Bravo Automated Liquid-Handling Platform User Guide Agilent Technologies ,Notices © Agilent Technologies, Inc. 2009 Warranty (June1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any No part of this manual may be reproduced The material contained in this docu- technical data. in any form or by any means (including ment is provided “as is,” and is sub- electronic storage and retrieval or ject to being changed, without notice, Safety Noticies translation into a foreign language) without prior agreement and written consent from in future editions. Further, to the max- A WARNING notice denotes a Agilent Technologies, Inc. as governed by imum extent permitted by applicable hazard. 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U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 Contents Preface . vii About this guide . viii Accessing Automation Solutions user information. ix 1. Safety guidelines . 1 General safety information . 2 Stopping in an emergency . 3 Potential safety hazards. 4 2. Introduction to the Bravo Platform . 7 About the Bravo Platform . 8 Hardware overview. 10 Indicator lights. 15 Connection panel description . 16 Pipette heads and pin tools . 18 Software overview . 21 3. Installing the Bravo Platform. 23 Workflow for installing the Bravo Platform . 24 Laboratory setup requirements. 25 Unpacking and inspecting the Bravo Platform. 27 Connecting the Bravo Platform. 32 Installing the pipette head or pin tool . 36 4. Setting up the Bravo Platform . 41 Workflow for setting up the Bravo Platform. 42 Starting up and shutting down . 43 Creating or adding a Bravo device . 45 Opening Bravo Diagnostics . 49 Creating and managing profiles . 51 Bravo concurrent motion features for integrated systems. 56 Initializing the Bravo Platform. 58 Setting teachpoints. 61 Verifying the gripper setup . 68 Preparing the Bravo Platform for a run . 70 5. Maintaining the Bravo Platform . 73 Routine maintenance . 74 Bravo Automated Liquid-Handling Platform User Guide iii Contents Cleaning the Bravo Platform. 75 Cleaning up after a run . 76 Changing the pipette head or pin tool. 77 Replacing the fuse . 82 Retracting tip-box stripper pins . 84 Moving the pipette head manually . 86 Recovering from a head collision. 87 Troubleshooting hardware problems . 89 Troubleshooting hardware-related error messages . 91 Reporting problems . 92 6. Using Bravo.