University of Florida Thesis Or Dissertation Formatting Template

University of Florida Thesis Or Dissertation Formatting Template

THE ECOLOGY AND EVOLUTION OF ARMAMENT DEFENSE VARIATION IN AFRICAN ACACIAS By MEGAN CATHLEEN GITTINGER A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2013 1 © 2013 Megan Cathleen Gittinger 2 To my mom, who always supports my endeavors with healthy skepticism 3 ACKNOWLEDGMENTS I thank James Ekiru, James Olingnengero, John Lemboi, and Moso for research assistance in the field, without Ekiru I may have been trampled by elephants; Mike Littlewood for logistical support at Mpala Research Centre, cheery disposition and endless stories from the earlier days of Mpala and Kenya; Kathleen Rudolph for commiseration in the field research and for moral support along the way; Todd Palmer for feedback and support throughout my dissertation and for the Penicillins; Craig Osenberg for including me in lab meetings; the Research Reviews group for valuable feedback on experimental design; Dr. David Augustine for insightful feedback on herbivore foraging and for allowing me access to long-term exclosure plots at Mpala; Bernard Hauser for insights in plant development; Heather McAuslane for comments and discussions on inducible plant defense; Pamela Soltis for lab-support and guidance on molecular phylogenetics; Julian Resasco and Schuyler van Montfrans for being there from the beginning; Matthew Smith and Adrian Stier for adopting me as friend; Ashley Seifert for being my husband and for always finding what I have to say interesting; and my entire family for providing endless support and for being amazing role models for personal and professional life. 4 TABLE OF CONTENTS page ACKNOWLEDGMENTS .................................................................................................. 4 LIST OF TABLES ............................................................................................................ 8 LIST OF FIGURES .......................................................................................................... 9 ABSTRACT ................................................................................................................... 10 CHAPTER 1 GENERAL INTRODUCTION .................................................................................. 12 Why Study Structural Defense Traits? .................................................................... 12 Why Study Acacia Spines? ..................................................................................... 12 General Overview ................................................................................................... 14 2 HERBIVORES AND PLANT SIZE AS DETERMINANTS OF SPINE DEFENSE MORPHOLOGY IN AN AFRICAN ACACIA ............................................................ 15 Background ............................................................................................................. 15 Methods .................................................................................................................. 17 Study Site ......................................................................................................... 17 Natural Variation in Spine Defenses ................................................................. 18 Herbivory and Spine Defenses ......................................................................... 18 Analysis ............................................................................................................ 19 Results .................................................................................................................... 19 Natural Variation in Spine Defenses ................................................................. 19 Herbivory and Spine Defenses ......................................................................... 20 Herbivory, Spine Defenses, and Plant Size ...................................................... 21 Discussion .............................................................................................................. 22 Relaxation of Spine Defenses following Herbivore Exclusion ........................... 22 Size-based Relationships in Spine Defenses ................................................... 23 3 SPINE VARIATION DETERMINES DEFENSIVE EFFICACY AGAINST BROWSERS OF DIFFERENT SIZES AND FEEDING STRATEGIES ................... 37 Background ............................................................................................................. 37 Methods .................................................................................................................. 40 Study Site and Feeding Trials .......................................................................... 40 Plant Selection and Branch Treatments ........................................................... 41 Biomass Estimates ........................................................................................... 41 Patterns of Biomass Removal .......................................................................... 43 Branch Type Characteristics ............................................................................ 44 Results .................................................................................................................... 44 5 Defense Efficacy of Spines Against Goats and Camels ................................... 44 Patterns of Biomass Removal .......................................................................... 45 Branch Type Characteristics ............................................................................ 46 Discussion .............................................................................................................. 48 Ecological Function and Consequences of Spine Form and Investment .......... 48 Mechanistic Links to Spine Variation ................................................................ 51 Evolutionary Significance of Armament Variation ............................................. 53 4 EVOLUTION OF SPINE FORM AND DOMATIA IN AFRICAN VACHELLIA .......... 59 Background ............................................................................................................. 59 Methods .................................................................................................................. 61 Species Sampling ............................................................................................. 61 DNA Extraction, Amplification and Sequencing ................................................ 62 Phylogenetic Estimation ................................................................................... 62 Trait Evolution .................................................................................................. 63 Results .................................................................................................................... 63 Phylogenetic Estimation ................................................................................... 63 Evolution of Spine Morphology ......................................................................... 64 Discussion .............................................................................................................. 65 Phylogenetic Relationships of African Vachellia ............................................... 65 Independent Origins of Spine Form and Domatia in Vachellia ......................... 66 Evolutionary Convergence of Structural Defense Traits ................................... 68 5 SPINES, PRICKLES AND THORNS, OH, MY! PHYSICAL ARMAMENTS AS STAND UP TRAITS FOR STUDYING PLANT DEFENSES ................................... 80 Background ............................................................................................................. 80 Armament Morphology and Development............................................................... 83 Prickles ............................................................................................................. 84 Spines .............................................................................................................. 85 Thorns .............................................................................................................. 86 Armament as an Effective Form of Resistance ....................................................... 88 Defensive Benefits of Armament ...................................................................... 88 Effects of Armament on Herbivore Feeding Behavior ...................................... 90 Costs of Defensive Armament ................................................................................ 94 Allocation Costs ................................................................................................ 94 Opportunity and Ecological Costs .................................................................... 97 Armament Plasticity ................................................................................................ 99 Responses as Induced Defenses ................................................................... 100 Ontogenetic Shifts in Armament ..................................................................... 102 Physiological Changes and Resource Allocation ........................................... 104 Future Directions .................................................................................................. 106 6 GENERAL CONCLUSIONS ................................................................................. 112 Determinants of Spine Defense

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