating procedures (SOPs). The Principle Investigator (PI) has primary responsibility for ensuring the safety of students, faculty, staff, visitors and the environment with respect to their laboratory operations. The Environmental Health and Safety Office can provide guidance or information for questions regarding chemical and biological Safety. 3 Contents Scope and Applicability 5 General Laboratory Practices and Safety Equipment 7 Personal Protective Equipment (PPE) 10 Fume Hoods and Biosafety Cabinets 12 Laboratory Equipment Safety 14 Emergencies and Accidents 27 Exposure Monitoring and Medical Treatment 29 Training 29 Chemical Safety 31 Understanding Chemical Hazards 31 Material Safety Data Sheets (MSDS) and Labels 38 Laboratory Safety Procedures 42 Chemical Disposal 50 Bio logical S afety 52 Aerosol-Generating Processes 57 Biological Safety Cabinets (Tissue Culture Hoods) 58 BSC Use: Work Practices and Procedures 65 General Biosafety Issues 69 Biological Spills and Decontamination 70 Regulated Medical/Bio-Hazardous Waste Management 71 Summary of Biosafety Levels 73 Summary of Agents 76 Appendices A Example Glove Compatibility Chart 78 B NIOSH Guide on Chemical Storage 79 C Biological Safety Self-Audit Form 83 4 Scope and Applicability The University of South Dakota is committed to preserving the health and safety of its students, staff and faculty and to protecting the environment and community. It is recognized that the use of hazardous chemicals, potentially pathogenic microorganisms, infectious agents, human tissue and bloodborne pathogens, and/or organisms containing recombinant DNA (rDNA) is necessary in many university research and teaching laboratories. To ensure the safe handling of these agents, the University of South Dakota requires compliance with the NIH Guidelines for Research involving rDNA Materials; BMBL; Occupational Safety and Health Administration (OSHA) standards (i.e., Title 29 CFR Part 1910.1030 and 1910.1450) and with the recommendations provided in the Biological and Chemical Safety Policy. Responsibilities The Institutional Biosafety Committee (IBC) is charged by the President of The University of South Dakota to formulate policy and procedures related to the use of biohazardous agents and materials. Hazardous biological agents consist of viruses, bacteria, fungi, and non-exempt recombinant DNA organisms listed by the Center for Disease Control and Prevention (CDC) and/or the US Department of Agriculture (USDA). The IBC responsibility does not extend to toxic materials that are not toxins. The Director, Environmental Health and Safety (EHS) is responsible for implementing the University’s radiation, chemical and biological safety programs. In this role, the director functions as the University’s Radiation Safety Officer (RSO), Chemical Hygiene Officer (CHO), and Biological Safety Officer, if the University engages in rDNA research at the Biosafety Level 3). The Director, EHS will: • monitor compliance with The University safety practices and procedures regarding chemical and biohazardous materials • work with faculty and staff to develop and implement appropriate safety practices and policies • ensure that safety audits are preformed periodically and that results are reported to the responsible parties • provide consultation to investigators on matters relating to laboratory safety, appropriate handling and containment of hazardous materials, decontamination and disposal of hazardous wastes • serve as liaison between the University and outside regulatory agencies concerning the use of hazardous materials Ultimate responsibility for the safe conduct of research involving hazardous materials rests with the laboratory supervisor or principal investigator (PI). Each faculty member is responsible for complying with the requirements in this Policy and implementing all necessary precautions to prevent undesirable consequences of experimental work conducted in the laboratory. The PI will: • monitor daily operations of the laboratory • inform / train persons who enter the laboratory and document the training for each person who works in the lab. • ensure that laboratory workers understand and follow the Biological and Chemical Safety Policy and any other laboratory specific safety procedures 5 • maintain a proper inventory and storage of chemicals and biohazardous materials • determine the required level of protective apparel and equipment for a given procedure and insure proper protective equipment is used • insure that laboratory equipment is adequate for any material being ordered and that training is conducted on the hazards associated with the use of any material • establish written operation and decontamination procedures • establish emergency procedures and train all workers on emergency response • insure good housekeeping and chemical / biological hygiene practices are maintained in the laboratory and that it is kept free from clutter and debris • conduct regular laboratory safety and housekeeping inspections, including routine inspections of emergency equipment • report accidents and any other incident involving spill / release, injury, exposure, etc. following University policies • arrange for immunizations and/or health surveillance of laboratory personnel if deemed appropriate for the research project • all design, construction and/or modification of space must be reviewed and approved by the Director of Facilities Management; the USD will not permit renovations that result in unsafe or unhealthy working environments or do not meet national codes While the Director, EHS and the PI are responsible for insuring a safe work site, the laboratory worker is responsible for keeping themselves informed of the risks involved from working in a laboratory. The laboratory worker will: • follow all safety and health procedures in the Biological and Chemical Safety Policy and any procedures developed by the faculty supervisor in the laboratory • complete required health and safety training sessions. • wear all required personal protective equipment and develop good personal laboratory safety habits • report accidents, injuries and unsafe conditions to the PI or the laboratory supervisor A university is a learning institution, visitors are to be expected. The laboratory supervisor or PI is responsible to insuring the safety of all visitors in the laboratory. High school students destined to work in a laboratory must be trained and their parents must be appraised of the risk and approve, in writing, of the relationship. Children under the age of 18 are prohibited from hazardous materials laboratories unless prior approval has been obtained from the department head and EHS.