ABSTRACTS 2 ANIMAL BIOLOGY ANNUAL MEETING SEVILLE 2019 ANIMAL BIOLOGY ABSTRACTS 82 ANNUAL MEETING SEVILLE 2019 ANIMAL BIOLOGY ABSTRACTS 83 t ra n spor t to t he c y tosol f rom t he e x t racel lu la r med iu m (Slc7a11) and A1 TRANSGENERATIONAL RESPONSE A1.3 IS THERE A MECHANISTIC LINK from melanosomes (CTNS), and regulation of tyrosinase activity BETWEEN DNA METHYLATION AND (MC1R and ASIP). These changes were mediated by increases in TO ENVIRONMENTAL STRESS: REVERSIBLE ACCLIMATION? DNA m 5 C in all genes except Slc7a11, wh ic h e x pe r ienced R NA m 6 A depletion. Birds supplemented with cysteine synthesized more pheomelanin than controls, but did not suffer higher systemic DEVELOPMENTAL PROGRAMMING TUESDAY 2 JULY, 2019 09:45 ox idat ive st ress. T hese resu lt s suggest t hat e xcess c ystei ne ac t ivates an epigenetic mechanism that favours pheomelanin synthesis and AND LIFETIME FITNESS FRANK SEEBACHER (UNIVERSITY OF SYDNEY, AUSTRALIA) may protec t aga i n st ox idat ive st ress. [email protected] Phenotypic plasticity can confer resilience to environmental change. Understanding the mechanistic basis of plasticity is A1.5 EVOLUTIONARY INSIGHTS i mpor ta nt to pred ic t it s ma n i festat ion i n a phylogenet ic conte x t, NTO TRANSGENERATIONAL EFFECTS and under different scenarios of climate change. Plasticity of OF PESTICIDES AMÉLIE CRESPEL (UNIVERSITY OF GLASGOW) physiological functions is obligatory for cell survival, and it ORGANISED BY: emerges from regulatory systems that evolved to maintain TUESDAY 2 JULY, 2019 10:30 AND SHAUN KILLEN (UNIVERSITY OF GLASGOW) energy homeostasis within cells. Interactions between regulators pe r m it ted a n i nc rea si ng ly soph i st icated fi ne -t u n i ng of phenot y pes, VERÓNICA CASTAÑO-SANZ (ESTACIÓN BIOLÓGICA DE DOÑANA and potentially link transgenerational, developmental, and CSIC, SPAIN), IVAN GOMEZ-MESTRE (ESTACIÓN BIOLÓGICA reversible plasticity. We used a zebrafish DNA methyltransferase 3 DE DOÑANA CSIC, SPAIN), FRANCISCO GARCIA-GONZALEZ A1.1 EPIGENETIC INHERITANCE AS A A1.2 PARENTAL DIETARY CRUDE knock-out model to test whether there is a mechanistic link between (ESTACIÓN BIOLÓGICA DE DOÑANA CSIC, SPAIN) BRIDGE FOR SURVIVING SHORT-TERM OIL EXPOSURE IN THE KING QUAIL env i ron menta l ly i nduced DNA met hylat ion i n pa rent s a nd ea rly e mbr yon ic stages, a nd reve rsible acc l i mat ion i n adu lt offspr i ng. ENVIRONMENTAL STRESSORS (COTURNIX CHINENSIS) LEADS TO [email protected] It is constructive to interpret thermal plasticity in the context of EFFECTS ON OFFSPRING RESPIRATORY explicit regulatory mechanisms, which can replace current verbal Pesticides and fertilizers are used all over the world affecting TUESDAY 2 JULY, 2019 09:00 PHYSIOLOGY evolutionary models. a myriad of organisms. Pesticides drive evolution by imposing a selective pressure on organisms, to which they can respond WARREN BURGGREN (UNIVERSITY OF NORTH TEXAS, TUESDAY 2 JULY, 2019 09:30 plastically and eventually adapt. In this work we investigate UNITED STATES) whether and how transgenerational effects triggered by pesticide e x posu re modu late evolut ion of t ra it s i nvolved i n rapid adaptat ion . [email protected] NAIM BAUTISTA (UNIVERSITY OF NORTH TEXAS, UNITED A1.4 CHANGES IN MELANOCYTE STATES), LARA DO AMARAL-SILVA (SAO PAULO STATE RNA AND DNA METHYLATION FAVOUR We will present results of ongoing work in which seed beetles of the pest species Callosobruchus maculatus are exposed to sublethal Epigenet ic i n he r ita nce of mod i fied phenot y pic t ra it s ha s of ten been UNIVERSITY (FCAV-UNESP), BRAZIL), NEAL DAVAR PHEOMELANIN SYNTHESIS AND concent rat ion s of a com mon ly u sed pest ic ide, a nd t he e ffec t s of suc h framed in the context of disease, maladaption or unfit phenotypes, (UNIVERSITY OF NORTH TEXAS, UNITED STATES), WARREN W MAY AVOID SYSTEMIC OXIDATIVE exposure on subsequent generations are examined. This test will especially from a medical perspective. Yet, epigenetically BURGGREN (UNIVERSITY OF NORTH TEXAS, UNITED STATES) inform on whether parental exposure to sublethal concentrations of i n he r ited phe not y pes ge ne rated by pa renta l e x posu re to adve rse STRESS AFTER DIETARY CYSTEINE pest ic ide a ffec t s subseque nt ge ne rat ion s, eve n i f t hose ge ne rat ion s environments can also be highly adaptive when that adverse [email protected] SUPPLEMENTATION IN BIRDS a re not e x posed to t he tox ica nt t hem selves. We w i l l dete r m i ne t he environment persists across several generations. Two key Crude oil represents a constant threat for marine and marshal lifetime reproductive success (LRS), longevity and fecundity of the features of epigenetically inherited traits can make them of critical birds species that could be exposed dermally, dietarily or via TUESDAY 2 JULY, 2019 10:15 pa renta l gene rat ion , a s wel l a s of t he F1 a nd F2 . We e x pec t pest ic ide importance when organisms face prolonged, multi-generational respi rat ion , lead i ng to det r i me nta l e ffec t s on t hei r mor pholog y a nd exposure to alter the fitness traits of the treated (exposed) groups, periods of adverse environments. First, epigenetically inherited physiology. However, oil spills often coincide with the breeding SOL RODRÍGUEZ-MARTÍNEZ (DEPARTAMENTO DE ECOLOGÍA compared to control groups (non-exposed to the pesticide), and traits can arise quickly and simultaneously in most if not all of a sea son for t hese spec ies a nd potent ia l ly a ffec t s t hei r reproduc t ive EVOLUTIVA, ESTACIÓN BIOLÓGICA DE DOÑANA - CSIC, SPAIN), we a lso e x pec t t hat t he pest ic ide’s i mpac t wou ld pe rsi st for some popu lat ion a s a resu lt of com mon i nd iv idua l or pa renta l e x pe r ience. success and developmental trajectories of their offspring. We RAFAEL MÁRQUEZ (DEPARTAMENTO DE ECOLOGÍA EVOLUTIVA, generations but would become attenuated with time. The results This sharply contrasts with genetic inheritance, in which adaptive u sed t he k i ng qua i l a s a n i ma l model a nd e x posed t he m to d i ffe re nt ESTACIÓN BIOLÓGICA DE DOÑANA - CSIC, SPAIN), ÂNGELA will improve our understanding of the consequences of pesticides t ra it s a r i si ng by mutat ion may on ly ve r y slowly become fi xed (or not) concentrations of crude oil (1%HEWAF or 10%HEWAF) via their INÁCIO (LABORATÓRIO DE GENÉTICA, INSTITUTO DE SAÚDE (a nd of ot he r env i ron menta l st ressors a nd tox ica nt s gene ra l ly), a nd across many successive generations. Second, epigenetic traits are by diet. We analyzed indicators of health (body mass, temperature, AMBIENTAL, FACULDADE DE MEDICINA, UNIVERSIDAD DE will show whether these chemicals play a role driving t hei r ve r y nat u re reve rsible. Epige net ic reade rs, w r ite rs a nd e ra se rs oxygen consumption, ventilatory rate,) and fitness (laying, LISBOA, PORTUGAL), ISMAEL GALVÁN (DEPARTAMENTO DE t ra n sge ne rat iona l e ffec t s t hat may u nde rl ie popu lat ion dy na m ics sense and then modify the state of epigenetic markers regulating fertilization, hatching success) in the adult quails, then we assessed ECOLOGÍA EVOLUTIVA, ESTACIÓN BIOLÓGICA DE DOÑANA - and the evolution of resistance. These are key aspects for gene expression, and are highly dynamic as they constantly from the cellular (mitochondrial respiration) to the systemic level CSIC, SPAIN) conservation and agricultural management, and for enhancing reacting to environmental conditions. Changes in epigenetic (respi romet r y) i f t he respi rator y physiolog y of t hei r offspr i ng wa s our understanding of adaptive evolution. w r ite rs a nd e ra se rs mod i f y t he deg ree of DNA met hylat ion state impaired. Preliminary results indicate that body mass does not vary [email protected] or the makeup of non-coding microRNAs, both of which regulate among adult groups (~69.5g female, ~46.4g male) however, oxygen ge ne e x pression . T he resu lt i ng phe not y pe c ha nges ca n i nc lude a Cysteine plays essential biological roles, but excessive amounts con su mpt ion wa s i nc rea sed i n t he g roup e x posed to t he h ig he r dose return to the genetically determined default or normal condition. produce cellular oxidative stress. Cysteine metabolism is compared with control animals (5.95 mL O /g/h and 5.10 mL O /g/h, Thus, epigenetic inheritance of modifed adaptive phenotypes 2 2 mainly mediated by the enzymes cysteine dioxygenase and p < 0.05, respec t ively). A lt houg h egg mass a nd si ze d id not d i ffer ca n be a n effect ive mecha n ism a l low i ng orga n isms i n dy na m ic γ-glutamylcysteine synthetase, respectively coded by the genes among groups, fertilization percentage was reduced in oil-exposed environments to respond to short-term environmental changes.